ID   ESPG1_MYCSE             Reviewed;         282 AA.
AC   L8FPI5; A0A2U9PH61;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=ESX-1 secretion-associated protein EspG1;
GN   Name=espG1;
GN   ORFNames=0057 {ECO:0000303|PubMed:18554329},
GN   D806_000540 {ECO:0000312|EMBL:AWT51048.1}, D806_0057;
OS   Mycolicibacterium smegmatis (strain MKD8) (Mycobacterium smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=1214915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RX   PubMed=23618714; DOI=10.1128/genomea.00148-13;
RA   Gray T.A., Palumbo M.J., Derbyshire K.M.;
RT   "Draft genome sequence of MKD8, a conjugal recipient Mycobacterium
RT   smegmatis strain.";
RL   Genome Announc. 1:E0014813-E0014813(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RA   Derbyshire K., Gray T.A., Champion M.;
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=MKD8;
RX   PubMed=18554329; DOI=10.1111/j.1365-2958.2008.06299.x;
RA   Coros A., Callahan B., Battaglioli E., Derbyshire K.M.;
RT   "The specialized secretory apparatus ESX-1 is essential for DNA transfer in
RT   Mycobacterium smegmatis.";
RL   Mol. Microbiol. 69:794-808(2008).
CC   -!- FUNCTION: Part of the ESX-1 / type VII specialized secretion system
CC       (T7SS), which exports several proteins including EsxA and EsxB (By
CC       similarity). Specific chaperone for cognate PE/PPE proteins, plays an
CC       important role in preventing aggregation of PE/PPE dimers (By
CC       similarity). Also plays a role in DNA conjugation, in at least
CC       recipient strain (PubMed:18554329). {ECO:0000250|UniProtKB:B2HMS9,
CC       ECO:0000269|PubMed:18554329}.
CC   -!- SUBUNIT: Interacts specifically with ESX-1-dependent PE/PPE proteins
CC       (By similarity). {ECO:0000250|UniProtKB:B2HMS9}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B2HSU5}.
CC   -!- DISRUPTION PHENOTYPE: Loss of DNA conjugation when disrupted in
CC       recipient strain, strain does not secrete EsxB (PubMed:18554329).
CC       {ECO:0000269|PubMed:18554329}.
CC   -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC       distinct donor and recipient strains; mc(2)155 is a donor strain while
CC       MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC       transfer do not always alter DNA transfer in a recipient strain.
CC       {ECO:0000305|PubMed:18554329}.
CC   -!- SIMILARITY: Belongs to the EspG family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP027541; AWT51048.1; -; Genomic_DNA.
DR   RefSeq; WP_003891384.1; NZ_CP027541.1.
DR   AlphaFoldDB; L8FPI5; -.
DR   SMR; L8FPI5; -.
DR   EnsemblBacteria; AWT51048; AWT51048; D806_000540.
DR   GeneID; 66738247; -.
DR   PATRIC; fig|1214915.3.peg.57; -.
DR   HOGENOM; CLU_073321_1_0_11; -.
DR   Proteomes; UP000011200; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR025734; EspG.
DR   Pfam; PF14011; ESX-1_EspG; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm.
FT   CHAIN           1..282
FT                   /note="ESX-1 secretion-associated protein EspG1"
FT                   /id="PRO_0000438362"
SQ   SEQUENCE   282 AA;  30582 MW;  90752E621BF0887E CRC64;
     MTAPFTAETG DAHIDDVVGV EVTIDGMLVI ADKLGLTDFP PSMGIRLNIP QPDLRKVVWE
     QVERDLSAQG VLDVYGNPHP EVAAMVDTLA RADRTLECRW WRRDAGGKMI RFVVCRKGGR
     HVVAARDNDL LVLQRVAPQI GLAGMVMTVL GEGSPANVEP LTGVADRLAQ CRTAEELTGY
     GIPPTSARAY ASIISEPDGW VEIVANERHP GGTTSQVDVA AGVLDAKQGR IVSIPRRVNG
     ELYGSFLSGT KDNLERALEG LVEFLPSKTW FDKTDADNAY QH