ESM4_DROME
ID ESM4_DROME Reviewed; 152 AA.
AC P13095; Q56L08; Q56L11; Q56L12; Q56L19; Q56L29; Q56L35; Q56L44; Q56L45;
AC Q56L64; Q56L67; Q56L68; Q56L69; Q56L92; Q5S478; Q5S4B4; Q5S4J8; Q9VBI9;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Enhancer of split m4 protein {ECO:0000303|PubMed:2540957};
DE Short=E(spl)m4 {ECO:0000303|PubMed:2540957};
GN Name=E(spl)m4-BFM {ECO:0000312|FlyBase:FBgn0002629};
GN Synonyms=m4 {ECO:0000303|PubMed:2540957};
GN ORFNames=CG6099 {ECO:0000312|FlyBase:FBgn0002629};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2540957; DOI=10.1002/j.1460-2075.1989.tb03365.x;
RA Klaembt C., Knust E., Tietze K., Campos-Ortega J.A.;
RT "Closely related transcripts encoded by the neurogenic gene complex
RT enhancer of split of Drosophila melanogaster.";
RL EMBO J. 8:203-210(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS ALA-11 AND LYS-82.
RC STRAIN=NVIII-1, NVIII-18, NVIII-2, NVIII-22, NVIII-24, NVIII-28, NVIII-41,
RC NVIII-42, NVIII-46, NVIII-5, NVIII-9, NVIII-m11, NVIII-m12, NVIII-m13,
RC NVIII-m15, and NVIII-m19;
RX PubMed=15537803; DOI=10.1093/molbev/msi046;
RA Macdonald S.J., Long A.D.;
RT "Identifying signatures of selection at the enhancer of split neurogenic
RT gene complex in Drosophila.";
RL Mol. Biol. Evol. 22:607-619(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-125, AND VARIANTS ALA-11 AND PRO-63.
RC STRAIN=Nv2001_m0564, Nv2001_m0565, Nv2001_m0566, Nv2001_m0567,
RC Nv2001_m0568, Nv2001_m0569, Nv2001_m0570, Nv2001_m0571, Nv2001_m0572,
RC Nv2001_m0573, Nv2001_m0574, Nv2001_m0575, Nv2001_m0576, Nv2001_m0577,
RC Nv2001_m0578, Nv2001_m0579, Nv2001_m0580, Nv2001_m0581, Nv2001_m0582,
RC Nv2001_m0583, Nv2001_m0584, Nv2001_m0585, Nv2001_m0586, Nv2001_m0587,
RC Nv2001_m0588, Nv2001_m0589, Nv2001_m0590, Nv2001_m0591, Nv2001_m0592,
RC Nv2001_m0593, Nv2001_m0594, Nv2001_m0595, Nv2001_m0596, Nv2001_m0597,
RC Nv2001_m0598, Nv2001_m0599, Nv2001_m0600, Nv2001_m0601, Nv2001_m0602,
RC Nv2001_m0603, Nv2001_m0604, Nv2001_m0605, Nv2001_m0606, Nv2001_m0607,
RC Nv2001_m0608, Nv2001_m0609, Nv2001_m0610, Nv2001_m0611, Nv2001_m0612,
RC Nv2001_m0613, Nv2001_m0614, Nv2001_m0615, Nv2001_m0616, Nv2001_m0617,
RC Nv2001_m0618, Nv2001_m0619, Nv2001_m0620, Nv2001_m0622, Nv2001_m0623,
RC Nv2001_m0624, Nv2001_m0625, Nv2001_m0626, Nv2001_m0627, Nv2001_m0628,
RC Nv2001_m0629, Nv2001_m0630, Nv2001_m0631, Nv2001_m0632, Nv2001_m0633,
RC Nv2001_m0634, Nv2001_m0635, Nv2001_m0636, Nv2001_m0637, Nv2001_m0638,
RC Nv2001_m0639, Nv2001_m0640, Nv2001_m0641, Nv2001_m0642, Nv2001_m0643,
RC Nv2001_m0644, Nv2001_m0645, Nv2001_m0646, Nv2001_m0647, Nv2001_m0648,
RC Nv2001_m0649, Nv2001_m0650, Nv2001_m0651, Nv2001_m0652, Nv2001_m0653,
RC Nv2001_m0654, Nv2001_m0655, Nv2001_m0656, and Nv2001_m0657;
RX PubMed=16356268; DOI=10.1186/gb-2005-6-12-r105;
RA Macdonald S.J., Pastinen T., Genissel A., Cornforth T.W., Long A.D.;
RT "A low-cost open-source SNP genotyping platform for association mapping
RT applications.";
RL Genome Biol. 6:R105.1-R105.11(2005).
CC -!- FUNCTION: Part of the Notch signaling pathway.
CC -!- DEVELOPMENTAL STAGE: Expressed at the time when separation of neural
CC and epidermal precursors cells occurs. Mesectodermal expression appears
CC shortly before the onset of gastrulation. In imaginal disks, expression
CC is seen primarily within presumptive proneural clusters of eye-
CC antennal, wing and leg disks.
CC -!- SIMILARITY: Belongs to the M4-like protein family. {ECO:0000305}.
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DR EMBL; X16551; CAA34551.1; -; Genomic_DNA.
DR EMBL; AY779906; AAV59055.1; -; Genomic_DNA.
DR EMBL; AY779907; AAV59067.1; -; Genomic_DNA.
DR EMBL; AY779908; AAV59079.1; -; Genomic_DNA.
DR EMBL; AY779909; AAV59091.1; -; Genomic_DNA.
DR EMBL; AY779910; AAV59103.1; -; Genomic_DNA.
DR EMBL; AY779911; AAV59115.1; -; Genomic_DNA.
DR EMBL; AY779912; AAV59127.1; -; Genomic_DNA.
DR EMBL; AY779913; AAV59139.1; -; Genomic_DNA.
DR EMBL; AY779914; AAV59151.1; -; Genomic_DNA.
DR EMBL; AY779915; AAV59163.1; -; Genomic_DNA.
DR EMBL; AY779916; AAV59175.1; -; Genomic_DNA.
DR EMBL; AY779917; AAV59187.1; -; Genomic_DNA.
DR EMBL; AY779918; AAV59199.1; -; Genomic_DNA.
DR EMBL; AY779919; AAV59211.1; -; Genomic_DNA.
DR EMBL; AY779920; AAV59223.1; -; Genomic_DNA.
DR EMBL; AY779921; AAV59235.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF56551.1; -; Genomic_DNA.
DR EMBL; AY906350; AAX60696.1; -; Genomic_DNA.
DR EMBL; AY906351; AAX60697.1; -; Genomic_DNA.
DR EMBL; AY906352; AAX60698.1; -; Genomic_DNA.
DR EMBL; AY906353; AAX60699.1; -; Genomic_DNA.
DR EMBL; AY906354; AAX60700.1; -; Genomic_DNA.
DR EMBL; AY906355; AAX60701.1; -; Genomic_DNA.
DR EMBL; AY906356; AAX60702.1; -; Genomic_DNA.
DR EMBL; AY906357; AAX60703.1; -; Genomic_DNA.
DR EMBL; AY906358; AAX60704.1; -; Genomic_DNA.
DR EMBL; AY906359; AAX60705.1; -; Genomic_DNA.
DR EMBL; AY906360; AAX60706.1; -; Genomic_DNA.
DR EMBL; AY906361; AAX60707.1; -; Genomic_DNA.
DR EMBL; AY906362; AAX60708.1; -; Genomic_DNA.
DR EMBL; AY906363; AAX60709.1; -; Genomic_DNA.
DR EMBL; AY906364; AAX60710.1; -; Genomic_DNA.
DR EMBL; AY906365; AAX60711.1; -; Genomic_DNA.
DR EMBL; AY906366; AAX60712.1; -; Genomic_DNA.
DR EMBL; AY906367; AAX60713.1; -; Genomic_DNA.
DR EMBL; AY906368; AAX60714.1; -; Genomic_DNA.
DR EMBL; AY906369; AAX60715.1; -; Genomic_DNA.
DR EMBL; AY906370; AAX60716.1; -; Genomic_DNA.
DR EMBL; AY906371; AAX60717.1; -; Genomic_DNA.
DR EMBL; AY906372; AAX60718.1; -; Genomic_DNA.
DR EMBL; AY906373; AAX60719.1; -; Genomic_DNA.
DR EMBL; AY906374; AAX60720.1; -; Genomic_DNA.
DR EMBL; AY906375; AAX60721.1; -; Genomic_DNA.
DR EMBL; AY906376; AAX60722.1; -; Genomic_DNA.
DR EMBL; AY906377; AAX60723.1; -; Genomic_DNA.
DR EMBL; AY906378; AAX60724.1; -; Genomic_DNA.
DR EMBL; AY906379; AAX60725.1; -; Genomic_DNA.
DR EMBL; AY906380; AAX60726.1; -; Genomic_DNA.
DR EMBL; AY906381; AAX60727.1; -; Genomic_DNA.
DR EMBL; AY906382; AAX60728.1; -; Genomic_DNA.
DR EMBL; AY906383; AAX60729.1; -; Genomic_DNA.
DR EMBL; AY906384; AAX60730.1; -; Genomic_DNA.
DR EMBL; AY906385; AAX60731.1; -; Genomic_DNA.
DR EMBL; AY906386; AAX60732.1; -; Genomic_DNA.
DR EMBL; AY906387; AAX60733.1; -; Genomic_DNA.
DR EMBL; AY906388; AAX60734.1; -; Genomic_DNA.
DR EMBL; AY906389; AAX60735.1; -; Genomic_DNA.
DR EMBL; AY906390; AAX60736.1; -; Genomic_DNA.
DR EMBL; AY906391; AAX60737.1; -; Genomic_DNA.
DR EMBL; AY906392; AAX60738.1; -; Genomic_DNA.
DR EMBL; AY906393; AAX60739.1; -; Genomic_DNA.
DR EMBL; AY906394; AAX60740.1; -; Genomic_DNA.
DR EMBL; AY906395; AAX60741.1; -; Genomic_DNA.
DR EMBL; AY906396; AAX60742.1; -; Genomic_DNA.
DR EMBL; AY906397; AAX60743.1; -; Genomic_DNA.
DR EMBL; AY906398; AAX60744.1; -; Genomic_DNA.
DR EMBL; AY906399; AAX60745.1; -; Genomic_DNA.
DR EMBL; AY906400; AAX60746.1; -; Genomic_DNA.
DR EMBL; AY906401; AAX60747.1; -; Genomic_DNA.
DR EMBL; AY906402; AAX60748.1; -; Genomic_DNA.
DR EMBL; AY906403; AAX60749.1; -; Genomic_DNA.
DR EMBL; AY906404; AAX60750.1; -; Genomic_DNA.
DR EMBL; AY906405; AAX60751.1; -; Genomic_DNA.
DR EMBL; AY906406; AAX60752.1; -; Genomic_DNA.
DR EMBL; AY906407; AAX60753.1; -; Genomic_DNA.
DR EMBL; AY906408; AAX60754.1; -; Genomic_DNA.
DR EMBL; AY906409; AAX60755.1; -; Genomic_DNA.
DR EMBL; AY906410; AAX60756.1; -; Genomic_DNA.
DR EMBL; AY906411; AAX60757.1; -; Genomic_DNA.
DR EMBL; AY906412; AAX60758.1; -; Genomic_DNA.
DR EMBL; AY906413; AAX60759.1; -; Genomic_DNA.
DR EMBL; AY906414; AAX60760.1; -; Genomic_DNA.
DR EMBL; AY906415; AAX60761.1; -; Genomic_DNA.
DR EMBL; AY906416; AAX60762.1; -; Genomic_DNA.
DR EMBL; AY906417; AAX60763.1; -; Genomic_DNA.
DR EMBL; AY906418; AAX60764.1; -; Genomic_DNA.
DR EMBL; AY906419; AAX60765.1; -; Genomic_DNA.
DR EMBL; AY906420; AAX60766.1; -; Genomic_DNA.
DR EMBL; AY906421; AAX60767.1; -; Genomic_DNA.
DR EMBL; AY906422; AAX60768.1; -; Genomic_DNA.
DR EMBL; AY906423; AAX60769.1; -; Genomic_DNA.
DR EMBL; AY906424; AAX60770.1; -; Genomic_DNA.
DR EMBL; AY906425; AAX60771.1; -; Genomic_DNA.
DR EMBL; AY906426; AAX60772.1; -; Genomic_DNA.
DR EMBL; AY906427; AAX60773.1; -; Genomic_DNA.
DR EMBL; AY906428; AAX60774.1; -; Genomic_DNA.
DR EMBL; AY906429; AAX60775.1; -; Genomic_DNA.
DR EMBL; AY906430; AAX60776.1; -; Genomic_DNA.
DR EMBL; AY906431; AAX60777.1; -; Genomic_DNA.
DR EMBL; AY906432; AAX60778.1; -; Genomic_DNA.
DR EMBL; AY906433; AAX60779.1; -; Genomic_DNA.
DR EMBL; AY906434; AAX60780.1; -; Genomic_DNA.
DR EMBL; AY906435; AAX60781.1; -; Genomic_DNA.
DR EMBL; AY906436; AAX60782.1; -; Genomic_DNA.
DR EMBL; AY906437; AAX60783.1; -; Genomic_DNA.
DR EMBL; AY906438; AAX60784.1; -; Genomic_DNA.
DR EMBL; AY906439; AAX60785.1; -; Genomic_DNA.
DR EMBL; AY906440; AAX60786.1; -; Genomic_DNA.
DR EMBL; AY906441; AAX60787.1; -; Genomic_DNA.
DR EMBL; AY906442; AAX60788.1; -; Genomic_DNA.
DR PIR; S03630; S03630.
DR RefSeq; NP_524510.1; NM_079786.3.
DR AlphaFoldDB; P13095; -.
DR BioGRID; 68057; 9.
DR DIP; DIP-23216N; -.
DR IntAct; P13095; 2.
DR STRING; 7227.FBpp0084353; -.
DR PaxDb; P13095; -.
DR DNASU; 43157; -.
DR EnsemblMetazoa; FBtr0084980; FBpp0084353; FBgn0002629.
DR GeneID; 43157; -.
DR KEGG; dme:Dmel_CG6099; -.
DR CTD; 43157; -.
DR FlyBase; FBgn0002629; E(spl)m4-BFM.
DR VEuPathDB; VectorBase:FBgn0002629; -.
DR eggNOG; ENOG502T7ZT; Eukaryota.
DR GeneTree; ENSGT00900000142304; -.
DR HOGENOM; CLU_1688576_0_0_1; -.
DR InParanoid; P13095; -.
DR OMA; EDYEFEQ; -.
DR OrthoDB; 1480488at2759; -.
DR PhylomeDB; P13095; -.
DR SignaLink; P13095; -.
DR BioGRID-ORCS; 43157; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43157; -.
DR PRO; PR:P13095; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0002629; Expressed in ventral ectoderm and 19 other tissues.
DR ExpressionAtlas; P13095; baseline and differential.
DR Genevisible; P13095; DM.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; IPI:FlyBase.
DR GO; GO:0001708; P:cell fate specification; IMP:FlyBase.
DR GO; GO:0045746; P:negative regulation of Notch signaling pathway; IMP:FlyBase.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0007423; P:sensory organ development; IMP:FlyBase.
DR InterPro; IPR029686; Malpha/m4/m2.
DR PANTHER; PTHR12254; PTHR12254; 1.
DR Pfam; PF15952; ESM4; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Developmental protein; Differentiation; Neurogenesis;
KW Notch signaling pathway; Reference proteome.
FT CHAIN 1..152
FT /note="Enhancer of split m4 protein"
FT /id="PRO_0000087059"
FT COILED 23..61
FT /evidence="ECO:0000255"
FT VARIANT 11
FT /note="T -> A (in strain: Nv2001_m0595 and NVIII-m11)"
FT /evidence="ECO:0000269|PubMed:15537803,
FT ECO:0000269|PubMed:16356268"
FT VARIANT 63
FT /note="R -> P (in strain: Nv2001_m0592)"
FT /evidence="ECO:0000269|PubMed:16356268"
FT VARIANT 82
FT /note="E -> K (in strain: NVIII-41)"
FT /evidence="ECO:0000269|PubMed:15537803"
SQ SEQUENCE 152 AA; 17250 MW; D7F642838BD2DA0C CRC64;
MCQNKINTNN TMTIKSNKKL SYSVKKLLQK IFKQQQRVEE EQNLKNALKA NSLESLESME
NSRNADLESA SICASLESCE NEANERLSQS CEIEDYDFEQ LPTVPVHFVR TAHGTFFWTA
VSDLPADNDL VEPLYCSTSN AIAIPQDRWV QA
