ESF13_ARATH
ID ESF13_ARATH Reviewed; 90 AA.
AC A8MQA5;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 1.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=EMBRYO SURROUNDING FACTOR 1.3;
DE AltName: Full=Maternally expressed family protein 1.3;
DE AltName: Full=Maternally expressed gene 1.3;
DE Flags: Precursor;
GN Name=ESF1.3; Synonyms=MEG1.3; OrderedLocusNames=At1g10717;
GN ORFNames=F20B24, T16B5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP STRUCTURE BY NMR OF 23-90, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF CYS-41; CYS-46; CYS-56;
RP TRP-57; CYS-58; CYS-59; CYS-69; TRP-70; CYS-82 AND CYS-86.
RC STRAIN=cv. Columbia;
RX PubMed=24723605; DOI=10.1126/science.1243005;
RA Costa L.M., Marshall E., Tesfaye M., Silverstein K.A., Mori M., Umetsu Y.,
RA Otterbach S.L., Papareddy R., Dickinson H.G., Boutiller K.,
RA VandenBosch K.A., Ohki S., Gutierrez-Marcos J.F.;
RT "Central cell-derived peptides regulate early embryo patterning in
RT flowering plants.";
RL Science 344:168-172(2014).
CC -!- FUNCTION: Maternally-contributed central cell peptide regulating
CC suspensor development and correct auxin distribution in early
CC developing embryos. {ECO:0000269|PubMed:24723605}.
CC -!- TISSUE SPECIFICITY: Expressed exclusively in ovule embryo sacs and in
CC early developing endosperms. {ECO:0000269|PubMed:24723605}.
CC -!- DEVELOPMENTAL STAGE: Primarily expressed in the central cell gamete of
CC nonfertilized ovules, which upon fertilization gives rise to the
CC endosperm, and later in the micropylar endosperm, which surrounds the
CC embryo. {ECO:0000269|PubMed:24723605}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, due to redundancy with
CC ESF1.1 and ESF1.2. Simultaneous down-regulation of all 3 genes by RNAi
CC induces embryo abnormalities. {ECO:0000269|PubMed:24723605}.
CC -!- SIMILARITY: Belongs to the MEG family. {ECO:0000305}.
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DR EMBL; AC007354; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC009398; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE28633.1; -; Genomic_DNA.
DR RefSeq; NP_001077509.1; NM_001084040.1.
DR PDB; 2RU1; NMR; -; A=23-90.
DR PDBsum; 2RU1; -.
DR AlphaFoldDB; A8MQA5; -.
DR BMRB; A8MQA5; -.
DR SMR; A8MQA5; -.
DR STRING; 3702.AT1G10717.1; -.
DR PaxDb; A8MQA5; -.
DR PRIDE; A8MQA5; -.
DR EnsemblPlants; AT1G10717.1; AT1G10717.1; AT1G10717.
DR GeneID; 5007678; -.
DR Gramene; AT1G10717.1; AT1G10717.1; AT1G10717.
DR KEGG; ath:AT1G10717; -.
DR Araport; AT1G10717; -.
DR TAIR; locus:4010713427; AT1G10717.
DR HOGENOM; CLU_183999_1_0_1; -.
DR OMA; SHTTLIC; -.
DR OrthoDB; 1627959at2759; -.
DR PhylomeDB; A8MQA5; -.
DR PRO; PR:A8MQA5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; A8MQA5; baseline.
DR GO; GO:0000578; P:embryonic axis specification; IMP:UniProtKB.
DR GO; GO:0010098; P:suspensor development; IMP:UniProtKB.
DR InterPro; IPR041608; ESF1_brassicaceae.
DR Pfam; PF18209; ESF1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Developmental protein; Disulfide bond; Reference proteome;
KW Signal.
FT SIGNAL 1..22
FT CHAIN 23..90
FT /note="EMBRYO SURROUNDING FACTOR 1.3"
FT /id="PRO_0000430061"
FT DISULFID 41..58
FT DISULFID 46..86
FT DISULFID 56..82
FT DISULFID 59..69
FT MUTAGEN 41
FT /note="Missing: Disrupted topology and loss of activity;
FT when associated with C-58-DEL."
FT /evidence="ECO:0000269|PubMed:24723605"
FT MUTAGEN 46
FT /note="Missing: Disrupted topology and loss of activity;
FT when associated with C-86-DEL."
FT /evidence="ECO:0000269|PubMed:24723605"
FT MUTAGEN 56
FT /note="Missing: Disrupted topology and loss of activity;
FT when associated with C-82-DEL."
FT /evidence="ECO:0000269|PubMed:24723605"
FT MUTAGEN 57
FT /note="W->A: No effect on topology, but loss of activity;
FT when associated with A-70."
FT /evidence="ECO:0000269|PubMed:24723605"
FT MUTAGEN 58
FT /note="Missing: Disrupted topology and loss of activity;
FT when associated with C-41-DEL."
FT /evidence="ECO:0000269|PubMed:24723605"
FT MUTAGEN 59
FT /note="Missing: Disrupted topology and loss of activity;
FT when associated with C-69-DEL."
FT /evidence="ECO:0000269|PubMed:24723605"
FT MUTAGEN 69
FT /note="Missing: Disrupted topology and loss of activity;
FT when associated with C-59-DEL."
FT /evidence="ECO:0000269|PubMed:24723605"
FT MUTAGEN 70
FT /note="W->A: No effect on topology, but loss of activity;
FT when associated with A-57."
FT /evidence="ECO:0000269|PubMed:24723605"
FT MUTAGEN 82
FT /note="Missing: Disrupted topology and loss of activity;
FT when associated with C-56-DEL."
FT /evidence="ECO:0000269|PubMed:24723605"
FT MUTAGEN 86
FT /note="Missing: Disrupted topology and loss of activity;
FT when associated with C-46-DEL."
FT /evidence="ECO:0000269|PubMed:24723605"
FT STRAND 40..42
FT /evidence="ECO:0007829|PDB:2RU1"
FT STRAND 45..47
FT /evidence="ECO:0007829|PDB:2RU1"
FT STRAND 49..51
FT /evidence="ECO:0007829|PDB:2RU1"
FT STRAND 63..65
FT /evidence="ECO:0007829|PDB:2RU1"
FT TURN 72..74
FT /evidence="ECO:0007829|PDB:2RU1"
FT HELIX 76..84
FT /evidence="ECO:0007829|PDB:2RU1"
SQ SEQUENCE 90 AA; 10283 MW; 5C976A62A591FD47 CRC64;
MKSSHTTLIC IIMLSLFALH ECEKMEVKEI GRSSKIILPA CMHETCSGGF SLKNDCWCCL
RLKTKQARCW KEKEFPNAKE LCFANCPPLE
