ID   EST1_PSEFL              Reviewed;         218 AA.
AC   Q51758;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Carboxylesterase 1;
DE            EC=3.1.1.1;
DE   AltName: Full=Esterase I;
GN   Name=estA;
OS   Pseudomonas fluorescens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=294;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Yu F.;
RL   Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Hydrolyzes carboxylic ester bonds with relatively broad
CC       substrate specificity.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC         Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. AB hydrolase 2
CC       family. {ECO:0000305}.
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DR   EMBL; D00852; BAA00727.1; -; Genomic_DNA.
DR   PIR; JQ0885; JQ0885.
DR   AlphaFoldDB; Q51758; -.
DR   SMR; Q51758; -.
DR   ESTHER; psefl-cxes1; LYsophospholipase_carboxylesterase.
DR   GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR003140; PLipase/COase/thioEstase.
DR   Pfam; PF02230; Abhydrolase_2; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Serine esterase.
FT   CHAIN           1..218
FT                   /note="Carboxylesterase 1"
FT                   /id="PRO_0000102274"
FT   ACT_SITE        114
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        168
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        199
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   218 AA;  24153 MW;  E61AE76AFFFD6E6D CRC64;
     MTEPLILQPA KPADACVIWL HGLGADRYDF LPVAEALQET LLSTRFVLPQ APTRPVTING
     GYEMPSWYDI KAMSPARSIS LEELETSAKT VTDLIETQQR TGIDTSRIFL AGFSQGGAVV
     FHTAFKKWEG PLGGVIALST YAPTFDNDLQ LSASQQRIPT LCLHGQYDEV VQNAMGRSAY
     EHLKGRGVTV TWQEYPMGHE VLPQEIHDIG AWLAERLR