ID   ESPF_MYCTU              Reviewed;         103 AA.
AC   P9WJD1; L0TE06; P96212; Q7D4P8;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=ESX-1 secretion-associated protein EspF {ECO:0000305};
GN   Name=espF {ECO:0000303|PubMed:19682254}; OrderedLocusNames=Rv3865;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=16368961; DOI=10.1128/iai.74.1.88-98.2006;
RA   Brodin P., Majlessi L., Marsollier L., de Jonge M.I., Bottai D.,
RA   Demangel C., Hinds J., Neyrolles O., Butcher P.D., Leclerc C., Cole S.T.,
RA   Brosch R.;
RT   "Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on
RT   immunogenicity and virulence.";
RL   Infect. Immun. 74:88-98(2006).
RN   [3]
RP   INTERACTION WITH ESXA; ECCA1 AND ESPC.
RX   PubMed=19682254; DOI=10.1111/j.1365-2958.2009.06821.x;
RA   DiGiuseppe Champion P.A., Champion M.M., Manzanillo P., Cox J.S.;
RT   "ESX-1 secreted virulence factors are recognized by multiple cytosolic AAA
RT   ATPases in pathogenic mycobacteria.";
RL   Mol. Microbiol. 73:950-962(2009).
RN   [4]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=21196469; DOI=10.1093/infdis/jiq089;
RA   Bottai D., Majlessi L., Simeone R., Frigui W., Laurent C., Lenormand P.,
RA   Chen J., Rosenkrands I., Huerre M., Leclerc C., Cole S.T., Brosch R.;
RT   "ESAT-6 secretion-independent impact of ESX-1 genes espF and espG1 on
RT   virulence of Mycobacterium tuberculosis.";
RL   J. Infect. Dis. 203:1155-1164(2011).
RN   [5]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, CLEAVAGE OF INITIATOR
RP   METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP   [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- SUBUNIT: Interacts with EsxA, EccA1, and EspC.
CC       {ECO:0000269|PubMed:19682254}.
CC   -!- DISRUPTION PHENOTYPE: Inactivation leads to the attenuation of the
CC       recombinant strain, in spite of strong EsxA (ESAT-6) secretion and
CC       generation of specific T-cell responses. Mutant has lower amounts of
CC       PPE68. {ECO:0000269|PubMed:16368961, ECO:0000269|PubMed:21196469}.
CC   -!- SIMILARITY: Belongs to the EspC family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP46694.1; -; Genomic_DNA.
DR   PIR; F70656; F70656.
DR   RefSeq; NP_218382.1; NC_000962.3.
DR   RefSeq; WP_003899737.1; NZ_NVQJ01000074.1.
DR   AlphaFoldDB; P9WJD1; -.
DR   SMR; P9WJD1; -.
DR   STRING; 83332.Rv3865; -.
DR   iPTMnet; P9WJD1; -.
DR   PaxDb; P9WJD1; -.
DR   DNASU; 886172; -.
DR   GeneID; 45427869; -.
DR   GeneID; 886172; -.
DR   KEGG; mtu:Rv3865; -.
DR   TubercuList; Rv3865; -.
DR   eggNOG; ENOG50316IZ; Bacteria.
DR   OMA; YLNSDQG; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   InterPro; IPR022536; EspC.
DR   Pfam; PF10824; T7SS_ESX_EspC; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0007744|PubMed:21969609"
FT   CHAIN           2..103
FT                   /note="ESX-1 secretion-associated protein EspF"
FT                   /id="PRO_0000393951"
FT   MOD_RES         2
FT                   /note="N-acetylthreonine"
FT                   /evidence="ECO:0007744|PubMed:21969609"
SQ   SEQUENCE   103 AA;  10618 MW;  F26C948CE0F0299A CRC64;
     MTGFLGVVPS FLKVLAGMHN EIVGDIKRAT DTVAGISGRV QLTHGSFTSK FNDTLQEFET
     TRSSTGTGLQ GVTSGLANNL LAAAGAYLKA DDGLAGVIDK IFG