EST4A_MOUSE
ID EST4A_MOUSE Reviewed; 563 AA.
AC Q8R0W5; G3XA24;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Carboxylesterase 4A;
DE EC=3.1.1.-;
DE Flags: Precursor;
GN Name=Ces4a; Synonyms=Ces8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probable carboxylesterase. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH26374.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC164311; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466525; EDL11247.1; -; Genomic_DNA.
DR EMBL; BC026374; AAH26374.1; ALT_INIT; mRNA.
DR CCDS; CCDS22591.2; -.
DR RefSeq; NP_666325.2; NM_146213.2.
DR AlphaFoldDB; Q8R0W5; -.
DR SMR; Q8R0W5; -.
DR STRING; 10090.ENSMUSP00000125062; -.
DR ESTHER; mouse-Ces4a; Carb_B_Chordata.
DR MEROPS; S09.962; -.
DR GlyGen; Q8R0W5; 3 sites.
DR iPTMnet; Q8R0W5; -.
DR PhosphoSitePlus; Q8R0W5; -.
DR PaxDb; Q8R0W5; -.
DR PeptideAtlas; Q8R0W5; -.
DR PRIDE; Q8R0W5; -.
DR ProteomicsDB; 275690; -.
DR Antibodypedia; 48401; 76 antibodies from 14 providers.
DR DNASU; 234677; -.
DR Ensembl; ENSMUST00000161289; ENSMUSP00000125062; ENSMUSG00000060560.
DR GeneID; 234677; -.
DR KEGG; mmu:234677; -.
DR UCSC; uc009nbo.1; mouse.
DR CTD; 283848; -.
DR MGI; MGI:2384581; Ces4a.
DR VEuPathDB; HostDB:ENSMUSG00000060560; -.
DR eggNOG; KOG1516; Eukaryota.
DR GeneTree; ENSGT00940000162924; -.
DR HOGENOM; CLU_006586_13_0_1; -.
DR InParanoid; Q8R0W5; -.
DR OMA; QESWGQI; -.
DR OrthoDB; 754103at2759; -.
DR PhylomeDB; Q8R0W5; -.
DR TreeFam; TF315470; -.
DR BioGRID-ORCS; 234677; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Ces4a; mouse.
DR PRO; PR:Q8R0W5; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8R0W5; protein.
DR Bgee; ENSMUSG00000060560; Expressed in tail skin and 14 other tissues.
DR Genevisible; Q8R0W5; MM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002018; CarbesteraseB.
DR InterPro; IPR019826; Carboxylesterase_B_AS.
DR InterPro; IPR019819; Carboxylesterase_B_CS.
DR Pfam; PF00135; COesterase; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Hydrolase; Reference proteome; Secreted;
KW Serine esterase; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..563
FT /note="Carboxylesterase 4A"
FT /id="PRO_0000325924"
FT ACT_SITE 228
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT ACT_SITE 360
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 475
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT CARBOHYD 283
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 379
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 395
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 95..123
FT /evidence="ECO:0000250"
FT DISULFID 280..291
FT /evidence="ECO:0000250"
SQ SEQUENCE 563 AA; 62883 MW; 5C0DFAC6CF1366E2 CRC64;
MAEAGWNMKW ILGLSLTLCL VVQTALGALH TKEPLLITKH GILQGKQVHV GDTPIQVFLG
IPFSKPPVGT RRFAPPEPPL PWNGIRDATT YPPSCLQESW GQILSMYLNT RKQYKWLHFS
EDCLYLNVYA PVLAPGAPLL PVMVWFPGGA FLAGSASTYE GSELAARGKV VLVFLQYRLG
ILGFFSTGNS HARGNWGLLD QIAALRWVQE NIEAFGGDPD SVTLFGQSAG AMSVSGLMVS
PLAQGLFHQA ISQSGTAVLK AFITPDPLTS AKKIAHLAGC DHNSTKVMVE CLRSLSAEEV
TLVSKRMSFF QANSHKDPKE IVWFLSPVVD GVVFPEDPVV LLTRGQVKPV PYLLGVNNAE
FEWNLPFLMK IQLNQRMMNQ TNINKILWSF NVLLNITKEQ VPVVAREYLN DAISNHDWKM
FRNRLIDLIG DATFVYATLQ AARYHRDAGS PVYLYEFKHH ASSGIIIKPY NGADHGDELS
YLFGSPFSKG SSAGEEKEFS LQMMKYWANF AHTGNPNGEK LPYWPRFDKD EKYLQLDFDT
RVGVKLKEKK MAFWSQLRQS QKP
