ESPNL_MOUSE
ID ESPNL_MOUSE Reviewed; 1005 AA.
AC Q3UYR4;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Espin-like protein;
GN Name=Espnl; Synonyms=Gm556;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP TISSUE SPECIFICITY.
RX PubMed=25582750; DOI=10.1016/j.biocel.2014.12.024;
RA Liu Q., Chen J., Gao X., Ding J., Tang Z., Zhang C., Chen J., Li L.,
RA Chen P., Wang J.;
RT "Identification of stage-specific markers during differentiation of hair
RT cells from mouse inner ear stem cells or progenitor cells in vitro.";
RL Int. J. Biochem. Cell Biol. 60:99-111(2015).
RN [3]
RP FUNCTION, INTERACTION WITH MYO3A AND MYO3B, AND SUBCELLULAR LOCATION.
RX PubMed=26926603; DOI=10.1038/ncomms10833;
RA Ebrahim S., Avenarius M.R., Grati M., Krey J.F., Windsor A.M., Sousa A.D.,
RA Ballesteros A., Cui R., Millis B.A., Salles F.T., Baird M.A.,
RA Davidson M.W., Jones S.M., Choi D., Dong L., Raval M.H., Yengo C.M.,
RA Barr-Gillespie P.G., Kachar B.;
RT "Stereocilia-staircase spacing is influenced by myosin III motors and their
RT cargos espin-1 and espin-like.";
RL Nat. Commun. 7:10833-10833(2016).
CC -!- FUNCTION: Binds to but does not cross-link actin. Required for the
CC formation and maintenance of inner ear hair cell stereocilia and
CC staircase formation. Essential for normal hearing.
CC {ECO:0000269|PubMed:26926603}.
CC -!- SUBUNIT: Interacts with MYO3A (via C-terminus). Interacts with MYO3B
CC (via C-terminus). {ECO:0000269|PubMed:26926603}.
CC -!- SUBCELLULAR LOCATION: Cell projection, stereocilium
CC {ECO:0000269|PubMed:26926603}.
CC -!- TISSUE SPECIFICITY: Expressed in inner ear hair cells
CC (PubMed:25582750). Expressed in utricle hair bundles (at protein
CC level). Expressed in choclea (at protein level) (PubMed:26926603).
CC {ECO:0000269|PubMed:25582750, ECO:0000269|PubMed:26926603}.
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DR EMBL; AK134448; BAE22148.1; -; mRNA.
DR CCDS; CCDS15160.1; -.
DR RefSeq; NP_001028464.1; NM_001033292.3.
DR AlphaFoldDB; Q3UYR4; -.
DR SMR; Q3UYR4; -.
DR STRING; 10090.ENSMUSP00000086294; -.
DR iPTMnet; Q3UYR4; -.
DR PhosphoSitePlus; Q3UYR4; -.
DR PaxDb; Q3UYR4; -.
DR PRIDE; Q3UYR4; -.
DR ProteomicsDB; 275544; -.
DR Antibodypedia; 66046; 75 antibodies from 13 providers.
DR Ensembl; ENSMUST00000088904; ENSMUSP00000086294; ENSMUSG00000049515.
DR GeneID; 227357; -.
DR KEGG; mmu:227357; -.
DR UCSC; uc007cah.1; mouse.
DR CTD; 339768; -.
DR MGI; MGI:2685402; Espnl.
DR VEuPathDB; HostDB:ENSMUSG00000049515; -.
DR eggNOG; KOG0504; Eukaryota.
DR GeneTree; ENSGT00940000156970; -.
DR HOGENOM; CLU_017253_0_0_1; -.
DR InParanoid; Q3UYR4; -.
DR OMA; MPNTDLI; -.
DR OrthoDB; 280300at2759; -.
DR PhylomeDB; Q3UYR4; -.
DR TreeFam; TF326392; -.
DR BioGRID-ORCS; 227357; 1 hit in 72 CRISPR screens.
DR PRO; PR:Q3UYR4; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q3UYR4; protein.
DR Bgee; ENSMUSG00000049515; Expressed in saccule of membranous labyrinth and 3 other tissues.
DR ExpressionAtlas; Q3UYR4; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0032426; C:stereocilium tip; IDA:UniProtKB.
DR GO; GO:0051015; F:actin filament binding; IDA:UniProtKB.
DR GO; GO:0051017; P:actin filament bundle assembly; IBA:GO_Central.
DR GO; GO:0007605; P:sensory perception of sound; IMP:UniProtKB.
DR Gene3D; 1.25.40.20; -; 3.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR Pfam; PF12796; Ank_2; 3.
DR SMART; SM00248; ANK; 9.
DR SUPFAM; SSF48403; SSF48403; 2.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 4.
PE 1: Evidence at protein level;
KW Actin-binding; ANK repeat; Cell projection; Coiled coil; Hearing;
KW Reference proteome; Repeat.
FT CHAIN 1..1005
FT /note="Espin-like protein"
FT /id="PRO_0000312512"
FT REPEAT 1..31
FT /note="ANK 1"
FT REPEAT 35..64
FT /note="ANK 2"
FT REPEAT 69..99
FT /note="ANK 3"
FT REPEAT 103..132
FT /note="ANK 4"
FT REPEAT 136..166
FT /note="ANK 5"
FT REPEAT 170..200
FT /note="ANK 6"
FT REPEAT 204..234
FT /note="ANK 7"
FT REPEAT 238..267
FT /note="ANK 8"
FT REPEAT 270..299
FT /note="ANK 9"
FT REPEAT 303..332
FT /note="ANK 10"
FT REGION 355..383
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 458..480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 611..643
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 692..729
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 764..794
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 951..975
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 502..539
FT /evidence="ECO:0000255"
SQ SEQUENCE 1005 AA; 108868 MW; 2ADDE50A7AD8FC06 CRC64;
MEAQQALVAS KDGDMATLER LFEAGALRPD ITDDLGAGLV HHATRAGHLD CVKFLVQRAK
LPGNQQAHNG ATPVHDAAAT GNLAELCWLV RDAGCGLQDQ DASGVSPLHL AARFGHPALV
EWLLREGHAA TLETLEGALP LHHAAVSGDL TCLKLLTAAH SSGVNQRTCS GASPLYLACQ
EGHLHLAQFL VKDCGADVRL RALDGMSSLH AAAAHGHYSL VVWLVTFTDI GLTARDNEGA
TALHFAARGG HTPILDRLLL MGAPIMRDSW GGTPLHDAAE NGHMECCQTL LSHHVDPFLR
DEDGYTAIDL AEYHGHQDCA QFLREMSRPV RVLMTPPPPP FPPPPLLAAK LSLEEERRGD
SGLKSPSSAT LSPVWPAQPV PREPMACTAP LRVTTPDALQ GPEVEARDSR AGLATLQLDG
LPAGDLDMLV PTQDERGRPI PEWKRQVMVR KLQARLGADH PPEDQDQSQR QDSGPTAAEQ
ATWRYSQTHQ AILGPFGELL TEDDLVYLEK QINDLQLRRR CQEYESELGR LAAQLQALLP
EPLVSITVNS HFLPRAPGLE DEEAPVLATE LEASEEPGKA EPRGQPLPFW CSHIGRLVRS
MSLLLKGMNG LAQGEEKPPS PVPQDLGKET IAGPSRSEAQ REIQECGVSV RTLRGNFEFA
PDLPCALNSG PCELGVRPGQ CLRGCWSAPP QPRGDAMGGE PGPGDTEEAS DSGISCEEAP
SEAGAGPGLD LASLRKERIV MLFLGHWKKS AYTPALRTAA CRTLEAQRAR SRGPEAAGSP
RPSSPQPSDG PRLGHLWQQR GIITHLLGTW KAIMAHVPAR QLRRLSRRER GPLSPEQFLP
HVDGAPVPYN SLSLDLFMLG YFQLLECDLP AEERKMRHLL CFEVFEHLGA HGWEAVRAFH
KAVTDEVAAG RRAWTDGFED IKARFFGSSQ GPPWDVEPGR KLGLTPLGSL PHASLPGSGP
EPAVPPRLGS DSQGSSFNSG DICGYIDRSF AFWKEKEAEM FNFGE
