ID   ES2SE_LITSE             Reviewed;          37 AA.
AC   P85055;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 27.
DE   RecName: Full=Esculentin-2SE {ECO:0000303|PubMed:16386333};
OS   Lithobates sevosus (Dusky gopher frog) (Rana sevosa).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=299683;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP   MASS SPECTROMETRY.
RC   TISSUE=Skin secretion {ECO:0000269|PubMed:16386333};
RX   PubMed=16386333; DOI=10.1016/j.peptides.2005.11.021;
RA   Graham C., Richter S.C., McClean S., O'Kane E., Flatt P.R., Shaw C.;
RT   "Histamine-releasing and antimicrobial peptides from the skin secretions of
RT   the dusky gopher frog, Rana sevosa.";
RL   Peptides 27:1313-1319(2006).
CC   -!- FUNCTION: Mast cell degranulating peptide. Causes histamine release
CC       from rat peritoneal mast cells in vitro. Has antibacterial activity
CC       against the Gram-negative bacterium E.coli K12 and Gram-positive
CC       bacterium M.luteus NCT C2665. {ECO:0000269|PubMed:16386333}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16386333}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:16386333}.
CC   -!- MASS SPECTROMETRY: Mass=3837; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16386333};
CC   -!- MASS SPECTROMETRY: Mass=3837; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:16386333};
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Esculentin subfamily. {ECO:0000255}.
CC   -!- WEB RESOURCE: Name=The antimicrobial peptide database;
CC       URL="https://wangapd3.com/database/query_output.php?ID=01504";
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DR   AlphaFoldDB; P85055; -.
DR   SMR; P85055; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0002553; P:histamine secretion by mast cell; IDA:UniProtKB.
DR   GO; GO:0043306; P:positive regulation of mast cell degranulation; IDA:UniProtKB.
DR   InterPro; IPR012521; Antimicrobial_frog_2.
DR   Pfam; PF08023; Antimicrobial_2; 1.
PE   1: Evidence at protein level;
KW   Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Disulfide bond; Inflammatory response;
KW   Mast cell degranulation; Secreted.
FT   PEPTIDE         1..37
FT                   /note="Esculentin-2SE"
FT                   /evidence="ECO:0000269|PubMed:16386333"
FT                   /id="PRO_0000271246"
FT   DISULFID        31..37
FT                   /evidence="ECO:0000250|UniProtKB:P40806"
SQ   SEQUENCE   37 AA;  3840 MW;  ED1391813E4DFDC0 CRC64;
     GFFSLIKGVA KIATKGLAKN LGKMGLDLVG CKISKEC