ID   ESAI_PANSE              Reviewed;         210 AA.
AC   P54656;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Acyl-homoserine-lactone synthase;
DE            EC=2.3.1.184;
DE   AltName: Full=Autoinducer synthesis protein EsaI;
GN   Name=esaI;
OS   Pantoea stewartii subsp. stewartii (Erwinia stewartii).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Pantoea.
OX   NCBI_TaxID=66271;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SS104;
RX   PubMed=7665477; DOI=10.1128/jb.177.17.5000-5008.1995;
RA   von Bodman S.B., Farrand S.K.;
RT   "Capsular polysaccharide biosynthesis and pathogenicity in Erwinia
RT   stewartii require induction by an N-acylhomoserine lactone autoinducer.";
RL   J. Bacteriol. 177:5000-5008(1995).
CC   -!- FUNCTION: Required for the synthesis of OHHL (N-(3-oxohexanoyl)-L-
CC       homoserine lactone), an autoinducer molecule which binds to EsaR. OHHL
CC       is necessary for biosynthesis of EPS virulence factor (extracellular
CC       heteropolysaccharide) which plays a role in the development of
CC       Stewart's wilt on sweet corn.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a fatty acyl-[ACP] + S-adenosyl-L-methionine = an N-acyl-L-
CC         homoserine lactone + H(+) + holo-[ACP] + S-methyl-5'-thioadenosine;
CC         Xref=Rhea:RHEA:10096, Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:14125,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:55474,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:64479, ChEBI:CHEBI:138651;
CC         EC=2.3.1.184;
CC   -!- SIMILARITY: Belongs to the autoinducer synthase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00533}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L32183; AAA82096.1; -; Genomic_DNA.
DR   PIR; A57337; A57337.
DR   RefSeq; WP_006119202.1; NZ_VZPF01000009.1.
DR   PDB; 1K4J; X-ray; 2.50 A; A=1-210.
DR   PDB; 1KZF; X-ray; 1.80 A; A=1-210.
DR   PDBsum; 1K4J; -.
DR   PDBsum; 1KZF; -.
DR   AlphaFoldDB; P54656; -.
DR   SMR; P54656; -.
DR   GeneID; 61251106; -.
DR   EvolutionaryTrace; P54656; -.
DR   GO; GO:0061579; F:N-acyl homoserine lactone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-KW.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR018311; Autoind_synth_CS.
DR   InterPro; IPR001690; Autoind_synthase.
DR   PANTHER; PTHR39322; PTHR39322; 1.
DR   Pfam; PF00765; Autoind_synth; 1.
DR   PRINTS; PR01549; AUTOINDCRSYN.
DR   SUPFAM; SSF55729; SSF55729; 1.
DR   PROSITE; PS00949; AUTOINDUCER_SYNTH_1; 1.
DR   PROSITE; PS51187; AUTOINDUCER_SYNTH_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Autoinducer synthesis; Quorum sensing;
KW   S-adenosyl-L-methionine; Transferase; Virulence.
FT   CHAIN           1..210
FT                   /note="Acyl-homoserine-lactone synthase"
FT                   /id="PRO_0000210884"
FT   STRAND          2..8
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   HELIX           9..14
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   HELIX           29..39
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   STRAND          53..59
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   STRAND          62..71
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   HELIX           77..81
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   HELIX           83..86
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   STRAND          96..103
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   HELIX           105..112
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   HELIX           118..132
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   STRAND          136..143
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   HELIX           144..153
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   STRAND          158..168
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   STRAND          170..177
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   HELIX           180..194
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   HELIX           198..201
FT                   /evidence="ECO:0007829|PDB:1KZF"
FT   STRAND          202..208
FT                   /evidence="ECO:0007829|PDB:1KZF"
SQ   SEQUENCE   210 AA;  23844 MW;  7FCCF1F3709D50F5 CRC64;
     MLELFDVSYE ELQTTRSEEL YKLRKKTFSD RLGWEVICSQ GMESDEFDGP GTRYILGICE
     GQLVCSVRFT SLDRPNMITH TFQHCFSDVT LPAYGTESSR FFVDKARARA LLGEHYPISQ
     VLFLAMVNWA QNNAYGNIYT IVSRAMLKIL TRSGWQIKVI KEAFLTEKER IYLLTLPAGQ
     DDKQQLGGDV VSRTGCPPVA VTTWPLTLPV