ERS1_YEAST
ID ERS1_YEAST Reviewed; 260 AA.
AC P17261; D6VR77;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Cystine transporter;
DE AltName: Full=ERD suppressor;
DE AltName: Full=Transmembrane protein ERS1;
GN Name=ERS1; OrderedLocusNames=YCR075C; ORFNames=YCR75C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2186379; DOI=10.1093/nar/18.8.2177;
RA Hardwick K., Pelham H.;
RT "ERS1 a seven transmembrane domain protein from Saccharomyces cerevisiae.";
RL Nucleic Acids Res. 18:2177-2177(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1574125; DOI=10.1038/357038a0;
RA Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA Sgouros J.G.;
RT "The complete DNA sequence of yeast chromosome III.";
RL Nature 357:38-46(1992).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION, AND FUNCTION.
RX PubMed=15885099; DOI=10.1111/j.1742-4658.2005.04670.x;
RA Gao X.-D., Wang J., Keppler-Ross S., Dean N.;
RT "ERS1 encodes a functional homologue of the human lysosomal cystine
RT transporter.";
RL FEBS J. 272:2497-2511(2005).
CC -!- FUNCTION: Cystine/H(+) symporter that mediates export of cystine, the
CC oxidized dimer of cysteine, from vacuoles/endodomes.
CC {ECO:0000269|PubMed:15885099}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + L-cystine(out) = H(+)(in) + L-cystine(in);
CC Xref=Rhea:RHEA:66172, ChEBI:CHEBI:15378, ChEBI:CHEBI:35491;
CC Evidence={ECO:0000250|UniProtKB:O60931};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:66173;
CC Evidence={ECO:0000250|UniProtKB:O60931};
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000269|PubMed:15885099};
CC Multi-pass membrane protein {ECO:0000269|PubMed:15885099}. Vacuole
CC membrane {ECO:0000269|PubMed:15885099}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15885099}.
CC -!- SIMILARITY: Belongs to the cystinosin family. {ECO:0000305}.
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DR EMBL; X52468; CAA36706.1; -; Genomic_DNA.
DR EMBL; X59720; CAA42264.1; -; Genomic_DNA.
DR EMBL; BK006937; DAA07546.1; -; Genomic_DNA.
DR PIR; S22850; S22850.
DR RefSeq; NP_010000.1; NM_001178784.1.
DR AlphaFoldDB; P17261; -.
DR BioGRID; 31050; 143.
DR DIP; DIP-8250N; -.
DR IntAct; P17261; 4.
DR MINT; P17261; -.
DR STRING; 4932.YCR075C; -.
DR TCDB; 2.A.43.1.3; the lysosomal cystine transporter (lct) family.
DR PaxDb; P17261; -.
DR PRIDE; P17261; -.
DR DNASU; 850438; -.
DR EnsemblFungi; YCR075C_mRNA; YCR075C; YCR075C.
DR GeneID; 850438; -.
DR KEGG; sce:YCR075C; -.
DR SGD; S000000671; ERS1.
DR VEuPathDB; FungiDB:YCR075C; -.
DR eggNOG; KOG3145; Eukaryota.
DR GeneTree; ENSGT00390000005338; -.
DR HOGENOM; CLU_046327_0_0_1; -.
DR InParanoid; P17261; -.
DR OMA; FSFYPQI; -.
DR BioCyc; YEAST:G3O-29374-MON; -.
DR Reactome; R-SCE-425393; Transport of inorganic cations/anions and amino acids/oligopeptides.
DR Reactome; R-SCE-5223345; Miscellaneous transport and binding events.
DR PRO; PR:P17261; -.
DR Proteomes; UP000002311; Chromosome III.
DR RNAct; P17261; protein.
DR GO; GO:0005768; C:endosome; IDA:SGD.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000324; C:fungal-type vacuole; IDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR GO; GO:0015184; F:L-cystine transmembrane transporter activity; IGI:SGD.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015811; P:L-cystine transport; IDA:SGD.
DR InterPro; IPR005282; LC_transporter.
DR InterPro; IPR006603; PQ-loop_rpt.
DR PANTHER; PTHR13131; PTHR13131; 1.
DR Pfam; PF04193; PQ-loop; 2.
DR SMART; SM00679; CTNS; 2.
DR TIGRFAMs; TIGR00951; 2A43; 1.
PE 3: Inferred from homology;
KW Endosome; Glycoprotein; Membrane; Reference proteome; Repeat; Symport;
KW Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..260
FT /note="Cystine transporter"
FT /id="PRO_0000205519"
FT TRANSMEM 7..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 40..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 1..67
FT /note="PQ-loop 1"
FT DOMAIN 162..212
FT /note="PQ-loop 2"
FT CARBOHYD 177
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 260 AA; 30116 MW; BF9E129E44D20655 CRC64;
MVSLDDILGI VYVTSWSISM YPPIITNWRH KSASAISMDF VMLNTAGYSY LVISIFLQLY
CWKMTGDESD LGRPKLTQFD FWYCLHGCLM NVVLLTQVVA GARIWRFPGK GHRKMNPWYL
RILLASLAIF SLLTVQFMYS NYWYDWHNSR TLAYCNNLFL LKISMSLIKY IPQVTHNSTR
KSMDCFPIQG VFLDVTGGIA SLLQLIWQLS NDQGFSLDTF VTNFGKVGLS MVTLIFNFIF
IMQWFVYRSR GHDLASEYPL
