ESSB_STAA8
ID ESSB_STAA8 Reviewed; 444 AA.
AC Q2G185;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 77.
DE RecName: Full=Type VII secretion system protein EssB {ECO:0000305};
GN Name=essB {ECO:0000303|PubMed:23098276};
GN OrderedLocusNames=SAOUHSC_00261 {ECO:0000312|EMBL:ABD29435.1};
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
RN [2]
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=RN6390;
RX PubMed=26785823; DOI=10.1002/1873-3468.12065;
RA Jaeger F., Zoltner M., Kneuper H., Hunter W.N., Palmer T.;
RT "Membrane interactions and self-association of components of the Ess/Type
RT VII secretion system of Staphylococcus aureus.";
RL FEBS Lett. 590:349-357(2016).
RN [3] {ECO:0007744|PDB:4ANN}
RP X-RAY CRYSTALLOGRAPHY (1.05 ANGSTROMS) OF 12-226, SUBCELLULAR LOCATION,
RP TOPOLOGY, AND SUBUNIT.
RX PubMed=23098276; DOI=10.1042/bj20121209;
RA Zoltner M., Fyfe P.K., Palmer T., Hunter W.N.;
RT "Characterization of Staphylococcus aureus EssB, an integral membrane
RT component of the TypeVII secretion system: atomic resolution crystal
RT structure of the cytoplasmic segment.";
RL Biochem. J. 449:469-477(2013).
CC -!- FUNCTION: Component of the type VII secretion system (Ess) (Probable).
CC Required for the secretion of EsxA and EsxB (By similarity).
CC {ECO:0000250|UniProtKB:P0C053, ECO:0000305}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:23098276,
CC ECO:0000269|PubMed:26785823}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23098276,
CC ECO:0000269|PubMed:26785823}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the EssB family. {ECO:0000305}.
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DR EMBL; CP000253; ABD29435.1; -; Genomic_DNA.
DR RefSeq; WP_000240338.1; NZ_LS483365.1.
DR RefSeq; YP_498855.1; NC_007795.1.
DR PDB; 4ANN; X-ray; 1.05 A; A=12-226.
DR PDBsum; 4ANN; -.
DR AlphaFoldDB; Q2G185; -.
DR SMR; Q2G185; -.
DR STRING; 1280.SAXN108_0266; -.
DR EnsemblBacteria; ABD29435; ABD29435; SAOUHSC_00261.
DR GeneID; 3919203; -.
DR KEGG; sao:SAOUHSC_00261; -.
DR PATRIC; fig|93061.5.peg.240; -.
DR eggNOG; COG4499; Bacteria.
DR HOGENOM; CLU_049737_0_0_9; -.
DR OMA; FLHYGVK; -.
DR PRO; PR:Q2G185; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 1.25.40.680; -; 1.
DR InterPro; IPR018778; T7SS_EssB.
DR InterPro; IPR042565; T7SS_EssB_C.
DR Pfam; PF10140; YukC; 1.
DR TIGRFAMs; TIGR03926; T7_EssB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Coiled coil; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..444
FT /note="Type VII secretion system protein EssB"
FT /id="PRO_0000437414"
FT TOPO_DOM 1..229
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:23098276"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..444
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:23098276"
FT REGION 366..444
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 387..443
FT /evidence="ECO:0000255"
FT COMPBIAS 368..444
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 32..36
FT /evidence="ECO:0007829|PDB:4ANN"
FT HELIX 37..39
FT /evidence="ECO:0007829|PDB:4ANN"
FT HELIX 42..52
FT /evidence="ECO:0007829|PDB:4ANN"
FT STRAND 61..65
FT /evidence="ECO:0007829|PDB:4ANN"
FT STRAND 67..74
FT /evidence="ECO:0007829|PDB:4ANN"
FT STRAND 80..82
FT /evidence="ECO:0007829|PDB:4ANN"
FT HELIX 83..88
FT /evidence="ECO:0007829|PDB:4ANN"
FT HELIX 91..100
FT /evidence="ECO:0007829|PDB:4ANN"
FT HELIX 101..109
FT /evidence="ECO:0007829|PDB:4ANN"
FT STRAND 110..113
FT /evidence="ECO:0007829|PDB:4ANN"
FT HELIX 118..120
FT /evidence="ECO:0007829|PDB:4ANN"
FT STRAND 121..123
FT /evidence="ECO:0007829|PDB:4ANN"
FT STRAND 129..132
FT /evidence="ECO:0007829|PDB:4ANN"
FT TURN 137..139
FT /evidence="ECO:0007829|PDB:4ANN"
FT HELIX 147..162
FT /evidence="ECO:0007829|PDB:4ANN"
FT HELIX 168..173
FT /evidence="ECO:0007829|PDB:4ANN"
FT HELIX 176..178
FT /evidence="ECO:0007829|PDB:4ANN"
FT HELIX 183..190
FT /evidence="ECO:0007829|PDB:4ANN"
FT HELIX 194..203
FT /evidence="ECO:0007829|PDB:4ANN"
SQ SEQUENCE 444 AA; 52024 MW; FACCFE3353DE1F77 CRC64;
MVKNHNPKNE MQDMLTPLDA EEAAKTKLRL DMREIPKSSI KPEHFHLMYL LEQHSPYFID
AELTELRDSF QIHYDINDNH TPFDNIKSFT KNEKLRYLLN IKNLEEVNRT RYTFVLAPDE
LFFTRDGLPI AKTRGLQNVV DPLPVSEAEF LTRYKALVIC AFNEKQSFDA LVEGNLELHK
GTPFETKVIE AATLDLLTAF LDEQYQKQEQ DYSQNYAYVR KVGHTVFKWV AIGMTTLSVL
LIAFLAFLYF SVMKHNERIE KGYQAFVKDD YTQVLNTYDD LDGKKLDKEA LYIYAKSYIQ
TNKQGLEKDK KENLLNNVTP NSNKDYLLYW MELGQGHLDE AINIATYLDD NDITKLALIN
KLNEIKNNGD LSNDKRSEET KKYNDKLQDI LDKEKQVKDE KAKSEEEKAK AKDEKLKQQE
ENEKKQKEQA QKDKEKRQEA ERKK
