ESM7_DROME
ID ESM7_DROME Reviewed; 186 AA.
AC P13097; Q5S487; Q5S4J5; Q9VBI6;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 03-AUG-2022, entry version 192.
DE RecName: Full=Enhancer of split m7 protein;
DE Short=E(spl)m7;
GN Name=E(spl)m7-HLH {ECO:0000312|FlyBase:FBgn0002633};
GN Synonyms=HLHm7 {ECO:0000312|FlyBase:FBgn0002633};
GN ORFNames=CG8361 {ECO:0000312|FlyBase:FBgn0002633};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RX PubMed=2540957; DOI=10.1002/j.1460-2075.1989.tb03365.x;
RA Klaembt C., Knust E., Tietze K., Campos-Ortega J.A.;
RT "Closely related transcripts encoded by the neurogenic gene complex
RT enhancer of split of Drosophila melanogaster.";
RL EMBO J. 8:203-210(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT 155-SER--CYS-156 DEL.
RC STRAIN=NVIII-1, NVIII-18, NVIII-2, NVIII-22, NVIII-24, NVIII-28, NVIII-41,
RC NVIII-42, NVIII-46, NVIII-5, NVIII-9, NVIII-m11, NVIII-m12, NVIII-m13,
RC NVIII-m15, and NVIII-m19;
RX PubMed=15537803; DOI=10.1093/molbev/msi046;
RA Macdonald S.J., Long A.D.;
RT "Identifying signatures of selection at the enhancer of split neurogenic
RT gene complex in Drosophila.";
RL Mol. Biol. Evol. 22:607-619(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP DOMAIN WRPW MOTIF.
RX PubMed=8001118; DOI=10.1016/0092-8674(94)90070-1;
RA Paroush Z., Finley R.L. Jr., Kidd T., Wainwright S.M., Ingham P.W.,
RA Brent R., Ish-Horowicz D.;
RT "Groucho is required for Drosophila neurogenesis, segmentation, and sex
RT determination and interacts directly with hairy-related bHLH proteins.";
RL Cell 79:805-815(1994).
RN [7]
RP DEVELOPMENTAL STAGE.
RX PubMed=22357926; DOI=10.1242/dev.071779;
RA Zacharioudaki E., Magadi S.S., Delidakis C.;
RT "bHLH-O proteins are crucial for Drosophila neuroblast self-renewal and
RT mediate Notch-induced overproliferation.";
RL Development 139:1258-1269(2012).
CC -!- FUNCTION: Participates in the control of cell fate choice by
CC uncommitted neuroectodermal cells in the embryo. Transcriptional
CC repressor. Binds DNA on N-box motifs: 5'-CACNAG-3'.
CC -!- SUBUNIT: Transcription repression requires formation of a complex with
CC a corepressor protein (Groucho). Forms homodimers.
CC {ECO:0000250|UniProtKB:P14003, ECO:0000250|UniProtKB:Q26263}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: In larva, detected in the optic lobe and weakly in
CC the neuroblasts (at protein level) (PubMed:22357926). Expressed at the
CC time when separation of neural and epidermal precursor cells occurs
CC (PubMed:2540957). Mesectodermal expression appears shortly before the
CC onset of gastrulation (PubMed:2540957). In imaginal disks, expression
CC is seen primarily within presumptive proneural clusters of eye-
CC antennal, wing and leg disks (PubMed:2540957).
CC {ECO:0000269|PubMed:22357926, ECO:0000269|PubMed:2540957}.
CC -!- DOMAIN: The orange domain and the basic helix-loop-helix motif mediate
CC repression of specific transcriptional activators, such as basic helix-
CC loop-helix protein dimers. {ECO:0000269|PubMed:8001118}.
CC -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC domain necessary for the interaction with Groucho, a transcriptional
CC corepressor recruited to specific target DNA by Hairy-related proteins.
CC {ECO:0000269|PubMed:8001118}.
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DR EMBL; X16553; CAA34553.1; -; Genomic_DNA.
DR EMBL; AY779906; AAV59050.1; -; Genomic_DNA.
DR EMBL; AY779907; AAV59062.1; -; Genomic_DNA.
DR EMBL; AY779908; AAV59074.1; -; Genomic_DNA.
DR EMBL; AY779909; AAV59086.1; -; Genomic_DNA.
DR EMBL; AY779910; AAV59098.1; -; Genomic_DNA.
DR EMBL; AY779911; AAV59110.1; -; Genomic_DNA.
DR EMBL; AY779912; AAV59122.1; -; Genomic_DNA.
DR EMBL; AY779913; AAV59134.1; -; Genomic_DNA.
DR EMBL; AY779914; AAV59146.1; -; Genomic_DNA.
DR EMBL; AY779915; AAV59158.1; -; Genomic_DNA.
DR EMBL; AY779916; AAV59170.1; -; Genomic_DNA.
DR EMBL; AY779917; AAV59182.1; -; Genomic_DNA.
DR EMBL; AY779918; AAV59194.1; -; Genomic_DNA.
DR EMBL; AY779919; AAV59206.1; -; Genomic_DNA.
DR EMBL; AY779920; AAV59218.1; -; Genomic_DNA.
DR EMBL; AY779921; AAV59230.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF56554.1; -; Genomic_DNA.
DR EMBL; BT022121; AAY51516.1; -; mRNA.
DR PIR; S03626; S03626.
DR RefSeq; NP_536753.1; NM_080505.3.
DR AlphaFoldDB; P13097; -.
DR SMR; P13097; -.
DR BioGRID; 68060; 33.
DR DIP; DIP-626N; -.
DR ELM; P13097; -.
DR IntAct; P13097; 13.
DR MINT; P13097; -.
DR STRING; 7227.FBpp0084334; -.
DR PaxDb; P13097; -.
DR EnsemblMetazoa; FBtr0084960; FBpp0084334; FBgn0002633.
DR GeneID; 43160; -.
DR KEGG; dme:Dmel_CG8361; -.
DR CTD; 43160; -.
DR FlyBase; FBgn0002633; E(spl)m7-HLH.
DR VEuPathDB; VectorBase:FBgn0002633; -.
DR eggNOG; KOG4304; Eukaryota.
DR GeneTree; ENSGT00940000167178; -.
DR HOGENOM; CLU_068550_2_2_1; -.
DR InParanoid; P13097; -.
DR OMA; FGTHLMT; -.
DR OrthoDB; 1483774at2759; -.
DR PhylomeDB; P13097; -.
DR SignaLink; P13097; -.
DR BioGRID-ORCS; 43160; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43160; -.
DR PRO; PR:P13097; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0002633; Expressed in embryonic optic lobe primordium and 51 other tissues.
DR Genevisible; P13097; DM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:UniProtKB.
DR GO; GO:0008134; F:transcription factor binding; IGI:FlyBase.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR003650; Orange_dom.
DR Pfam; PF07527; Hairy_orange; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00511; ORANGE; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS51054; ORANGE; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..186
FT /note="Enhancer of split m7 protein"
FT /id="PRO_0000127173"
FT DOMAIN 13..68
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 83..116
FT /note="Orange"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT MOTIF 183..186
FT /note="WRPW motif"
FT VARIANT 155..156
FT /note="Missing (in strain: NVIII-9 and NVIII-46)"
SQ SEQUENCE 186 AA; 20687 MW; 9330530963CDCE15 CRC64;
MATKYEMSKT YQYRKVMKPL LERKRRARIN KCLDELKDLM AECVAQTGDA KFEKADILEV
TVQHLRKLKE SKKHVPANPE QSFRAGYIRA ANEVSRALAS LPRVDVAFGT TLMTHLGMRL
NQLEQPMEQP QAVNTPLSIV CGSSSSSSTY SSASSCSSIS PVSSGYASDN ESLLQISSPG
QVWRPW
