ESMB_DROME
ID ESMB_DROME Reviewed; 195 AA.
AC Q01069; Q01906; Q540X9; Q5S4K3; Q9VBJ0;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=Enhancer of split mbeta protein;
DE Short=E(spl)mbeta;
DE AltName: Full=HLH-mbeta;
DE AltName: Full=Split locus enhancer protein mA;
GN Name=E(spl)mbeta-HLH {ECO:0000312|FlyBase:FBgn0002733}; Synonyms=HLHmbeta;
GN ORFNames=CG14548 {ECO:0000312|FlyBase:FBgn0002733};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=Canton-S; TISSUE=Embryo;
RX PubMed=1528887; DOI=10.1073/pnas.89.18.8731;
RA Delidakis C., Artavanis-Tsakonas S.;
RT "The Enhancer of split [E(spl)] locus of Drosophila encodes seven
RT independent helix-loop-helix proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:8731-8735(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE, AND
RP VARIANT AT GLY-170.
RC STRAIN=Oregon-R;
RX PubMed=1427040; DOI=10.1093/genetics/132.2.505;
RA Knust E., Schrons H., Grawe F., Campos-Ortega J.A.;
RT "Seven genes of the Enhancer of split complex of Drosophila melanogaster
RT encode helix-loop-helix proteins.";
RL Genetics 132:505-518(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT AT GLY-170.
RC STRAIN=NVIII-1, NVIII-18, NVIII-2, NVIII-22, NVIII-24, NVIII-28, NVIII-41,
RC NVIII-42, NVIII-46, NVIII-5, NVIII-9, NVIII-m11, NVIII-m12, NVIII-m13,
RC NVIII-m15, and NVIII-m19;
RX PubMed=15537803; DOI=10.1093/molbev/msi046;
RA Macdonald S.J., Long A.D.;
RT "Identifying signatures of selection at the enhancer of split neurogenic
RT gene complex in Drosophila.";
RL Mol. Biol. Evol. 22:607-619(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [7]
RP DEVELOPMENTAL STAGE.
RX PubMed=22357926; DOI=10.1242/dev.071779;
RA Zacharioudaki E., Magadi S.S., Delidakis C.;
RT "bHLH-O proteins are crucial for Drosophila neuroblast self-renewal and
RT mediate Notch-induced overproliferation.";
RL Development 139:1258-1269(2012).
CC -!- FUNCTION: Transcriptional repressor of genes that require a bHLH
CC protein for their transcription (By similarity). May serve as a
CC transcriptional regulator of the Achaete-scute complex (AS-C) genes
CC (PubMed:1528887). Contributes to the neural-epidermal lineage decision
CC during early neurogenesis (PubMed:1427040). Part of the Notch signaling
CC pathway (PubMed:1427040). {ECO:0000250|UniProtKB:P14003,
CC ECO:0000269|PubMed:1427040, ECO:0000269|PubMed:1528887}.
CC -!- SUBUNIT: Transcription repression requires formation of a complex with
CC a corepressor protein (Groucho). {ECO:0000250|UniProtKB:P14003,
CC ECO:0000250|UniProtKB:Q26263}.
CC -!- INTERACTION:
CC Q01069; O16867: tap; NbExp=3; IntAct=EBI-94554, EBI-102737;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: In larvae, detected in neuroblasts (at protein
CC level) (PubMed:22357926). In imaginal disks, expressed as follows: in
CC the eye disk, within and posterior to the morphogenetic furrow; in the
CC wing pouch, in the presumptive intervein regions and wing margin; in
CC the leg disk, general expression (PubMed:1427040).
CC {ECO:0000269|PubMed:1427040, ECO:0000269|PubMed:22357926}.
CC -!- DOMAIN: Has a particular type of basic domain (presence of a helix-
CC interrupting proline) that binds to the N-box (CACNAG), rather than the
CC canonical E-box (CANNTG).
CC -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC domain necessary for the interaction with Groucho, a transcriptional
CC corepressor recruited to specific target DNA by Hairy-related proteins.
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DR EMBL; M96166; AAA28909.1; -; mRNA.
DR EMBL; X67047; CAA47432.1; -; Genomic_DNA.
DR EMBL; AY779906; AAV59047.1; -; Genomic_DNA.
DR EMBL; AY779907; AAV59059.1; -; Genomic_DNA.
DR EMBL; AY779908; AAV59071.1; -; Genomic_DNA.
DR EMBL; AY779909; AAV59083.1; -; Genomic_DNA.
DR EMBL; AY779910; AAV59095.1; -; Genomic_DNA.
DR EMBL; AY779911; AAV59107.1; -; Genomic_DNA.
DR EMBL; AY779912; AAV59119.1; -; Genomic_DNA.
DR EMBL; AY779913; AAV59131.1; -; Genomic_DNA.
DR EMBL; AY779914; AAV59143.1; -; Genomic_DNA.
DR EMBL; AY779915; AAV59155.1; -; Genomic_DNA.
DR EMBL; AY779916; AAV59167.1; -; Genomic_DNA.
DR EMBL; AY779917; AAV59179.1; -; Genomic_DNA.
DR EMBL; AY779918; AAV59191.1; -; Genomic_DNA.
DR EMBL; AY779919; AAV59203.1; -; Genomic_DNA.
DR EMBL; AY779920; AAV59215.1; -; Genomic_DNA.
DR EMBL; AY779921; AAV59227.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF56546.1; -; Genomic_DNA.
DR EMBL; AY118723; AAM50583.1; -; mRNA.
DR PIR; C46177; C46177.
DR RefSeq; NP_524505.2; NM_079781.3.
DR AlphaFoldDB; Q01069; -.
DR SMR; Q01069; -.
DR BioGRID; 68052; 24.
DR DIP; DIP-721N; -.
DR ELM; Q01069; -.
DR IntAct; Q01069; 16.
DR STRING; 7227.FBpp0084355; -.
DR PaxDb; Q01069; -.
DR DNASU; 43152; -.
DR EnsemblMetazoa; FBtr0084982; FBpp0084355; FBgn0002733.
DR GeneID; 43152; -.
DR KEGG; dme:Dmel_CG14548; -.
DR CTD; 43152; -.
DR FlyBase; FBgn0002733; E(spl)mbeta-HLH.
DR VEuPathDB; VectorBase:FBgn0002733; -.
DR eggNOG; KOG4304; Eukaryota.
DR GeneTree; ENSGT00940000167178; -.
DR HOGENOM; CLU_068550_2_2_1; -.
DR InParanoid; Q01069; -.
DR OMA; THCANEV; -.
DR OrthoDB; 1483774at2759; -.
DR PhylomeDB; Q01069; -.
DR SignaLink; Q01069; -.
DR BioGRID-ORCS; 43152; 0 hits in 3 CRISPR screens.
DR ChiTaRS; E(spl)mbeta-HLH; fly.
DR GenomeRNAi; 43152; -.
DR PRO; PR:Q01069; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0002733; Expressed in wing disc and 72 other tissues.
DR Genevisible; Q01069; DM.
DR GO; GO:0005634; C:nucleus; IMP:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:UniProtKB.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0002052; P:positive regulation of neuroblast proliferation; IMP:UniProtKB.
DR GO; GO:1902692; P:regulation of neuroblast proliferation; IMP:UniProtKB.
DR GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR003650; Orange_dom.
DR Pfam; PF07527; Hairy_orange; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00511; ORANGE; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS51054; ORANGE; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Neurogenesis;
KW Notch signaling pathway; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..195
FT /note="Enhancer of split mbeta protein"
FT /id="PRO_0000127176"
FT DOMAIN 13..70
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 99..132
FT /note="Orange"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT REGION 163..195
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 192..195
FT /note="WRPW motif"
FT VARIANT 170
FT /note="D -> G (in strain: Oregon-R, NVIII-1, NVIII-18,
FT NVIII-2, NVIII-22, NVIII-24, NVIII-28, NVIII-41, NVIII-42,
FT NVIII-46, NVIII-5, NVIII-9, NVIII-m11, NVIII-m12, NVIII-
FT m13, NVIII-m15 and NVIII-m19)"
FT /evidence="ECO:0000269|PubMed:1427040,
FT ECO:0000269|PubMed:15537803"
FT CONFLICT 171
FT /note="S -> T (in Ref. 1; AAA28909)"
FT /evidence="ECO:0000305"
FT CONFLICT 176
FT /note="A -> R (in Ref. 1; AAA28909)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 195 AA; 21494 MW; 8ECAAC7E55D081DC CRC64;
MVLEMEMSKT YQYRKVMKPM LERKRRARIN KCLDELKDIM VECLTQEGEH ITRLEKADIL
ELTVEHMKKL RAQKQLRLSS VTGGVSPSAD PKLSIAESFR AGYVHAANEV SKTLAAVPGV
SVDLGTQLMS HLGHRLNYLQ VVVPSLPIGV PLQAPVEDQA MVTPPPSECD SLESGACSPA
PSEASSTSGP MWRPW
