ID   ERS1_SCHPO              Reviewed;         956 AA.
AC   O94717;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=RNA-silencing factor ers1;
GN   Name=ers1; ORFNames=SPCC1393.05;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [3]
RP   FUNCTION.
RX   PubMed=18658154; DOI=10.1074/jbc.c800140200;
RA   Rougemaille M., Shankar S., Braun S., Rowley M., Madhani H.D.;
RT   "Ers1, a rapidly diverging protein essential for RNA interference-dependent
RT   heterochromatic silencing in Schizosaccharomyces pombe.";
RL   J. Biol. Chem. 283:25770-25773(2008).
RN   [4]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=20537132; DOI=10.1186/gb-2010-11-6-r60;
RA   Han T.X., Xu X.Y., Zhang M.J., Peng X., Du L.L.;
RT   "Global fitness profiling of fission yeast deletion strains by barcode
RT   sequencing.";
RL   Genome Biol. 11:R60.1-R60.13(2010).
CC   -!- FUNCTION: Involved in RNAi-dependent heterochromatin formation and
CC       centromeric silencing. Required for the conversion of centromeric pre-
CC       small interfering RNA transcripts into small interfering RNAs, histone
CC       H3 'Lys9' methylation, and the recruitment of the RITS complex to
CC       centromeric sequences. {ECO:0000269|PubMed:18658154}.
CC   -!- INTERACTION:
CC       O94717; O74465: hrr1; NbExp=4; IntAct=EBI-15977565, EBI-15624169;
CC       O94717; P40381: swi6; NbExp=3; IntAct=EBI-15977565, EBI-926939;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
CC       Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole
CC       body {ECO:0000269|PubMed:16823372}.
CC   -!- DISRUPTION PHENOTYPE: Leads to sensitivity to thiabendazole and
CC       microtubule depolymerizing drugs. {ECO:0000269|PubMed:20537132}.
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DR   EMBL; CU329672; CAB38161.1; -; Genomic_DNA.
DR   PIR; T40953; T40953.
DR   RefSeq; NP_587963.1; NM_001022954.2.
DR   AlphaFoldDB; O94717; -.
DR   BioGRID; 275275; 118.
DR   DIP; DIP-60034N; -.
DR   IntAct; O94717; 5.
DR   STRING; 4896.SPCC1393.05.1; -.
DR   MaxQB; O94717; -.
DR   PaxDb; O94717; -.
DR   EnsemblFungi; SPCC1393.05.1; SPCC1393.05.1:pep; SPCC1393.05.
DR   GeneID; 2538690; -.
DR   KEGG; spo:SPCC1393.05; -.
DR   PomBase; SPCC1393.05; ers1.
DR   VEuPathDB; FungiDB:SPCC1393.05; -.
DR   HOGENOM; CLU_354942_0_0_1; -.
DR   OMA; AMIENSQ; -.
DR   PRO; PR:O94717; -.
DR   Proteomes; UP000002485; Chromosome III.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0005816; C:spindle pole body; IEA:UniProtKB-SubCell.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
DR   GO; GO:0031048; P:heterochromatin assembly by small RNA; IMP:PomBase.
DR   GO; GO:0031508; P:pericentric heterochromatin assembly; IMP:PomBase.
PE   1: Evidence at protein level;
KW   Cytoplasm; Cytoskeleton; Reference proteome; RNA-mediated gene silencing.
FT   CHAIN           1..956
FT                   /note="RNA-silencing factor ers1"
FT                   /id="PRO_0000303939"
SQ   SEQUENCE   956 AA;  109007 MW;  C489636820E19512 CRC64;
     MGKVSANNQS GFFKFYIDTA LLLNAEFISS FTNCINSEYN GKVKISYSSS SQQFKVIVYE
     EHKREAFSLF EEIVQKLKRE STVYKKPRLD IHPFGQLHYD FTTLLNFQRY LDLGYGIEDV
     KIKENEDIVK SKVGKKPTSS DFYIPCLIKP KIITPFEWEG NFKQVIYSPT VYNGSFKRLF
     YLPDNNDLLK EFAHATNTKY KASLKDNKIV VLANNDQDIN AMLEKMKHIE NLVSQKVYEY
     PEETLVYAGK IAPCQLKFLK LKFENEILYT AMHTTSIFKS VFNILGSFYT MRLLRNKHDG
     DSYELVPVFA KNPSKTPPCN DNFKIFSDDI KLIGCKIFAG VSLNFSSPKP AHRFYELNKT
     SSNLSIPVLQ KPSNFHSSST ELSDNSIHQG RRAVDPVVNQ NNPSNFEEMI MNKLNKLPTI
     DKQILGTSSL THFQDKTTAI EHSINKSNSK QPPRFKFQLP PRPTSNTLPL EPEEELVTRY
     SVSSDGNTVD EAITKQSQTF QLVNSNEFNE VNANDVHKSL RQNCAKLDFD DSKSKNLLSV
     ECLELDKGSD CSTPKSGSLT PSIDMKFLRL QDEKMDDLGD NYYTILMSSN PVSSYGVGSL
     YLFQPKIVCS EKYINHEEID NMNLKSLHRW LSRSLHVLQS FSGEIELNLE FGVILYPNIS
     SDVSACSHGF MNIYKDLNLP RSYFADCITK SVSNIDSLLN TPVKILFGRT EYTYPLIEHE
     VLDSKNYFVF KGSLLFTDDK RNKTEDSTVF FSMICSSKLD QFAFYKDSKQ SSTCTINFPL
     ALWDSRLRIE TKVPLNDAIL TEFSKSIRFR NVNKDLLLVF GNMEDRVIIH SVTRINENSV
     PFNKNLLPHS LEFILKCSKV KSYDISPSSI NSKEAFVCLD RSKESKPIES CFSLSIQSVY
     MQSQFKYNNS IRAGETAPWK LANKQFIGGA ITENVSDLYT AAIIMVNQLG GIHPTI