ESM2_DROME
ID ESM2_DROME Reviewed; 218 AA.
AC O97177; Q5S3Y0; Q5S3Y1; Q5S3Y3; Q5S456; Q5S480; Q5S4A4; Q5S4B6; Q5S4H6;
AC Q5S4K0;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Enhancer of split M2 protein {ECO:0000303|PubMed:10072784};
DE Short=E(spl)m2 {ECO:0000303|PubMed:10072784};
GN Name=E(spl)m2-BFM {ECO:0000312|FlyBase:FBgn0002592};
GN Synonyms=m2 {ECO:0000303|PubMed:10072784};
GN ORFNames=CG6104 {ECO:0000312|FlyBase:FBgn0002592};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=10072784; DOI=10.1016/s0925-4773(98)00212-3;
RA Wurmbach E., Wech I., Preiss A.;
RT "The Enhancer of split complex of Drosophila melanogaster harbors three
RT classes of Notch responsive genes.";
RL Mech. Dev. 80:171-180(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS THR-12; ALA-21 DEL; CYS-47;
RP GLN-GLN-GLN-GLN-66 INS; 150-ILE--SER-153 DEL; 150-ILE--ASN-158 DEL; GLU-212
RP AND MET-213.
RC STRAIN=Madang_F12, Madang_F16, Madang_F18, Madang_F21, Madang_F24,
RC Madang_F30, Madang_F4, Madang_F9, Madang_M10, Madang_M17, Madang_M22,
RC Madang_M27, Madang_M3, Madang_M4, Madang_M9, NVIII-1, NVIII-18, NVIII-2,
RC NVIII-22, NVIII-24, NVIII-28, NVIII-41, NVIII-42, NVIII-46, NVIII-5,
RC NVIII-9, NVIII-m11, NVIII-m12, NVIII-m13, NVIII-m15, and NVIII-m19;
RX PubMed=15537803; DOI=10.1093/molbev/msi046;
RA Macdonald S.J., Long A.D.;
RT "Identifying signatures of selection at the enhancer of split neurogenic
RT gene complex in Drosophila.";
RL Mol. Biol. Evol. 22:607-619(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Part of the Notch signaling pathway.
CC {ECO:0000269|PubMed:10072784}.
CC -!- DEVELOPMENTAL STAGE: Expressed at the time when separation of neural
CC and epidermal precursors cells occurs. Detected in the neuro-ectoderm
CC of stage 9 embryos. At stage 10/11, accumulates at high levels in the
CC presumptive mesoderm, however, it disappears quickly with the onset of
CC germ band retraction. In eye disk, expression occurs close to, as well
CC as posterior to the morphogenetic furrow. In the wing disk, found in
CC the proneural clusters areas, the dorso-ventral boundary and
CC vein/intervein regions. {ECO:0000269|PubMed:10072784}.
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DR EMBL; AJ010168; CAB39174.1; -; Genomic_DNA.
DR EMBL; AY779906; AAV59054.1; -; Genomic_DNA.
DR EMBL; AY779907; AAV59066.1; -; Genomic_DNA.
DR EMBL; AY779908; AAV59078.1; -; Genomic_DNA.
DR EMBL; AY779909; AAV59090.1; -; Genomic_DNA.
DR EMBL; AY779910; AAV59102.1; -; Genomic_DNA.
DR EMBL; AY779911; AAV59114.1; -; Genomic_DNA.
DR EMBL; AY779912; AAV59126.1; -; Genomic_DNA.
DR EMBL; AY779913; AAV59138.1; -; Genomic_DNA.
DR EMBL; AY779914; AAV59150.1; -; Genomic_DNA.
DR EMBL; AY779915; AAV59162.1; -; Genomic_DNA.
DR EMBL; AY779916; AAV59174.1; -; Genomic_DNA.
DR EMBL; AY779917; AAV59186.1; -; Genomic_DNA.
DR EMBL; AY779918; AAV59198.1; -; Genomic_DNA.
DR EMBL; AY779919; AAV59210.1; -; Genomic_DNA.
DR EMBL; AY779920; AAV59222.1; -; Genomic_DNA.
DR EMBL; AY779921; AAV59234.1; -; Genomic_DNA.
DR EMBL; AY779933; AAV59030.1; -; Genomic_DNA.
DR EMBL; AY779934; AAV59031.1; -; Genomic_DNA.
DR EMBL; AY779935; AAV59032.1; -; Genomic_DNA.
DR EMBL; AY779936; AAV59033.1; -; Genomic_DNA.
DR EMBL; AY779937; AAV59034.1; -; Genomic_DNA.
DR EMBL; AY779938; AAV59035.1; -; Genomic_DNA.
DR EMBL; AY779939; AAV59036.1; -; Genomic_DNA.
DR EMBL; AY779940; AAV59037.1; -; Genomic_DNA.
DR EMBL; AY779941; AAV59038.1; -; Genomic_DNA.
DR EMBL; AY779942; AAV59039.1; -; Genomic_DNA.
DR EMBL; AY779943; AAV59040.1; -; Genomic_DNA.
DR EMBL; AY779944; AAV59041.1; -; Genomic_DNA.
DR EMBL; AY779945; AAV59042.1; -; Genomic_DNA.
DR EMBL; AY779946; AAV59043.1; -; Genomic_DNA.
DR EMBL; AY779947; AAV59044.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF56549.1; -; Genomic_DNA.
DR EMBL; AY071096; AAL48718.1; -; mRNA.
DR RefSeq; NP_524508.1; NM_079784.3.
DR AlphaFoldDB; O97177; -.
DR STRING; 7227.FBpp0084354; -.
DR PaxDb; O97177; -.
DR PRIDE; O97177; -.
DR DNASU; 43155; -.
DR EnsemblMetazoa; FBtr0084981; FBpp0084354; FBgn0002592.
DR GeneID; 43155; -.
DR KEGG; dme:Dmel_CG6104; -.
DR CTD; 43155; -.
DR FlyBase; FBgn0002592; E(spl)m2-BFM.
DR VEuPathDB; VectorBase:FBgn0002592; -.
DR eggNOG; ENOG502T8HW; Eukaryota.
DR HOGENOM; CLU_1195934_0_0_1; -.
DR InParanoid; O97177; -.
DR OMA; HGRNCQH; -.
DR OrthoDB; 1601113at2759; -.
DR PhylomeDB; O97177; -.
DR BioGRID-ORCS; 43155; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43155; -.
DR PRO; PR:O97177; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0002592; Expressed in eye disc (Drosophila) and 46 other tissues.
DR Genevisible; O97177; DM.
DR GO; GO:0001708; P:cell fate specification; IMP:FlyBase.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0045747; P:positive regulation of Notch signaling pathway; IMP:FlyBase.
DR GO; GO:0007423; P:sensory organ development; IMP:FlyBase.
DR InterPro; IPR029686; Malpha/m4/m2.
DR Pfam; PF15952; ESM4; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; Neurogenesis;
KW Notch signaling pathway; Reference proteome.
FT CHAIN 1..218
FT /note="Enhancer of split M2 protein"
FT /id="PRO_0000087058"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 64..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 137..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..79
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 144..164
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 12
FT /note="S -> T (in strain: NVIII-41)"
FT /evidence="ECO:0000269|PubMed:15537803"
FT VARIANT 21
FT /note="Missing (in strain: NVIII-m11)"
FT /evidence="ECO:0000269|PubMed:15537803"
FT VARIANT 47
FT /note="G -> C (in strain: NVIII-9)"
FT /evidence="ECO:0000269|PubMed:15537803"
FT VARIANT 65
FT /note="T -> TQQQQ (in strain: NVIII-m11)"
FT VARIANT 150..158
FT /note="Missing (in strain: NVIII-42 and NVIII-18)"
FT /evidence="ECO:0000269|PubMed:15537803"
FT VARIANT 150..153
FT /note="Missing (in strain: Madang_F12 and Madang_M22)"
FT /evidence="ECO:0000269|PubMed:15537803"
FT VARIANT 212
FT /note="Q -> E (in strain: NVIII-m13)"
FT /evidence="ECO:0000269|PubMed:15537803"
FT VARIANT 213
FT /note="L -> M (in strain: NVIII-m11)"
FT /evidence="ECO:0000269|PubMed:15537803"
SQ SEQUENCE 218 AA; 24118 MW; 8DC97662E9B0BE13 CRC64;
MYLDTKNLTA SSTSALTAAT ASNSKSTRRM RNVWKPLSRL LKVGGKGRTA QATLAHPNNV
DLNNTQQQQQ QQQQELLIED DTKSTPEESI HKYGLELRQL PEEEQVSLTQ PPNTPTLISS
VHVDKVSAQS CGNCQHGRNC QHRHHSSSNI NSSSSSSNMN SSSATQLPSN LWDLQLPLQY
ISTDNGTFFW ANTQDRVDDD LLHALLCQSF SQLPGTLC
