AGRF2_HUMAN
ID AGRF2_HUMAN Reviewed; 708 AA.
AC Q8IZF7; Q2PNZ1; Q86SL6; Q8NGU5; Q8TDT5;
DT 15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Adhesion G-protein coupled receptor F2;
DE AltName: Full=G-protein coupled receptor 111;
DE AltName: Full=G-protein coupled receptor PGR20;
GN Name=ADGRF2; Synonyms=GPR111, PGR20;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=12565841; DOI=10.1016/s0006-291x(03)00026-3;
RA Fredriksson R., Gloriam D.E.I., Hoeglund P.J., Lagerstroem M.C.,
RA Schioeth H.B.;
RT "There exist at least 30 human G-protein-coupled receptors with long
RT Ser/Thr-rich N-termini.";
RL Biochem. Biophys. Res. Commun. 301:725-734(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
RA Suwa M., Sato T., Okouchi I., Arita M., Futami K., Matsumoto S.,
RA Tsutsumi S., Aburatani H., Asai K., Akiyama Y.;
RT "Genome-wide discovery and analysis of human seven transmembrane helix
RT receptor genes.";
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND VARIANT VAL-467.
RC TISSUE=Testis;
RA Bonner T.I., Kauffman D., Nagle J.W.;
RT "Complete coding sequence of GPR111.";
RL Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 293-708.
RX PubMed=12044878; DOI=10.1016/s0014-5793(02)02775-8;
RA Takeda S., Kadowaki S., Haga T., Takaesu H., Mitaku S.;
RT "Identification of G protein-coupled receptor genes from the human genome
RT sequence.";
RL FEBS Lett. 520:97-101(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 326-487 (ISOFORM 1).
RX PubMed=12679517; DOI=10.1073/pnas.0230374100;
RA Vassilatis D.K., Hohmann J.G., Zeng H., Li F., Ranchalis J.E.,
RA Mortrud M.T., Brown A., Rodriguez S.S., Weller J.R., Wright A.C.,
RA Bergmann J.E., Gaitanaris G.A.;
RT "The G protein-coupled receptor repertoires of human and mouse.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:4903-4908(2003).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=20149256; DOI=10.1186/1471-2407-10-40;
RA Lum A.M., Wang B.B., Beck-Engeser G.B., Li L., Channa N., Wabl M.;
RT "Orphan receptor GPR110, an oncogene overexpressed in lung and prostate
RT cancer.";
RL BMC Cancer 10:40-40(2010).
RN [8]
RP NOMENCLATURE.
RX PubMed=25713288; DOI=10.1124/pr.114.009647;
RA Hamann J., Aust G., Arac D., Engel F.B., Formstone C., Fredriksson R.,
RA Hall R.A., Harty B.L., Kirchhoff C., Knapp B., Krishnan A., Liebscher I.,
RA Lin H.H., Martinelli D.C., Monk K.R., Peeters M.C., Piao X., Promel S.,
RA Schoneberg T., Schwartz T.W., Singer K., Stacey M., Ushkaryov Y.A.,
RA Vallon M., Wolfrum U., Wright M.W., Xu L., Langenhan T., Schioth H.B.;
RT "International union of basic and clinical pharmacology. XCIV. Adhesion G
RT protein-coupled receptors.";
RL Pharmacol. Rev. 67:338-367(2015).
CC -!- FUNCTION: Orphan receptor.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8IZF7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8IZF7-2; Sequence=VSP_040379, VSP_040380, VSP_040381;
CC -!- TISSUE SPECIFICITY: High expression in kidney. Up-regulated in lung
CC adenocarcinomas and prostate cancers. {ECO:0000269|PubMed:20149256}.
CC -!- MISCELLANEOUS: Most adhesion GPCRs undergo autoproteolysis at the GPS
CC domain. ADGRF2 is not autoproteolyzed at the GPS motif because of the
CC lack of a consensus catalytic triad sequence within GPS domain.
CC {ECO:0000250|UniProtKB:E9Q4J9}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 2 family.
CC Adhesion G-protein coupled receptor (ADGR) subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC05907.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY140953; AAN46667.1; -; mRNA.
DR EMBL; AB065684; BAC05907.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DQ315369; ABC41928.1; -; mRNA.
DR EMBL; AL356421; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AB083617; BAB89330.1; -; Genomic_DNA.
DR EMBL; AY255612; AAO85124.1; -; mRNA.
DR RefSeq; NP_722581.4; NM_153839.6. [Q8IZF7-2]
DR AlphaFoldDB; Q8IZF7; -.
DR SMR; Q8IZF7; -.
DR BioGRID; 128806; 1.
DR IntAct; Q8IZF7; 1.
DR STRING; 9606.ENSP00000296862; -.
DR ChEMBL; CHEMBL4523892; -.
DR GlyGen; Q8IZF7; 7 sites.
DR iPTMnet; Q8IZF7; -.
DR PhosphoSitePlus; Q8IZF7; -.
DR BioMuta; ADGRF2; -.
DR DMDM; 59797952; -.
DR MassIVE; Q8IZF7; -.
DR PaxDb; Q8IZF7; -.
DR PeptideAtlas; Q8IZF7; -.
DR PRIDE; Q8IZF7; -.
DR ProteomicsDB; 71348; -. [Q8IZF7-1]
DR Antibodypedia; 30795; 103 antibodies from 22 providers.
DR DNASU; 222611; -.
DR GeneID; 222611; -.
DR KEGG; hsa:222611; -.
DR UCSC; uc003oyy.3; human. [Q8IZF7-1]
DR CTD; 222611; -.
DR DisGeNET; 222611; -.
DR GeneCards; ADGRF2; -.
DR HGNC; HGNC:18991; ADGRF2.
DR HPA; ENSG00000164393; Tissue enhanced (fallopian tube, skin).
DR neXtProt; NX_Q8IZF7; -.
DR PharmGKB; PA134984088; -.
DR VEuPathDB; HostDB:ENSG00000164393; -.
DR eggNOG; KOG4193; Eukaryota.
DR HOGENOM; CLU_002753_3_6_1; -.
DR InParanoid; Q8IZF7; -.
DR OMA; THHNGCV; -.
DR OrthoDB; 611778at2759; -.
DR PhylomeDB; Q8IZF7; -.
DR TreeFam; TF316380; -.
DR PathwayCommons; Q8IZF7; -.
DR SignaLink; Q8IZF7; -.
DR BioGRID-ORCS; 222611; 3 hits in 202 CRISPR screens.
DR ChiTaRS; ADGRF2; human.
DR GeneWiki; GPR111; -.
DR GenomeRNAi; 222611; -.
DR Pharos; Q8IZF7; Tdark.
DR PRO; PR:Q8IZF7; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q8IZF7; protein.
DR Bgee; ENSG00000164393; Expressed in right uterine tube and 44 other tissues.
DR ExpressionAtlas; Q8IZF7; baseline and differential.
DR Genevisible; Q8IZF7; HS.
DR GO; GO:0016021; C:integral component of membrane; TAS:GDB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; TAS:GDB.
DR Gene3D; 2.60.220.50; -; 1.
DR InterPro; IPR046338; GAIN_dom_sf.
DR InterPro; IPR017981; GPCR_2-like.
DR InterPro; IPR000832; GPCR_2_secretin-like.
DR InterPro; IPR000203; GPS.
DR Pfam; PF00002; 7tm_2; 1.
DR Pfam; PF01825; GPS; 1.
DR PRINTS; PR00249; GPCRSECRETIN.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
DR PROSITE; PS50221; GPS; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..708
FT /note="Adhesion G-protein coupled receptor F2"
FT /id="PRO_0000070334"
FT TOPO_DOM 1..451
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 452..472
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 473..487
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 488..508
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 509..530
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 531..551
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 552..557
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 558..578
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 579..606
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 607..627
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 628..650
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 651..671
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 672..674
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 675..695
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 696..708
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT DOMAIN 391..441
FT /note="GPS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00098"
FT CARBOHYD 21
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 220
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 252
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 260
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 305
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 313
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 358
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..68
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_040379"
FT VAR_SEQ 69..92
FT /note="PSPNAVSSDQVHCSAGCTHRKCGW -> MTHILLLYYLVFLLPTESCRTLYQ
FT (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_040380"
FT VAR_SEQ 690..708
FT /note="VSPDASDQVQSERIHEDVL -> GFFILVFGTILDPKIREALKG (in
FT isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_040381"
FT VARIANT 148
FT /note="Q -> R (in dbSNP:rs6907125)"
FT /id="VAR_024473"
FT VARIANT 467
FT /note="I -> V (in dbSNP:rs9381594)"
FT /evidence="ECO:0000269|Ref.3"
FT /id="VAR_024474"
FT CONFLICT 427
FT /note="K -> E (in Ref. 3; ABC41928)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 708 AA; 78569 MW; 3DE541DD1D723966 CRC64;
MGLTAYGNRR VQPGELPFGA NLTLIHTRAQ PVICSKLLLT KRVSPISFFL SKFQNSWGED
GWVQLDQLPS PNAVSSDQVH CSAGCTHRKC GWAASKSKEK VPARPHGVCD GVCTDYSQCT
QPCPPDTQGN MGFSCRQKTW HKITDTCQTL NALNIFEEDS RLVQPFEDNI KISVYTGKSE
TITDMLLQKC PTDLSCVIRN IQQSPWIPGN IAVIVQLLHN ISTAIWTGVD EAKMQSYSTI
ANHILNSKSI SNWTFIPDRN SSYILLHSVN SFARRLFIDK HPVDISDVFI HTMGTTISGD
NIGKNFTFSM RINDTSNEVT GRVLISRDEL RKVPSPSQVI SIAFPTIGAI LEASLLENVT
VNGLVLSAIL PKELKRISLI FEKISKSEER RTQCVGWHSV ENRWDQQACK MIQENSQQAV
CKCRPSKLFT SFSILMSPHI LESLILTYIT YVGLGISICS LILCLSIEVL VWSQVTKTEI
TYLRHVCIVN IAATLLMADV WFIVASFLSG PITHHKGCVA ATFFVHFFYL SVFFWMLAKA
LLILYGIMIV FHTLPKSVLV ASLFSVGYGC PLAIAAITVA ATEPGKGYLR PEICWLNWDM
TKALLAFVIP ALAIVVVNLI TVTLVIVKTQ RAAIGNSMFQ EVRAIVRISK NIAILTPLLG
LTWGFGVATV IDDRSLAFHI IFSLLNAFQV SPDASDQVQS ERIHEDVL
