ID   ESPG1_MYCS2             Reviewed;         282 AA.
AC   A0QNI7; I7F4H5;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=ESX-1 secretion-associated protein EspG1;
GN   Name=espG1 {ECO:0000303|PubMed:19876390};
GN   Synonyms=Sm3866 {ECO:0000303|PubMed:15687187},
GN   snm5 {ECO:0000303|PubMed:15687187};
GN   OrderedLocusNames=MSMEG_0057, MSMEI_0058;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=15687187; DOI=10.1128/jb.187.4.1238-1245.2005;
RA   Converse S.E., Cox J.S.;
RT   "A protein secretion pathway critical for Mycobacterium tuberculosis
RT   virulence is conserved and functional in Mycobacterium smegmatis.";
RL   J. Bacteriol. 187:1238-1245(2005).
RN   [5]
RP   NOMENCLATURE.
RX   PubMed=19876390; DOI=10.1371/journal.ppat.1000507;
RA   Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S.,
RA   Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C.,
RA   Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.;
RT   "Systematic genetic nomenclature for type VII secretion systems.";
RL   PLoS Pathog. 5:E1000507-E1000507(2009).
CC   -!- FUNCTION: Part of the ESX-1 / type VII specialized secretion system
CC       (T7SS), which exports several proteins including EsxA and EsxB
CC       (PubMed:15687187). Specific chaperone for cognate PE/PPE proteins,
CC       plays an important role in preventing aggregation of PE/PPE dimers (By
CC       similarity). Also plays a role in DNA conjugation, in at least
CC       recipient strain (By similarity). {ECO:0000250|UniProtKB:B2HMS9,
CC       ECO:0000250|UniProtKB:L8FPI5, ECO:0000269|PubMed:15687187,
CC       ECO:0000305|PubMed:19876390}.
CC   -!- SUBUNIT: Interacts specifically with ESX-1-dependent PE/PPE proteins
CC       (By similarity). {ECO:0000250|UniProtKB:B2HMS9}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B2HSU5}.
CC   -!- DISRUPTION PHENOTYPE: EsxA and EsxB secretion severely reduced, but not
CC       their expression (PubMed:15687187). {ECO:0000269|PubMed:15687187}.
CC   -!- SIMILARITY: Belongs to the EspG family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AFP36540.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP000480; ABK75056.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP36540.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_003891384.1; NZ_SIJM01000058.1.
DR   RefSeq; YP_884475.1; NC_008596.1.
DR   AlphaFoldDB; A0QNI7; -.
DR   SMR; A0QNI7; -.
DR   STRING; 246196.MSMEI_0058; -.
DR   EnsemblBacteria; ABK75056; ABK75056; MSMEG_0057.
DR   EnsemblBacteria; AFP36540; AFP36540; MSMEI_0058.
DR   GeneID; 66738247; -.
DR   KEGG; msg:MSMEI_0058; -.
DR   KEGG; msm:MSMEG_0057; -.
DR   PATRIC; fig|246196.19.peg.55; -.
DR   eggNOG; ENOG502ZNK2; Bacteria.
DR   OMA; TLEARWW; -.
DR   OrthoDB; 1266830at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR025734; EspG.
DR   Pfam; PF14011; ESX-1_EspG; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Reference proteome.
FT   CHAIN           1..282
FT                   /note="ESX-1 secretion-associated protein EspG1"
FT                   /id="PRO_0000438363"
SQ   SEQUENCE   282 AA;  30582 MW;  90752E621BF0887E CRC64;
     MTAPFTAETG DAHIDDVVGV EVTIDGMLVI ADKLGLTDFP PSMGIRLNIP QPDLRKVVWE
     QVERDLSAQG VLDVYGNPHP EVAAMVDTLA RADRTLECRW WRRDAGGKMI RFVVCRKGGR
     HVVAARDNDL LVLQRVAPQI GLAGMVMTVL GEGSPANVEP LTGVADRLAQ CRTAEELTGY
     GIPPTSARAY ASIISEPDGW VEIVANERHP GGTTSQVDVA AGVLDAKQGR IVSIPRRVNG
     ELYGSFLSGT KDNLERALEG LVEFLPSKTW FDKTDADNAY QH