EST5A_DROPS
ID EST5A_DROPS Reviewed; 548 AA.
AC P25727; B5DQP1; O16174; O16175; O16176; O16177; O16178; O16179; O18663;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 3.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=Esterase-5A;
DE Short=Est-5A;
DE EC=3.1.1.1;
DE AltName: Full=Carboxylic-ester hydrolase 5A;
DE Short=Carboxylesterase-5A;
DE Flags: Precursor;
GN Name=Est-5A; Synonyms=Est5A; ORFNames=GA23705;
OS Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=46245;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS ILE-15; DEL-26; GLY-139;
RP THR-226; VAL-228 AND VAL-269.
RX PubMed=2178209; DOI=10.1093/oxfordjournals.molbev.a040624;
RA Brady J.P., Richmond R.C., Oakeshott J.G.;
RT "Cloning of the esterase-5 locus from Drosophila pseudoobscura and
RT comparison with its homologue in D. melanogaster.";
RL Mol. Biol. Evol. 7:525-546(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS ILE-15; DEL-26; GLY-139;
RP THR-226; VAL-228; LEU-260; ASN-266; LEU-269; VAL-269; SER-281; THR-307;
RP MET-327; LYS-330; THR-355; PHE-442; ASP-451; HIS-456; SER-487 AND PHE-546.
RC STRAIN=GB115E, GB139E, GB336E, GB4E, GB8E, JR198E, JR341E, and JR50E;
RX PubMed=9475741; DOI=10.1093/genetics/148.1.305;
RA King L.M.;
RT "The role of gene conversion in determining sequence variation and
RT divergence in the Est-5 gene family in Drosophila pseudoobscura.";
RL Genetics 148:305-315(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MV2-25 / Tucson 14011-0121.94;
RX PubMed=15632085; DOI=10.1101/gr.3059305;
RA Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA Weinstock G.M., Gibbs R.A.;
RT "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT gene, and cis-element evolution.";
RL Genome Res. 15:1-18(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10039};
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EDY73464.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; M55908; AAA28516.1; -; Genomic_DNA.
DR EMBL; AF016135; AAB70225.1; -; Genomic_DNA.
DR EMBL; AF016136; AAB70226.1; -; Genomic_DNA.
DR EMBL; AF016137; AAB70227.1; -; Genomic_DNA.
DR EMBL; AF016138; AAB70228.1; -; Genomic_DNA.
DR EMBL; AF016139; AAB70229.1; -; Genomic_DNA.
DR EMBL; AF016140; AAB70230.1; -; Genomic_DNA.
DR EMBL; AF016141; AAB70231.1; -; Genomic_DNA.
DR EMBL; AF016142; AAB70232.1; -; Genomic_DNA.
DR EMBL; CH379069; EDY73464.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; P25727; -.
DR SMR; P25727; -.
DR STRING; 46245.P25727; -.
DR ESTHER; drops-est5a; Carb_B_Arthropoda.
DR MEROPS; S09.947; -.
DR eggNOG; KOG1516; Eukaryota.
DR InParanoid; P25727; -.
DR Proteomes; UP000001819; Genome assembly.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002018; CarbesteraseB.
DR InterPro; IPR019826; Carboxylesterase_B_AS.
DR InterPro; IPR019819; Carboxylesterase_B_CS.
DR Pfam; PF00135; COesterase; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Glycoprotein; Hydrolase; Reference proteome; Secreted;
KW Serine esterase; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..548
FT /note="Esterase-5A"
FT /id="PRO_0000008554"
FT ACT_SITE 210
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT CARBOHYD 95
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 116
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 479
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 87..106
FT /evidence="ECO:0000250"
FT DISULFID 262..274
FT /evidence="ECO:0000250"
FT DISULFID 518..539
FT /evidence="ECO:0000255"
FT VARIANT 15
FT /note="V -> I (in strain: GB4E, GB8E, GB139E and GB336E)"
FT /evidence="ECO:0000269|PubMed:2178209,
FT ECO:0000269|PubMed:9475741"
FT VARIANT 26
FT /note="Missing (in strain: GB4E, GB8E, GB139E and GB336E)"
FT VARIANT 139
FT /note="S -> G (in strain: GB4E, GB8E, GB115E, GB139E,
FT GB336E, JR198E and JR341E)"
FT /evidence="ECO:0000269|PubMed:2178209,
FT ECO:0000269|PubMed:9475741"
FT VARIANT 226
FT /note="S -> T (in strain: GB4E, GB8E, GB139E, JR50E and
FT JR198E)"
FT /evidence="ECO:0000269|PubMed:2178209,
FT ECO:0000269|PubMed:9475741"
FT VARIANT 228
FT /note="L -> V (in strain: GB4E, GB8E, GB115E, GB139E,
FT GB336E, JR50E and JR198E)"
FT /evidence="ECO:0000269|PubMed:2178209,
FT ECO:0000269|PubMed:9475741"
FT VARIANT 260
FT /note="V -> L (in strain: GB336E)"
FT /evidence="ECO:0000269|PubMed:9475741"
FT VARIANT 266
FT /note="S -> N (in strain: JR341E)"
FT /evidence="ECO:0000269|PubMed:9475741"
FT VARIANT 269
FT /note="G -> L (in strain: GB336E)"
FT /evidence="ECO:0000269|PubMed:9475741"
FT VARIANT 269
FT /note="G -> V (in strain: GB4E, GB8E, GB115E, GB139E,
FT JR198E and JR341E)"
FT /evidence="ECO:0000269|PubMed:2178209,
FT ECO:0000269|PubMed:9475741"
FT VARIANT 281
FT /note="I -> S (in strain: JR341E)"
FT /evidence="ECO:0000269|PubMed:9475741"
FT VARIANT 307
FT /note="S -> T (in strain: GB115E)"
FT /evidence="ECO:0000269|PubMed:9475741"
FT VARIANT 327
FT /note="K -> M (in strain: JR198E)"
FT /evidence="ECO:0000269|PubMed:9475741"
FT VARIANT 330
FT /note="Q -> K (in strain: JR198E)"
FT /evidence="ECO:0000269|PubMed:9475741"
FT VARIANT 355
FT /note="S -> T (in strain: GB139E)"
FT /evidence="ECO:0000269|PubMed:9475741"
FT VARIANT 442
FT /note="Y -> F (in strain: JR341E)"
FT /evidence="ECO:0000269|PubMed:9475741"
FT VARIANT 451
FT /note="E -> D (in strain: JR341E)"
FT /evidence="ECO:0000269|PubMed:9475741"
FT VARIANT 456
FT /note="Q -> H (in strain: JR198E)"
FT /evidence="ECO:0000269|PubMed:9475741"
FT VARIANT 487
FT /note="R -> S (in strain: GB336E)"
FT /evidence="ECO:0000269|PubMed:9475741"
FT VARIANT 546
FT /note="C -> F (in strain: GB139E and JR50E)"
FT /evidence="ECO:0000269|PubMed:9475741"
FT CONFLICT 334
FT /note="A -> L (in Ref. 1; AAA28516)"
FT /evidence="ECO:0000305"
FT CONFLICT 467..470
FT /note="GTVH -> D (in Ref. 1; AAA28516)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 548 AA; 61376 MW; AA16F36ED13D0B52 CRC64;
MHLVRWLICL IQLWVQLGAA GSVTLLDPLL IEIPNGKLRG RDNGHYYSYE AIPYAEPPTG
ELRFEVPKPY KQQWTNTFDA TQPPVLCMQW NQFINGTNKL LGVEDCLTVS VYRPKNSSRN
NFPVVANLHG GAFMFGGPSQ YGHENIMREG SVILVTIGYR LGPLGFVSTG DADLSGNFGL
KDQRLALLWI KQNIASFGGE PENILVVGHS AGGASVHLQM LREDFSKLAK AAISFSGNAL
DPWVIQQGLR GRAFELGRIV GCGQASDSGT LKKCLKSKPA IEIVSAVRSF LVFSYVPFTP
FGPAIESPDA PEAFITQHPI DIIKSGKFSQ VPWAVTYTTE DGGYNAALLL EKQASSGREL
IVDLNDRWFD WAPYLLFYRD SMTTIKDMDD YSRKLRQEYL GDRRFSVESY WDVQRMFTDL
LFKNSVTVSV DLHRKYGKSP VYAFVYDNPS EVGVGQILSG RNDVYFGTVH GDDVFLIFNV
SFVPANRRPD EEIISRNFIK MLEYFALSTD DTMAYGDCVF QNNVGSKHMQ LLSITRDGCE
NKQLNCFI
