ESPD_MYCTU
ID ESPD_MYCTU Reviewed; 184 AA.
AC P9WJD5; L0TEQ0; O06269; Q7D573;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 39.
DE RecName: Full=ESX-1 secretion-associated protein EspD {ECO:0000305};
GN Name=espD; Synonyms=snm10 {ECO:0000303|PubMed:16135231};
GN OrderedLocusNames=Rv3614c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP FUNCTION, AND INTERACTION WITH ECCE1.
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=16135231; DOI=10.1111/j.1365-2958.2005.04800.x;
RA MacGurn J.A., Raghavan S., Stanley S.A., Cox J.S.;
RT "A non-RD1 gene cluster is required for Snm secretion in Mycobacterium
RT tuberculosis.";
RL Mol. Microbiol. 57:1653-1663(2005).
RN [3]
RP REGULATION BY PHOP/PHOR.
RC STRAIN=H37Rv;
RX PubMed=16573683; DOI=10.1111/j.1365-2958.2006.05102.x;
RA Walters S.B., Dubnau E., Kolesnikova I., Laval F., Daffe M., Smith I.;
RT "The Mycobacterium tuberculosis PhoPR two-component system regulates genes
RT essential for virulence and complex lipid biosynthesis.";
RL Mol. Microbiol. 60:312-330(2006).
RN [4]
RP REGULATION BY ESPR.
RX PubMed=18685700; DOI=10.1038/nature07219;
RA Raghavan S., Manzanillo P., Chan K., Dovey C., Cox J.S.;
RT "Secreted transcription factor controls Mycobacterium tuberculosis
RT virulence.";
RL Nature 454:717-721(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP TRP-19; TRP-27; PRO-31; ARG-138 AND TRP-150.
RC STRAIN=ATCC 35801 / TMC 107 / Erdman, and H37Rv;
RX PubMed=22155774; DOI=10.1128/jb.06417-11;
RA Chen J.M., Boy-Roettger S., Dhar N., Sweeney N., Buxton R.S., Pojer F.,
RA Rosenkrands I., Cole S.T.;
RT "EspD is critical for the virulence-mediating ESX-1 secretion system in
RT Mycobacterium tuberculosis.";
RL J. Bacteriol. 194:884-893(2012).
RN [7]
RP REGULATION BY ESPR.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=22389481; DOI=10.1128/jb.00142-12;
RA Hunt D.M., Sweeney N.P., Mori L., Whalan R.H., Comas I., Norman L.,
RA Cortes T., Arnvig K.B., Davis E.O., Stapleton M.R., Green J., Buxton R.S.;
RT "Long-range transcriptional control of an operon necessary for virulence-
RT critical ESX-1 secretion in Mycobacterium tuberculosis.";
RL J. Bacteriol. 194:2307-2320(2012).
RN [8]
RP REGULATION BY MPRA/MPRB.
RC STRAIN=H37Rv;
RX PubMed=23104803; DOI=10.1128/jb.01067-12;
RA Pang X., Samten B., Cao G., Wang X., Tvinnereim A.R., Chen X.L.,
RA Howard S.T.;
RT "MprAB regulates the espA operon in Mycobacterium tuberculosis and
RT modulates ESX-1 function and host cytokine response.";
RL J. Bacteriol. 195:66-75(2013).
RN [9]
RP TRANSCRIPTIONAL REGULATION.
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=25536998; DOI=10.1099/mic.0.000023;
RA Cao G., Howard S.T., Zhang P., Wang X., Chen X.L., Samten B., Pang X.;
RT "EspR, a regulator of the ESX-1 secretion system in Mycobacterium
RT tuberculosis, is directly regulated by the two-component systems MprAB and
RT PhoPR.";
RL Microbiology 161:477-489(2015).
CC -!- FUNCTION: Required for ESX-1 function (PubMed:16135231,
CC PubMed:22155774). Required for the maintenance of adequate cellular
CC levels of both EspA and EspC (PubMed:22155774). Facilitates EsxA
CC secretion (PubMed:22155774). {ECO:0000269|PubMed:16135231,
CC ECO:0000269|PubMed:22155774}.
CC -!- SUBUNIT: Interacts with EccE1. {ECO:0000269|PubMed:16135231}.
CC -!- INTERACTION:
CC P9WJD5; P9WJB9: espL; NbExp=2; IntAct=EBI-26358082, EBI-25767670;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22155774}.
CC Note=Export does not exclusively require ESX-1. May require one (or
CC more) of the other ESX / type VII secretion systems (T7SS).
CC {ECO:0000269|PubMed:22155774}.
CC -!- INDUCTION: Transcriptionally activated by EspR (PubMed:18685700,
CC PubMed:22389481, PubMed:25536998). Repressed by the MprB/MprA two-
CC component system, by direct regulation and via EspR (PubMed:23104803,
CC PubMed:25536998). Up-regulated by the PhoP/PhoR two-component system,
CC via EspR (PubMed:16573683, PubMed:25536998).
CC {ECO:0000269|PubMed:16573683, ECO:0000269|PubMed:18685700,
CC ECO:0000269|PubMed:22389481, ECO:0000269|PubMed:23104803,
CC ECO:0000269|PubMed:25536998}.
CC -!- DISRUPTION PHENOTYPE: EspD deficiency results in decreased cellular
CC levels of EspA and EspC, but does not affect their transcription.
CC {ECO:0000269|PubMed:22155774}.
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DR EMBL; AL123456; CCP46437.1; -; Genomic_DNA.
DR PIR; G70956; G70956.
DR RefSeq; NP_218131.1; NC_000962.3.
DR RefSeq; WP_003419545.1; NZ_NVQJ01000056.1.
DR AlphaFoldDB; P9WJD5; -.
DR SMR; P9WJD5; -.
DR BioGRID; 4355263; 1.
DR IntAct; P9WJD5; 1.
DR MINT; P9WJD5; -.
DR STRING; 83332.Rv3614c; -.
DR PaxDb; P9WJD5; -.
DR DNASU; 885777; -.
DR GeneID; 45427600; -.
DR GeneID; 885777; -.
DR KEGG; mtu:Rv3614c; -.
DR PATRIC; fig|83332.111.peg.4020; -.
DR TubercuList; Rv3614c; -.
DR eggNOG; ENOG5031JYJ; Bacteria.
DR OMA; DSASEQC; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0044315; P:protein secretion by the type VII secretion system; IMP:MTBBASE.
PE 1: Evidence at protein level;
KW Reference proteome; Secreted; Virulence.
FT CHAIN 1..184
FT /note="ESX-1 secretion-associated protein EspD"
FT /id="PRO_0000393904"
FT REGION 33..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 19
FT /note="W->R: Absence of EspD secretion."
FT /evidence="ECO:0000269|PubMed:22155774"
FT MUTAGEN 27
FT /note="W->R: Absence of EspD secretion."
FT /evidence="ECO:0000269|PubMed:22155774"
FT MUTAGEN 31
FT /note="P->A: Decreased EspD secretion and small reduction
FT in cellular EspA levels."
FT /evidence="ECO:0000269|PubMed:22155774"
FT MUTAGEN 138
FT /note="R->A: Absence of EsxA secretion. Decreases EspD
FT stability."
FT /evidence="ECO:0000269|PubMed:22155774"
FT MUTAGEN 150
FT /note="W->R: Absence of EspD secretion. Decreases EspA
FT levels."
FT /evidence="ECO:0000269|PubMed:22155774"
SQ SEQUENCE 184 AA; 19835 MW; 6326911F65DC5C7A CRC64;
MDLPGNDFDS NDFDAVDLWG ADGAEGWTAD PIIGVGSAAT PDTGPDLDNA HGQAETDTEQ
EIALFTVTNP PRTVSVSTLM DGRIDHVELS ARVAWMSESQ LASEILVIAD LARQKAQSAQ
YAFILDRMSQ QVDADEHRVA LLRKTVGETW GLPSPEEAAA AEAEVFATRY SDDCPAPDDE
SDPW
