ESPG3_MYCTU
ID ESPG3_MYCTU Reviewed; 295 AA.
AC P9WJC7; L0T4X8; O53694; Q7DA33;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 32.
DE RecName: Full=ESX-3 secretion-associated protein EspG3 {ECO:0000305};
GN Name=espG3 {ECO:0000303|PubMed:19876390}; OrderedLocusNames=Rv0289;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NOMENCLATURE.
RX PubMed=19876390; DOI=10.1371/journal.ppat.1000507;
RA Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S.,
RA Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C.,
RA Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.;
RT "Systematic genetic nomenclature for type VII secretion systems.";
RL PLoS Pathog. 5:E1000507-E1000507(2009).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [4] {ECO:0007744|PDB:4W4I}
RP X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS).
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=25275011; DOI=10.1073/pnas.1409345111;
RA Ekiert D.C., Cox J.S.;
RT "Structure of a PE-PPE-EspG complex from Mycobacterium tuberculosis reveals
RT molecular specificity of ESX protein secretion.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:14758-14763(2014).
CC -!- FUNCTION: Specific chaperone for cognate PE/PPE proteins. Plays an
CC important role in preventing aggregation of PE/PPE dimers.
CC {ECO:0000250|UniProtKB:O53943}.
CC -!- SUBUNIT: Interacts specifically with ESX-3-dependent PE/PPE proteins.
CC {ECO:0000250|UniProtKB:O53943}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B2HSU5}.
CC -!- SIMILARITY: Belongs to the EspG family. {ECO:0000305}.
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DR EMBL; AL123456; CCP43019.1; -; Genomic_DNA.
DR PIR; G70836; G70836.
DR RefSeq; NP_214803.1; NC_000962.3.
DR RefSeq; WP_003401521.1; NZ_NVQJ01000026.1.
DR PDB; 4W4I; X-ray; 2.85 A; A=1-295.
DR PDB; 5XKL; X-ray; 2.39 A; A=1-295.
DR PDBsum; 4W4I; -.
DR PDBsum; 5XKL; -.
DR AlphaFoldDB; P9WJC7; -.
DR SMR; P9WJC7; -.
DR STRING; 83332.Rv0289; -.
DR PaxDb; P9WJC7; -.
DR DNASU; 886602; -.
DR GeneID; 45424263; -.
DR GeneID; 886602; -.
DR KEGG; mtu:Rv0289; -.
DR TubercuList; Rv0289; -.
DR eggNOG; ENOG5030P1E; Bacteria.
DR OMA; DGPWFPD; -.
DR PhylomeDB; P9WJC7; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR InterPro; IPR025734; EspG.
DR Pfam; PF14011; ESX-1_EspG; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Cytoplasm; Reference proteome.
FT CHAIN 1..295
FT /note="ESX-3 secretion-associated protein EspG3"
FT /id="PRO_0000394154"
FT STRAND 7..11
FT /evidence="ECO:0007829|PDB:5XKL"
FT HELIX 12..21
FT /evidence="ECO:0007829|PDB:5XKL"
FT HELIX 29..31
FT /evidence="ECO:0007829|PDB:5XKL"
FT HELIX 40..56
FT /evidence="ECO:0007829|PDB:5XKL"
FT HELIX 68..78
FT /evidence="ECO:0007829|PDB:5XKL"
FT STRAND 81..89
FT /evidence="ECO:0007829|PDB:5XKL"
FT STRAND 101..107
FT /evidence="ECO:0007829|PDB:5XKL"
FT STRAND 120..127
FT /evidence="ECO:0007829|PDB:5XKL"
FT STRAND 130..136
FT /evidence="ECO:0007829|PDB:5XKL"
FT HELIX 141..149
FT /evidence="ECO:0007829|PDB:5XKL"
FT STRAND 163..166
FT /evidence="ECO:0007829|PDB:5XKL"
FT HELIX 167..178
FT /evidence="ECO:0007829|PDB:5XKL"
FT HELIX 183..190
FT /evidence="ECO:0007829|PDB:5XKL"
FT TURN 194..196
FT /evidence="ECO:0007829|PDB:5XKL"
FT HELIX 197..204
FT /evidence="ECO:0007829|PDB:5XKL"
FT STRAND 210..219
FT /evidence="ECO:0007829|PDB:5XKL"
FT STRAND 222..225
FT /evidence="ECO:0007829|PDB:5XKL"
FT STRAND 230..234
FT /evidence="ECO:0007829|PDB:5XKL"
FT STRAND 239..246
FT /evidence="ECO:0007829|PDB:5XKL"
FT STRAND 252..258
FT /evidence="ECO:0007829|PDB:5XKL"
FT HELIX 261..273
FT /evidence="ECO:0007829|PDB:5XKL"
FT STRAND 275..277
FT /evidence="ECO:0007829|PDB:5XKL"
SQ SEQUENCE 295 AA; 31559 MW; B55062E063E944D7 CRC64;
MDATPNAVEL TVDNAWFIAE TIGAGTFPWV LAITMPYSDA AQRGAFVDRQ RDELTRMGLL
SPQGVINPAV ADWIKVVCFP DRWLDLRYVG PASADGACEL LRGIVALRTG TGKTSNKTGN
GVVALRNAQL VTFTAMDIDD PRALVPILGV GLAHRPPARF DEFSLPTRVG ARADERLRSG
VPLGEVVDYL GIPASARPVV ESVFSGPRSY VEIVAGCNRD GRHTTTEVGL SIVDTSAGRV
LVSPSRAFDG EWVSTFSPGT PFAIAVAIQT LTACLPDGQW FPGQRVSRDF STQSS
