ID   EST4A_BOVIN             Reviewed;         550 AA.
AC   P0C6R3;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Carboxylesterase 4A;
DE            EC=3.1.1.-;
DE   Flags: Precursor;
GN   Name=CES4A; Synonyms=CES8;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal skin;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Probable carboxylesterase. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC       {ECO:0000305}.
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DR   EMBL; BC149217; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; NP_001161306.1; NM_001167834.1.
DR   AlphaFoldDB; P0C6R3; -.
DR   SMR; P0C6R3; -.
DR   STRING; 9913.ENSBTAP00000050404; -.
DR   ESTHER; bovin-est8; Carb_B_Chordata.
DR   PaxDb; P0C6R3; -.
DR   Ensembl; ENSBTAT00000052032; ENSBTAP00000050404; ENSBTAG00000038325.
DR   GeneID; 529706; -.
DR   KEGG; bta:529706; -.
DR   CTD; 283848; -.
DR   VEuPathDB; HostDB:ENSBTAG00000038325; -.
DR   VGNC; VGNC:27231; CES4A.
DR   eggNOG; KOG1516; Eukaryota.
DR   GeneTree; ENSGT00940000162924; -.
DR   HOGENOM; CLU_006586_13_0_1; -.
DR   InParanoid; P0C6R3; -.
DR   OrthoDB; 754103at2759; -.
DR   TreeFam; TF315470; -.
DR   Proteomes; UP000009136; Chromosome 18.
DR   Bgee; ENSBTAG00000038325; Expressed in zone of skin and 42 other tissues.
DR   ExpressionAtlas; P0C6R3; baseline.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002018; CarbesteraseB.
DR   InterPro; IPR019826; Carboxylesterase_B_AS.
DR   Pfam; PF00135; COesterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Hydrolase; Reference proteome; Secreted;
KW   Serine esterase; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..550
FT                   /note="Carboxylesterase 4A"
FT                   /id="PRO_0000325922"
FT   ACT_SITE        221
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT   ACT_SITE        353
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        465
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        276
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        386
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        88..116
FT                   /evidence="ECO:0000250"
FT   DISULFID        273..284
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   550 AA;  61777 MW;  EBB5DC98512A5D87 CRC64;
     MNWILCLSLT LLLVVQTAWG ALHTKEPLAD TKYGTLRGKQ IHVGKTPINV FLGVPFSKPP
     VGAHRFAAPE PPEPWEGIRD ATTYAPVCLQ ESWGQVTSMY FNTHKRYKWL HFSEDCLYLN
     VHAPVRARGD PLQPVMIWFP GGAFLVGSAS TYDGSELAAR EKVVVVVLQH RLGILGFLST
     GDSQARGNWA LLDQIAALRW VQKNIEAFGG DPGCVTLFGQ SSGAMCISGL MTSSLARGLF
     HRAISQSGTA VFQNFITPDP LKVAKKIAQL AGCNHNSTKI LVDCLRTLSG AEVMRVSQKM
     RFFKLHSQED PQKVVWFMSP VVDGVVFQDN PIVLLTQGQV APVPYLLGVN NLEFNWLLPF
     IMKFPMSHLR KETIAKLLWS TSTLLNVTKE QLPLVMEEYL RDVDDHDQKM LQKHVMDLAA
     DATFVYSTLQ AAHYHHNAGF PVYLYEFEHY APGTIVKPRT DGADHGDEIG FIFGSPFSKG
     HSSNKEKALS LQMMKYWANF ARTGNPNGGK LPYWPRYNKD EKYLQLDLTT RVGVMLREEK
     MAFWKRLHQN