ID   ESPE_MYCS2              Reviewed;         525 AA.
AC   A0QNI5; I7FCC6;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=ESX-1 secretion-associated protein EspE {ECO:0000303|PubMed:22233444};
GN   Name=espE {ECO:0000303|PubMed:22233444};
GN   OrderedLocusNames=MSMEG_0055, MSMEI_0056;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=22233444; DOI=10.1111/j.1365-2958.2011.07958.x;
RA   Wirth S.E., Krywy J.A., Aldridge B.B., Fortune S.M., Fernandez-Suarez M.,
RA   Gray T.A., Derbyshire K.M.;
RT   "Polar assembly and scaffolding proteins of the virulence-associated ESX-1
RT   secretory apparatus in mycobacteria.";
RL   Mol. Microbiol. 83:654-664(2012).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22233444}.
CC       Note=Localizes at or near the cell pole in (on average) 1 discrete spot
CC       that colocalizes with SaeC (PubMed:22233444).
CC       {ECO:0000269|PubMed:22233444}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AFP36538.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP000480; ABK71412.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP36538.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_011726636.1; NZ_SIJM01000058.1.
DR   RefSeq; YP_884473.1; NC_008596.1.
DR   AlphaFoldDB; A0QNI5; -.
DR   STRING; 246196.MSMEI_0056; -.
DR   EnsemblBacteria; ABK71412; ABK71412; MSMEG_0055.
DR   EnsemblBacteria; AFP36538; AFP36538; MSMEI_0056.
DR   GeneID; 66738245; -.
DR   KEGG; msg:MSMEI_0056; -.
DR   KEGG; msm:MSMEG_0055; -.
DR   PATRIC; fig|246196.19.peg.53; -.
DR   eggNOG; ENOG503200Z; Bacteria.
DR   OMA; WTINIVE; -.
DR   OrthoDB; 1790169at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR043796; ESX-1_EspA/EspE-like.
DR   Pfam; PF18879; EspA_EspE; 1.
PE   4: Predicted;
KW   Cytoplasm; Reference proteome.
FT   CHAIN           1..525
FT                   /note="ESX-1 secretion-associated protein EspE"
FT                   /id="PRO_0000438361"
FT   REGION          244..341
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          375..494
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        270..284
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        415..454
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   525 AA;  53068 MW;  14209F309EF34943 CRC64;
     MGVLSDVADF GKDVIDDRDK WADRFKKVGH FIERNAKGDR LERVAKFGRA LGDFSGKFTD
     FFESNLGKRM VKAARSPILA AGQHVITGMK LTTGVGDPEN GRRFGEGADH MSAAGRTLDS
     AYPTSDWDSA ASDAYLSQNN GQVTRAEALV HADQVVAAVL SREAEQIATT REVLDSEADW
     LGDMSLVTMA TGLIPYVGRA AQTAAEIAMV TKAVGASTDQ FMMMRDKADE NAAEVRDAIG
     KYEAVAGDAD DTTADDTAGD APESEPTAAE DSSETSKEDG QSRHENPVAA PSGGGGGATS
     GGGGGAPSSA SSAGPAGTPQ VPSPPGFGAA NTPTDAQPGA AAASDAAGML GSVMGAMLGP
     LGGIVGGVVQ AAGQALQAAT QAGAQAAQLA GQAAAAPDVD RADTDEDTDK DPDAEGDKDS
     DKRDGEGKED GTAPRDREST DAMGADDDDR NAHPQAGSGT DSAGEDDKKP AMTLPPDLQA
     ASALDTGAGS APVHVGADFE HSQLRTVAAA TLDHGIPGSA AARGA