ESMC_DROME
ID ESMC_DROME Reviewed; 205 AA.
AC Q01070; Q01898; Q5S4K4;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 182.
DE RecName: Full=Enhancer of split mgamma protein;
DE Short=E(spl)mgamma;
DE AltName: Full=Split locus enhancer protein mB;
GN Name=E(spl)mgamma-HLH {ECO:0000312|FlyBase:FBgn0002735};
GN Synonyms=HLHmgamma {ECO:0000312|FlyBase:FBgn0002735};
GN ORFNames=CG8333 {ECO:0000312|FlyBase:FBgn0002735};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=Oregon-R; TISSUE=Embryo;
RX PubMed=1427040; DOI=10.1093/genetics/132.2.505;
RA Knust E., Schrons H., Grawe F., Campos-Ortega J.A.;
RT "Seven genes of the Enhancer of split complex of Drosophila melanogaster
RT encode helix-loop-helix proteins.";
RL Genetics 132:505-518(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC TISSUE=Embryo;
RX PubMed=1528887; DOI=10.1073/pnas.89.18.8731;
RA Delidakis C., Artavanis-Tsakonas S.;
RT "The Enhancer of split [E(spl)] locus of Drosophila encodes seven
RT independent helix-loop-helix proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:8731-8735(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=10790334; DOI=10.1006/dbio.2000.9691;
RA Cooper M.T.D., Tyler D.M., Furriols M., Chalkiadaki A., Delidakis C.,
RA Bray S.;
RT "Spatially restricted factors cooperate with Notch in the regulation of
RT Enhancer of split genes.";
RL Dev. Biol. 221:390-403(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NVIII-1, NVIII-18, NVIII-2, NVIII-22, NVIII-24, NVIII-28, NVIII-41,
RC NVIII-42, NVIII-46, NVIII-5, NVIII-9, NVIII-m11, NVIII-m12, NVIII-m13,
RC NVIII-m15, and NVIII-m19;
RX PubMed=15537803; DOI=10.1093/molbev/msi046;
RA Macdonald S.J., Long A.D.;
RT "Identifying signatures of selection at the enhancer of split neurogenic
RT gene complex in Drosophila.";
RL Mol. Biol. Evol. 22:607-619(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP FUNCTION, SUBUNIT, INTERACTION WITH DPN, AND DEVELOPMENTAL STAGE.
RX PubMed=22357926; DOI=10.1242/dev.071779;
RA Zacharioudaki E., Magadi S.S., Delidakis C.;
RT "bHLH-O proteins are crucial for Drosophila neuroblast self-renewal and
RT mediate Notch-induced overproliferation.";
RL Development 139:1258-1269(2012).
RN [9]
RP FUNCTION.
RX PubMed=24618901; DOI=10.7554/elife.01906;
RA Koe C.T., Li S., Rossi F., Wong J.J., Wang Y., Zhang Z., Chen K., Aw S.S.,
RA Richardson H.E., Robson P., Sung W.K., Yu F., Gonzalez C., Wang H.;
RT "The Brm-HDAC3-Erm repressor complex suppresses dedifferentiation in
RT Drosophila type II neuroblast lineages.";
RL Elife 3:E01906-E01906(2014).
CC -!- FUNCTION: Transcriptional repressor of genes that require a bHLH
CC protein for their transcription (PubMed:24618901). May serve as a
CC transcriptional regulator of the Achaete-scute complex (AS-C) genes
CC (PubMed:1528887). Contributes to the neural-epidermal lineage decision
CC during early neurogenesis (PubMed:1427040). Part of the Notch signaling
CC pathway, plays a role in neuroblasts proliferation in embryos and
CC larvae (PubMed:10790334, PubMed:22357926). In the larval brain,
CC together with other self-renewal transcriptional repressors such as klu
CC and dpn, required for type II neuroblast self-renewal and for
CC maintaining erm in an inactive state in intermediate neural progenitors
CC (INP) derived from type II neuroblasts (PubMed:24618901).
CC {ECO:0000269|PubMed:10790334, ECO:0000269|PubMed:1427040,
CC ECO:0000269|PubMed:1528887, ECO:0000269|PubMed:22357926,
CC ECO:0000269|PubMed:24618901}.
CC -!- SUBUNIT: Homodimer (PubMed:22357926). Heterodimer with dpn
CC (PubMed:22357926). Might form higher-order oligomers (PubMed:22357926).
CC Transcription repression requires formation of a complex with a
CC corepressor protein (Groucho) (By similarity).
CC {ECO:0000250|UniProtKB:P14003, ECO:0000250|UniProtKB:Q26263,
CC ECO:0000269|PubMed:22357926}.
CC -!- INTERACTION:
CC Q01070; P10083: ac; NbExp=3; IntAct=EBI-119159, EBI-152748;
CC Q01070; Q7JZ51: insb; NbExp=4; IntAct=EBI-119159, EBI-168547;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in sensory organ precursors in the wing,
CC leg and eye imaginal disk. {ECO:0000269|PubMed:10790334}.
CC -!- DEVELOPMENTAL STAGE: In embryo, detected in the neuroectoderm at stage
CC 8, and later in neuroblasts at stage 9 (at protein level)
CC (PubMed:22357926). By stage 16, detected only in the neuroblasts (at
CC protein level) (PubMed:22357926). In larvae, detected in a subset of
CC neuroblasts, some of which will give rise to the optic lobe (at protein
CC level) (PubMed:22357926). In embryo, detected during the initial stages
CC of germ band exclusively within the neuroectoderm, both in the
CC territory of the trunk and in cephalic regions (PubMed:1427040). During
CC stage 9, detected within the procephalic lobe and the ventral ectoderm
CC (PubMed:1427040). {ECO:0000269|PubMed:1427040,
CC ECO:0000269|PubMed:22357926}.
CC -!- DOMAIN: Has a particular type of basic domain (presence of a helix-
CC interrupting proline) that binds to the N-box (CACNAG), rather than the
CC canonical E-box (CANNTG).
CC -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC domain necessary for the interaction with Groucho, a transcriptional
CC corepressor recruited to specific target DNA by Hairy-related proteins.
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DR EMBL; X67049; CAA47434.1; -; mRNA.
DR EMBL; M96167; AAA28910.1; -; Genomic_DNA.
DR EMBL; AJ276315; CAB77021.1; -; Genomic_DNA.
DR EMBL; AY779906; AAV59046.1; -; Genomic_DNA.
DR EMBL; AY779907; AAV59058.1; -; Genomic_DNA.
DR EMBL; AY779908; AAV59070.1; -; Genomic_DNA.
DR EMBL; AY779909; AAV59082.1; -; Genomic_DNA.
DR EMBL; AY779910; AAV59094.1; -; Genomic_DNA.
DR EMBL; AY779911; AAV59106.1; -; Genomic_DNA.
DR EMBL; AY779912; AAV59118.1; -; Genomic_DNA.
DR EMBL; AY779913; AAV59130.1; -; Genomic_DNA.
DR EMBL; AY779914; AAV59142.1; -; Genomic_DNA.
DR EMBL; AY779915; AAV59154.1; -; Genomic_DNA.
DR EMBL; AY779916; AAV59166.1; -; Genomic_DNA.
DR EMBL; AY779917; AAV59178.1; -; Genomic_DNA.
DR EMBL; AY779918; AAV59190.1; -; Genomic_DNA.
DR EMBL; AY779919; AAV59202.1; -; Genomic_DNA.
DR EMBL; AY779920; AAV59214.1; -; Genomic_DNA.
DR EMBL; AY779921; AAV59226.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF56545.1; -; Genomic_DNA.
DR EMBL; BT022125; AAY51520.1; -; mRNA.
DR PIR; B46177; B46177.
DR RefSeq; NP_524504.2; NM_079780.3.
DR AlphaFoldDB; Q01070; -.
DR SMR; Q01070; -.
DR BioGRID; 68051; 27.
DR DIP; DIP-723N; -.
DR ELM; Q01070; -.
DR IntAct; Q01070; 21.
DR STRING; 7227.FBpp0084329; -.
DR PaxDb; Q01070; -.
DR DNASU; 43151; -.
DR EnsemblMetazoa; FBtr0084955; FBpp0084329; FBgn0002735.
DR GeneID; 43151; -.
DR KEGG; dme:Dmel_CG8333; -.
DR CTD; 43151; -.
DR FlyBase; FBgn0002735; E(spl)mgamma-HLH.
DR VEuPathDB; VectorBase:FBgn0002735; -.
DR eggNOG; KOG4304; Eukaryota.
DR GeneTree; ENSGT00940000167178; -.
DR HOGENOM; CLU_068550_2_2_1; -.
DR InParanoid; Q01070; -.
DR OMA; MIHENQI; -.
DR OrthoDB; 1483774at2759; -.
DR PhylomeDB; Q01070; -.
DR SignaLink; Q01070; -.
DR BioGRID-ORCS; 43151; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 43151; -.
DR PRO; PR:Q01070; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0002735; Expressed in eye disc (Drosophila) and 36 other tissues.
DR Genevisible; Q01070; DM.
DR GO; GO:0005634; C:nucleus; IMP:UniProtKB.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:UniProtKB.
DR GO; GO:0008134; F:transcription factor binding; IGI:FlyBase.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0097150; P:neuronal stem cell population maintenance; IMP:FlyBase.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0002052; P:positive regulation of neuroblast proliferation; IMP:UniProtKB.
DR GO; GO:1902692; P:regulation of neuroblast proliferation; IMP:UniProtKB.
DR GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048190; P:wing disc dorsal/ventral pattern formation; IGI:FlyBase.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR003650; Orange_dom.
DR Pfam; PF07527; Hairy_orange; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SMART; SM00511; ORANGE; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
DR PROSITE; PS51054; ORANGE; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Neurogenesis;
KW Notch signaling pathway; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..205
FT /note="Enhancer of split mgamma protein"
FT /id="PRO_0000127177"
FT DOMAIN 15..72
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT DOMAIN 93..126
FT /note="Orange"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT MOTIF 202..205
FT /note="WRPW motif"
FT CONFLICT 166
FT /note="S -> T (in Ref. 2; CAA47434)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 205 AA; 23135 MW; 4183A35D66DAB7E5 CRC64;
MSSLQMSEMS KTYQYRKVMK PMLERKRRAR INKCLDELKD LMVATLESEG EHVTRLEKAD
ILELTVTHLQ KMKQQRQHKR ASGDESLTPA EGFRSGYIHA VNEVSRSLSQ LPGMNVSLGT
QLMTHLGQRL NQIQPAEKEV LPVTAPLSVH IANRDAYSVP ISPISSYAGS PNSNTSSTSH
SLLTTIDVTK MEDDSEDEEN VWRPW
