ID   EST5A_DROMI             Reviewed;         555 AA.
AC   O16168;
DT   23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Esterase-5A;
DE            Short=Est-5A;
DE            EC=3.1.1.1;
DE   AltName: Full=Carboxylic-ester hydrolase 5A;
DE            Short=Carboxylesterase-5A;
DE   Flags: Precursor;
GN   Name=Est-5A; Synonyms=Est5A;
OS   Drosophila miranda (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7229;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9475741; DOI=10.1093/genetics/148.1.305;
RA   King L.M.;
RT   "The role of gene conversion in determining sequence variation and
RT   divergence in the Est-5 gene family in Drosophila pseudoobscura.";
RL   Genetics 148:305-315(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC         Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10039};
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF016108; AAB70219.1; -; Genomic_DNA.
DR   AlphaFoldDB; O16168; -.
DR   SMR; O16168; -.
DR   ESTHER; dromi-est5a; Carb_B_Arthropoda.
DR   MEROPS; S09.947; -.
DR   FlyBase; FBgn0021338; Dmir\Est-5A.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002018; CarbesteraseB.
DR   InterPro; IPR019826; Carboxylesterase_B_AS.
DR   InterPro; IPR019819; Carboxylesterase_B_CS.
DR   Pfam; PF00135; COesterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR   PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Glycoprotein; Hydrolase; Secreted; Serine esterase; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..555
FT                   /note="Esterase-5A"
FT                   /id="PRO_0000008552"
FT   ACT_SITE        210
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT   CARBOHYD        95
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        116
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        479
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        510
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        87..106
FT                   /evidence="ECO:0000250"
FT   DISULFID        262..274
FT                   /evidence="ECO:0000250"
FT   DISULFID        518..539
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   555 AA;  62282 MW;  E8EACEA4AE379630 CRC64;
     MHLVRWLICL IQLWIQLGAA GSVTLLDPLL IEIPNGKLRG RDNGHYYSYE AIPYAEPPTG
     ELRFEVPKPY KQQWTNTFDA TQPPVLCMQW NQFINGTNKL LGVEDCLTVS VYRPKNSSRN
     NFPVVANLHG GAFMFGGPSQ YGHENIMREG SVILVTIGYR LGPLGFVSTG DADLSGNFGL
     KDQRLALLWI KQNIASFGGE PENILVVGHS AGGASVHLQM LREDFSKVAK AAISFSGNAL
     DPWVIQQGLR GRTFELGRIV GCGQASDSVT LKKCLKSKPA SEIVSAVQSF LVFSYVPFTP
     FGPAIESPDA PEAFITQHPI DIIKSGKFAQ VPWAVTYTTE DGGYNAALLL EKQASSGREL
     ILDLNDRWFD WAPYLLFYRD SMTTIKDMDD YSRKLRQEYL GDRRFSVESY WDVQRMFTDL
     LFKNSVTVSV DLHRKYGKSP VYAFVYDNPA EVGVGQILSG RNDVYFGTVH GDDVFLIFNV
     SFVPANRRPD EQIISRNFIK MLEYFALSTN DTMAYGDCVF QNNVGSKHMQ LLSITRDGCE
     NKQLNCFIQR CLIFF