ID   ESPB_MYCSE              Reviewed;         526 AA.
AC   L8FKX3; A0A2U9PH63;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=ESX-1 secretion-associated protein EspB {ECO:0000305};
DE   AltName: Full=Antigen MTB48 {ECO:0000305};
GN   Name=espB {ECO:0000305};
GN   ORFNames=0076R {ECO:0000303|PubMed:18554329},
GN   D806_000800 {ECO:0000312|EMBL:AWT51074.1}, D806_0079;
OS   Mycolicibacterium smegmatis (strain MKD8) (Mycobacterium smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=1214915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RX   PubMed=23618714; DOI=10.1128/genomea.00148-13;
RA   Gray T.A., Palumbo M.J., Derbyshire K.M.;
RT   "Draft genome sequence of MKD8, a conjugal recipient Mycobacterium
RT   smegmatis strain.";
RL   Genome Announc. 1:E0014813-E0014813(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RA   Derbyshire K., Gray T.A., Champion M.;
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=MKD8;
RX   PubMed=18554329; DOI=10.1111/j.1365-2958.2008.06299.x;
RA   Coros A., Callahan B., Battaglioli E., Derbyshire K.M.;
RT   "The specialized secretory apparatus ESX-1 is essential for DNA transfer in
RT   Mycobacterium smegmatis.";
RL   Mol. Microbiol. 69:794-808(2008).
CC   -!- FUNCTION: Involved in DNA conjugation, at least in the recipient strain
CC       (PubMed:18554329). {ECO:0000269|PubMed:18554329}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P9WJD9}.
CC       Note=Secreted via the ESX-1 / type VII secretion system (T7SS) (By
CC       similarity). {ECO:0000250|UniProtKB:P9WJD9}.
CC   -!- DISRUPTION PHENOTYPE: Loss of DNA conjugation when disrupted in
CC       recipient strain; strain still secretes EsxB (PubMed:18554329).
CC       {ECO:0000269|PubMed:18554329}.
CC   -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC       distinct donor and recipient strains; mc(2)155 is a donor strain while
CC       MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC       transfer do not always alter DNA transfer in a recipient strain.
CC       {ECO:0000305|PubMed:18554329}.
CC   -!- SIMILARITY: Belongs to the EspB family. {ECO:0000305}.
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DR   EMBL; CP027541; AWT51074.1; -; Genomic_DNA.
DR   RefSeq; WP_003891407.1; NZ_CP027541.1.
DR   AlphaFoldDB; L8FKX3; -.
DR   SMR; L8FKX3; -.
DR   EnsemblBacteria; AWT51074; AWT51074; D806_000800.
DR   PATRIC; fig|1214915.3.peg.84; -.
DR   HOGENOM; CLU_506989_0_0_11; -.
DR   Proteomes; UP000011200; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR041275; EspB_PE.
DR   Pfam; PF18625; EspB_PE; 1.
PE   3: Inferred from homology;
KW   Secreted.
FT   CHAIN           1..526
FT                   /note="ESX-1 secretion-associated protein EspB"
FT                   /id="PRO_0000438360"
FT   REGION          1..35
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          91..110
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          271..526
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..30
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        293..307
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        338..377
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        483..526
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   526 AA;  52723 MW;  271DD1B82ECF68AA CRC64;
     MSEELKYELP GLERKAHECE STRPEGPGDA TKPDELATTA SVYGKLMASA AKLKATFAAG
     DREGKRIAAA IRAAAGAYQK IEEQKAAELN RQMNGSDAPP PAAEAVVPDM SGIPGPLTIP
     SMEYPSAAAA ADEMDWEAAA RIIHSGDTQA LSMKYFRDQW RDYQSTLEGH GRHFANPAEG
     WSGAAAETCA EAQRRLSTWW ADMGAECGRL AQEATTFVDA HDKLVANHPT MDDVKAFEEA
     EWESEWDRQY AWAIKQEKSE DALEAYANGS QIQEIRPGKP PSIGGLPAVN DGDVQATPTS
     TTGGPGGPGS GSGGGSGGGA SGGSGGGTPE MPELPSTDPS MSPMSTNSAG EEQSSGSPSS
     GGSSSGGSPS GGSPSGGGAP SSGGMPEGGL PTDMPGGPDI PGLDDPSLKP ASAGGGGGGG
     VGGGGGGGMP AAPLGPAVGA DSVSPSPSST RGGGVGVPTG TGGGAGGMMG GGMGGMGAGH
     GQGQGKEKKR DPKLAPDEDL YTEDRAHSEG VIGHRPRREK DSGKQQ