ID   EST1_MESAU              Reviewed;         561 AA.
AC   Q64419;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Liver carboxylesterase;
DE            EC=3.1.1.1;
DE   Flags: Precursor;
OS   Mesocricetus auratus (Golden hamster).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC   Cricetidae; Cricetinae; Mesocricetus.
OX   NCBI_TaxID=10036;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Syrian golden; TISSUE=Liver;
RX   PubMed=8043605; DOI=10.1016/0167-4838(94)90063-9;
RA   Sone T., Isobe M., Takabatake E., Wang C.Y.;
RT   "Cloning and sequence analysis of a hamster liver cDNA encoding a novel
RT   putative carboxylesterase.";
RL   Biochim. Biophys. Acta 1207:138-142(1994).
CC   -!- FUNCTION: Involved in the detoxification of xenobiotics and in the
CC       activation of ester and amide prodrugs.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC         Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10039};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum lumen. Note=Microsomal
CC       membrane, lumen of endoplasmic reticulum.
CC   -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC       {ECO:0000305}.
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DR   EMBL; D28566; BAA05913.1; -; mRNA.
DR   PIR; S47655; S47655.
DR   RefSeq; NP_001297488.1; NM_001310559.1.
DR   AlphaFoldDB; Q64419; -.
DR   SMR; Q64419; -.
DR   ESTHER; mesau-cxest; Carb_B_Chordata.
DR   MEROPS; S09.956; -.
DR   GeneID; 106022736; -.
DR   OrthoDB; 754103at2759; -.
DR   Proteomes; UP000189706; Unplaced.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR   GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002018; CarbesteraseB.
DR   InterPro; IPR019826; Carboxylesterase_B_AS.
DR   InterPro; IPR019819; Carboxylesterase_B_CS.
DR   Pfam; PF00135; COesterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR   PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Endoplasmic reticulum; Glycoprotein; Hydrolase;
KW   Reference proteome; Serine esterase; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..561
FT                   /note="Liver carboxylesterase"
FT                   /id="PRO_0000008573"
FT   MOTIF           558..561
FT                   /note="Prevents secretion from ER"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        227
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT   ACT_SITE        345
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        459
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        276
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        362
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        95..122
FT                   /evidence="ECO:0000250"
FT   DISULFID        280..291
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   561 AA;  62331 MW;  48EA11E422475321 CRC64;
     MPLNRFPCWR YAVACGLLLL LVHVHGQDSV SPIRNTHTGQ VRGKLVYVKE GVTGVYAFLG
     IPFAKPPVGP LRFAPPEPPE PWSGVRDGTS EPAMCLQTDF MRPQISKERK IILPTISMSE
     DCLYLNIYTP AHAHEGSNLP VMVWIHGGAL VMGMASMNDG SLLAATEDIV IVSIQYRLGI
     LGFFSTGDEH ARGNWGYLDQ VAALHWVQQN IASFGGNPGQ VTIFGVSAGG TSVSSLVVSP
     MSKGLFHGAI MQSGVALLPD LISDTPEAVY TPVVANQSGC EAKDSEALVH CLREKTEAEI
     LAINQVFIMT PGVVDGIFLP RHPQELLASV DFHPVPSIIG VDSDECGWGV PLFMGLDHVI
     KNITRETLPA FLKSRAEHMM LPPECSDLLM QEYMGDVEDP QTLQAQFREL MKDFMFVIPA
     LKVAYFQRSH APVYFYEFQH QSSFIKNKDA RPSHVRADHG DHVAFVFGSD FWGLKIDLTE
     EEKLLNKRMM KYWANFARHG NPNSEGLPYW PELVHDDQYL KLDIQPAVGR ALKSRKLHFW
     TKILPQKIQE LKGAQGKHSE L