ID   ES1R_PELRI              Reviewed;          46 AA.
AC   P86018;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2009, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Esculentin-1R {ECO:0000303|PubMed:18855342};
OS   Pelophylax ridibundus (Marsh frog) (Rana ridibunda).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Pelophylax.
OX   NCBI_TaxID=8406;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, DISULFIDE BOND, AND SUBCELLULAR
RP   LOCATION.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:18855342};
RX   PubMed=18855342; DOI=10.1002/rcm.3759;
RA   Samgina T.Y., Artemenko K.A., Gorshkov V.A., Ogourtsov S.V., Zubarev R.A.,
RA   Lebedev A.T.;
RT   "De novo sequencing of peptides secreted by the skin glands of the
RT   caucasian green frog Rana ridibunda.";
RL   Rapid Commun. Mass Spectrom. 22:3517-3525(2008).
CC   -!- FUNCTION: Shows antibacterial activity against representative Gram-
CC       negative and Gram-positive bacterial species, and hemolytic activity.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:18855342}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000305|PubMed:18855342}.
CC   -!- MASS SPECTROMETRY: Mass=4771; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:18855342};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Esculentin subfamily. {ECO:0000255}.
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DR   AlphaFoldDB; P86018; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR012521; Antimicrobial_frog_2.
DR   Pfam; PF08023; Antimicrobial_2; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial; Cytolysis;
KW   Direct protein sequencing; Disulfide bond; Hemolysis; Secreted.
FT   PEPTIDE         1..46
FT                   /note="Esculentin-1R"
FT                   /evidence="ECO:0000269|PubMed:18855342"
FT                   /id="PRO_0000361073"
FT   DISULFID        40..46
FT                   /evidence="ECO:0000269|PubMed:18855342"
FT   UNSURE          11
FT                   /note="L or I"
FT                   /evidence="ECO:0000269|PubMed:18855342"
SQ   SEQUENCE   46 AA;  4776 MW;  CDCAD67F71DFE8EF CRC64;
     GIFSKLAGKK LKNLLISGLK SVGKEVGMDV VRTGIDIAGC KIKGEC