ESM8_DROHY
ID ESM8_DROHY Reviewed; 183 AA.
AC Q07291;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Enhancer of split m8 protein;
DE Short=E(spl)m8;
GN Name=E(spl)m8-HLH {ECO:0000250|UniProtKB:P13098};
GN Synonyms=E(spl) {ECO:0000250|UniProtKB:P13098},
GN M8 {ECO:0000250|UniProtKB:P13098};
OS Drosophila hydei (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila.
OX NCBI_TaxID=7224;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8516287; DOI=10.1073/pnas.90.12.5464;
RA Maier D., Marte B.M., Schaefer W., Yu Y., Preiss A.;
RT "Drosophila evolution challenges postulated redundancy in the E(spl) gene
RT complex.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:5464-5468(1993).
CC -!- FUNCTION: Participates in the control of cell fate choice by
CC uncommitted neuroectodermal cells in the embryo. Transcriptional
CC repressor. Binds DNA on N-box motifs: 5'-CACNAG-3'.
CC -!- SUBUNIT: Transcription repression requires formation of a complex with
CC a corepressor protein (Groucho). Forms homodimers (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DOMAIN: The orange domain and the basic helix-loop-helix motif mediate
CC repression of specific transcriptional activators, such as basic helix-
CC loop-helix protein dimers.
CC -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC domain necessary for the interaction with Groucho, a transcriptional
CC corepressor recruited to specific target DNA by Hairy-related proteins.
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DR EMBL; X71662; CAA50654.1; -; Genomic_DNA.
DR PIR; S36038; S36038.
DR AlphaFoldDB; Q07291; -.
DR SMR; Q07291; -.
DR DIP; DIP-751N; -.
DR ELM; Q07291; -.
DR FlyBase; FBgn0012364; Dhyd\E(spl).
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR003650; Orange_dom.
DR Pfam; PF07527; Hairy_orange; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00511; ORANGE; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS51054; ORANGE; 1.
PE 3: Inferred from homology;
KW Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..183
FT /note="Enhancer of split m8 protein"
FT /id="PRO_0000127174"
FT DOMAIN 10..65
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 86..119
FT /note="Orange"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT REGION 152..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 180..183
FT /note="WRPW motif"
SQ SEQUENCE 183 AA; 20396 MW; 0522DD18BE441B24 CRC64;
MEYTTKTQIY QKVKKPLLER QRRARINKCL DTLKTLVADV RGDDGILRMD KAEMLESAVV
FMRQQKTGKS TEIPAAATPP MPLESFRNGY MNAVNEVSRV MASTPGMSVD LGKSVMSHLG
RVYKNLQQFH EAQTMSESEA ATELSINCNS APLSPASSGY HSDCESPAPS PMPEEGEKLW
RPW
