ID   ESSD_STAA3              Reviewed;         614 AA.
AC   A0A0H2XIV9;
DT   05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Type VII secretion system protein EssD {ECO:0000305};
DE   AltName: Full=Ess-associated gene D {ECO:0000303|PubMed:21278286};
DE   AltName: Full=Nuclease toxin EssD;
GN   Name=essD {ECO:0000303|PubMed:24033479};
GN   Synonyms=esaD {ECO:0000303|PubMed:21278286};
GN   OrderedLocusNames=SAUSA300_0288 {ECO:0000312|EMBL:ABD22684.1};
OS   Staphylococcus aureus (strain USA300).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=367830;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=USA300;
RX   PubMed=16517273; DOI=10.1016/s0140-6736(06)68231-7;
RA   Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G.,
RA   Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F.,
RA   Perdreau-Remington F.;
RT   "Complete genome sequence of USA300, an epidemic clone of community-
RT   acquired meticillin-resistant Staphylococcus aureus.";
RL   Lancet 367:731-739(2006).
RN   [2]
RP   FUNCTION IN VIRULENCE, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=USA300;
RX   PubMed=21278286; DOI=10.1128/jb.01096-10;
RA   Anderson M., Chen Y.H., Butler E.K., Missiakas D.M.;
RT   "EsaD, a secretion factor for the Ess pathway in Staphylococcus aureus.";
RL   J. Bacteriol. 193:1583-1589(2011).
RN   [3]
RP   FUNCTION, GENE NAME, AND DISRUPTION PHENOTYPE.
RC   STRAIN=USA300;
RX   PubMed=24033479; DOI=10.1111/mmi.12395;
RA   Anderson M., Aly K.A., Chen Y.H., Missiakas D.;
RT   "Secretion of atypical protein substrates by the ESAT-6 secretion system of
RT   Staphylococcus aureus.";
RL   Mol. Microbiol. 90:734-743(2013).
RN   [4]
RP   INTERACTION WITH ESSE, AND FUNCTION.
RC   STRAIN=USA300;
RX   PubMed=27795322; DOI=10.1128/jb.00527-16;
RA   Anderson M., Ohr R.J., Aly K.A., Nocadello S., Kim H.K., Schneewind C.E.,
RA   Schneewind O., Missiakas D.;
RT   "EssE Promotes Staphylococcus aureus ESS-Dependent Protein Secretion To
RT   Modify Host Immune Responses during Infection.";
RL   J. Bacteriol. 199:0-0(2017).
CC   -!- FUNCTION: Component of the type VII secretion system (Ess) (Probable).
CC       Plays a role in Ess secretion during infection. Required for the
CC       efficient secretion of EsxA. Required for abscess formation and
CC       staphylococcal persistence in host tissues (PubMed:21278286,
CC       PubMed:24033479, PubMed:27795322). Possesses a toxic DNase activity
CC       that is modulated by EsaG by forming a nuclease toxin-antitoxin pair.
CC       This nuclease toxin targets competitor bacteria (By similarity).
CC       {ECO:0000250|UniProtKB:Q2G179, ECO:0000269|PubMed:21278286,
CC       ECO:0000269|PubMed:24033479, ECO:0000269|PubMed:27795322}.
CC   -!- SUBUNIT: Interacts (via C-terminal) with EssG; this interaction blocks
CC       EssD activity (By similarity). Interacts with EssE (PubMed:27795322).
CC       {ECO:0000250|UniProtKB:Q2G179, ECO:0000269|PubMed:27795322}.
CC   -!- SUBCELLULAR LOCATION: Secreted. Cell membrane
CC       {ECO:0000269|PubMed:21278286}. Note=Released from the cell in a form
CC       that is immediately active while EssG partner protein remains in the
CC       producing cell where it may potentially serve further protective
CC       functions. {ECO:0000250|UniProtKB:Q2G179}.
CC   -!- DISRUPTION PHENOTYPE: Deletions mutants are defective in the secretion
CC       of EsxA, EsxB, EsxC and EsxD. {ECO:0000269|PubMed:21278286,
CC       ECO:0000269|PubMed:24033479}.
CC   -!- SIMILARITY: Belongs to the EssD family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000255; ABD22684.1; -; Genomic_DNA.
DR   RefSeq; WP_000159025.1; NZ_CP027476.1.
DR   AlphaFoldDB; A0A0H2XIV9; -.
DR   EnsemblBacteria; ABD22684; ABD22684; SAUSA300_0288.
DR   KEGG; saa:SAUSA300_0288; -.
DR   HOGENOM; CLU_031044_1_0_9; -.
DR   OMA; NNYAQKT; -.
DR   Proteomes; UP000001939; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.40.570.10; -; 1.
DR   InterPro; IPR044929; DNA/RNA_non-sp_Endonuclease_sf.
DR   InterPro; IPR044927; Endonuclea_NS_2.
DR   InterPro; IPR027797; PT-TG_dom.
DR   Pfam; PF13930; Endonuclea_NS_2; 1.
DR   Pfam; PF14449; PT-TG; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Secreted; Virulence.
FT   CHAIN           1..614
FT                   /note="Type VII secretion system protein EssD"
FT                   /id="PRO_0000437417"
FT   REGION          417..445
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        425..439
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   614 AA;  68318 MW;  8C60D0FEEB1DC5AE CRC64;
     MTKDIEYLTA DYDNEKSSIQ SVIDAIEGQD FLDVDTTMDD AVSDVSSLDE DGAISLTSSV
     VGPQGSKLMG YYQNELYDYA SQLDSKMKEI IDTPFIEDID KAFKGITNVK LENILIKNGG
     GHGRDTYGAS GKIAKGDAKK SDSDVYSIDE ILKSDQEFVK VIDQHYKEMK KEDKKLSKSD
     FEKMMTQGAS CDYMTVAEAE ELEEQKKKEE AIEIAALAGM VVLSCINPVA GAVAIGAYSA
     YSAANAATGK NIVTGRKLSK EERIMEGLSL IPLPGMGFLK GAGKSLMKLG FKGGEKFAVK
     TGLQKTMQQA VSRISPKMGM MKNSVLNQSR NFAQNTHVGQ MLSNMRGQAT HTVQQSRNWI
     GQQAQNVKRI VNNGLDKEIA HPFKQQLAPA GMGGIKFAET TTLRNMGQNI KRAVTPQNHV
     THGPKDSMVR SEGKHSISSH EMNSSKYVES PNYTKVEFGE HYARLRPKKL KANIEYTTPT
     GHIYRTDHKG RIKEVYVDNL SLKDGDRNSH AQRTVGGEDR LPDDDGGHLI ARMFGGSKDI
     DNLVAQSKFI NRPFKEKGHW YNLEKEWQEF LNSGKEVKNI KMEVKYSGNS QRPTIFKVEY
     EINGERNIRR ILNK