ID   ESAA_STAA3              Reviewed;        1009 AA.
AC   A0A0H2XFP1;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   25-MAY-2022, entry version 23.
DE   RecName: Full=Type VII secretion system accessory factor EsaA {ECO:0000305};
GN   Name=esaA {ECO:0000303|PubMed:29737367}; OrderedLocusNames=SAUSA300_0279;
OS   Staphylococcus aureus (strain USA300).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=367830;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=USA300;
RX   PubMed=16517273; DOI=10.1016/s0140-6736(06)68231-7;
RA   Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G.,
RA   Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F.,
RA   Perdreau-Remington F.;
RT   "Complete genome sequence of USA300, an epidemic clone of community-
RT   acquired meticillin-resistant Staphylococcus aureus.";
RL   Lancet 367:731-739(2006).
RN   [2]
RP   FUNCTION, INTERACTION WITH ESSB, AND DISRUPTION PHENOTYPE.
RC   STRAIN=USA300;
RX   PubMed=28874412; DOI=10.1128/jb.00482-17;
RA   Aly K.A., Anderson M., Ohr R.J., Missiakas D.;
RT   "Isolation of a Membrane Protein Complex for Type VII Secretion in
RT   Staphylococcus aureus.";
RL   J. Bacteriol. 199:0-0(2017).
RN   [3]
RP   FUNCTION, AND INTERACTION WITH ESSB.
RC   STRAIN=USA300;
RX   PubMed=29737367; DOI=10.1007/s00203-018-1519-x;
RA   Ahmed M.M., Aboshanab K.M., Ragab Y.M., Missiakas D.M., Aly K.A.;
RT   "The transmembrane domain of the Staphylococcus aureus ESAT-6 component
RT   EssB mediates interaction with the integral membrane protein EsaA,
RT   facilitating partially regulated secretion in a heterologous host.";
RL   Arch. Microbiol. 200:1075-1086(2018).
CC   -!- FUNCTION: Component of the type VII secretion system (Ess). Provides
CC       together with EssB and other components such as EssC and EssE a
CC       secretion plateform accross the cytoplasmic membrane in the host.
CC       {ECO:0000269|PubMed:28874412, ECO:0000269|PubMed:29737367}.
CC   -!- SUBUNIT: Homodimer (By similarity). Interacts with EssB
CC       (PubMed:29737367, PubMed:28874412). {ECO:0000250|UniProtKB:Q2G188,
CC       ECO:0000269|PubMed:28874412, ECO:0000269|PubMed:29737367}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q2G188};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Deletion abrogates the secretion of EsxA and EsxC
CC       into the extracellular medium. {ECO:0000269|PubMed:28874412}.
CC   -!- SIMILARITY: Belongs to the EsaA family. {ECO:0000305}.
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DR   EMBL; CP000255; ABD21191.1; -; Genomic_DNA.
DR   RefSeq; WP_000728949.1; NZ_CP027476.1.
DR   AlphaFoldDB; A0A0H2XFP1; -.
DR   SMR; A0A0H2XFP1; -.
DR   EnsemblBacteria; ABD21191; ABD21191; SAUSA300_0279.
DR   KEGG; saa:SAUSA300_0279; -.
DR   HOGENOM; CLU_015018_0_0_9; -.
DR   OMA; MYNFYKP; -.
DR   Proteomes; UP000001939; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR023838; T7SS_EsaA.
DR   TIGRFAMs; TIGR03929; T7_esaA_Nterm; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Coiled coil; Membrane; Transmembrane; Transmembrane helix;
KW   Virulence.
FT   CHAIN           1..1009
FT                   /note="Type VII secretion system accessory factor EsaA"
FT                   /id="PRO_0000448088"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        822..842
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        869..889
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        903..923
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        928..948
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        979..999
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          680..707
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        680..696
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1009 AA;  114826 MW;  2F9328179F8A1792 CRC64;
     MKKKNWIYAL IVTLIIIIAI VSMIFFVQTK YGDQSEKGSQ SVSNKNNKIH IAIVNEDQPT
     TYNGKKVELG QAFIKRLANE KNYKFETVTR NVAESGLKNG GYQVMIVIPE NFSKLAMQLD
     AKTPSKISLQ YKTAVGQKEE VAKNTEKVVS NVLNDFNKNL VEIYLTSIID NLHNAQKNVG
     AIMTREHGVN SKFSNYLLNP INDFPELFTD TLVNSISANK DITKWFQTYN KSLLSANSDT
     FRVNTDYNVS TLIEKQNSLF DEHNTAMDKM LQDYKSQKDS VELDNYINAL KQMDSQIDQQ
     SSMQDTGKEE YKQTVKENLD KLREIIQSQE SPFSKGMIED YRKQLTESLQ DELANNKDLQ
     DALNSIKMNN AQFAENLEKQ LHDDIVKEPD TDTTFIYNMS KQDFIAAGLN EDEANKYEAI
     VKEAKRYKNE YNLKKPLAEH INLTDYDNQV AQDTSSLIND GVKVQRTETI KSNDINQLTV
     ATDPHFNFEG DIKINGKKYD IKDQSVQLDT SNKEYKVEVN GVAKLKKDAE KDFLKDKTMH
     LQLLFGQANR QDEPNDKKAT SVVDVTLNHN LDGRLSKDAL SQQLSALSRF DAHYKMYTDT
     KGREDKPFDN KRLIDMMVDQ VINDMESFKD DKVAVLHQID SMEENSDKLI DDILNNKKNT
     TKNKEDISKL IDQLENVKKT FAEEPQEPKI DKGKNDEFNT MSSNLDKEIS RISEKSTQLL
     SDTQESKSIA DSVSGQLNQV DNNVNKLHAT GRALGVRAND LNRQMAKNDK DNELFAKEFK
     KVLQNSKDGD RQNQALKAFM SNPVQKKNLE NVLANNGNTD VISPTLFVLL MYLLSMITAY
     IFYSYERAKG QMNFIKDDYS SKNHLWNNVI TSGVIGTTGL VEGLIVGLIA MNKFHVLAGY
     RAKFILMVIL TMMVFVLINT YLLRQVKSIG MFLMIAALGL YFVAMNNLKA AGQGVTNKIS
     PLSYIDNMFF NYLNAEHPIG LVLVILTVLV IIGFVLNMFI KHFKKERLI