ID   EST2_CULPI              Reviewed;         215 AA.
AC   P21370;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Esterase B2;
DE            EC=3.1.1.1;
DE   Flags: Fragment;
OS   Culex pipiens (House mosquito).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Culicinae; Culicini; Culex; Culex.
OX   NCBI_TaxID=7175;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=2005964; DOI=10.1038/350151a0;
RA   Raymond M., Callaghan A., Fort P., Pasteur N.;
RT   "Worldwide migration of amplified insecticide resistance genes in
RT   mosquitoes.";
RL   Nature 350:151-153(1991).
CC   -!- FUNCTION: Overproduction of nonspecific esterases is a common mechanism
CC       of resistance to organophosphate insecticides.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC         Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10039};
CC   -!- MISCELLANEOUS: There are two such esterases: A and B. Alleles of both A
CC       and B are known.
CC   -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC       {ECO:0000305}.
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DR   PIR; S14287; S14287.
DR   AlphaFoldDB; P21370; -.
DR   SMR; P21370; -.
DR   GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002018; CarbesteraseB.
DR   Pfam; PF00135; COesterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Hydrolase; Serine esterase.
FT   CHAIN           <1..>215
FT                   /note="Esterase B2"
FT                   /id="PRO_0000070273"
FT   ACT_SITE        56
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        184
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   NON_TER         1
FT   NON_TER         215
SQ   SEQUENCE   215 AA;  24937 MW;  5DA120EE82A52F3D CRC64;
     KLLTDQDMQD DIFTPFGPTV EPYLTEQCMI PKEPFEMART AWGDRIDIMI GGTSEEGLLL
     LQKIKLHPEL LSHPHLFLGN VPPNLKISME KRIEFAAKLK QRYYPDSSPS MENNLGYVHM
     MSDRVFWHGL HRTILARGAR SRARTFVYRI CLDSEFYNHY RIMMIDPKLR GTAHADELSY
     LFSNFTQQVP GKETFEYRGL QTLVDVFTAF VINGD