EST5A_FELCA
ID EST5A_FELCA Reviewed; 545 AA.
AC Q8I034;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Carboxylesterase 5A;
DE EC=3.1.1.1;
DE AltName: Full=Carboxylesterase-like urinary excreted protein;
DE Short=Cauxin;
DE Flags: Precursor;
GN Name=CES5A; Synonyms=CES7;
OS Felis catus (Cat) (Felis silvestris catus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Feliformia; Felidae; Felinae; Felis.
OX NCBI_TaxID=9685;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-57; 93-114; 151-172;
RP 248-264; 280-297; 308-330 AND 533-541, BIOPHYSICOCHEMICAL PROPERTIES,
RP GLYCOSYLATION, AND TISSUE SPECIFICITY.
RC TISSUE=Kidney;
RX PubMed=12401131; DOI=10.1042/bj20021446;
RA Miyazaki M., Kamiie K., Soeta S., Taira H., Yamashita T.;
RT "Molecular cloning and characterization of a novel carboxylesterase-like
RT protein that is physiologically present at high concentrations in the urine
RT of domestic cats (Felis catus).";
RL Biochem. J. 370:101-110(2003).
RN [2]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=17045831; DOI=10.1016/j.cbpb.2006.05.015;
RA Miyazaki M., Yamashita T., Hosokawa M., Taira H., Suzuki A.;
RT "Species-, sex-, and age-dependent urinary excretion of cauxin, a mammalian
RT carboxylesterase.";
RL Comp. Biochem. Physiol. 145B:270-277(2006).
RN [3]
RP FUNCTION.
RX PubMed=17052611; DOI=10.1016/j.chembiol.2006.08.013;
RA Miyazaki M., Yamashita T., Suzuki Y., Saito Y., Soeta S., Taira H.,
RA Suzuki A.;
RT "A major urinary protein of the domestic cat regulates the production of
RT felinine, a putative pheromone precursor.";
RL Chem. Biol. 13:1071-1079(2006).
RN [4]
RP GLYCOSYLATION AT ASN-86.
RX PubMed=17341822; DOI=10.1271/bbb.60599;
RA Suzuki Y., Miyazaki M., Ito E., Suzuki M., Yamashita T., Taira H.,
RA Suzuki A.;
RT "Structural characterization of N-glycans of cauxin by MALDI-TOF mass
RT spectrometry and nano LC-ESI-mass spectrometry.";
RL Biosci. Biotechnol. Biochem. 71:811-816(2007).
RN [5]
RP INDUCTION.
RX PubMed=16919690; DOI=10.1016/j.rvsc.2006.06.009;
RA Miyazaki M., Soeta S., Yamagishi N., Taira H., Suzuki A., Yamashita T.;
RT "Tubulointerstitial nephritis causes decreased renal expression and urinary
RT excretion of cauxin, a major urinary protein of the domestic cat.";
RL Res. Vet. Sci. 82:76-79(2007).
CC -!- FUNCTION: Carboxylesterase present at high level in urine that
CC regulates production of felinine, a probable pheromone precursor.
CC Probably acts by hydrolyzing the peptide bond of the felinine precursor
CC 3-methylbutanol cyteinylglycine, producing felinine and glycine in cat
CC urine. {ECO:0000269|PubMed:17052611}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10039};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=506 uM for p-NPA {ECO:0000269|PubMed:12401131};
CC Vmax=7.61 umol/min/mg enzyme with p-NPA as substrate
CC {ECO:0000269|PubMed:12401131};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Present at high level in urine. Expressed in the
CC kidney proximal straight tubular cells and is secreted from the apical
CC compartment of the cells into the urine (at protein level). In mature
CC cats, it is present at higher level in intact males than in castrated
CC males or in intact or spayed females. {ECO:0000269|PubMed:12401131,
CC ECO:0000269|PubMed:17045831}.
CC -!- DEVELOPMENTAL STAGE: Present in cats older than about 3 months, and its
CC level increases with age. {ECO:0000269|PubMed:17045831}.
CC -!- INDUCTION: Strongly down-regulated in cats suffering from
CC tubulointerstitial nephritis (TIN). Excretion decreases immediately
CC after castration. {ECO:0000269|PubMed:16919690,
CC ECO:0000269|PubMed:17045831}.
CC -!- PTM: N-glycosylated; contains a fucosylated complex carbohydrate.
CC {ECO:0000269|PubMed:12401131, ECO:0000269|PubMed:17341822}.
CC -!- MISCELLANEOUS: Protein specifically present in urine of cat. Present at
CC high level in urine of members of the genus Felis and Lynx but not in
CC urine of other felines.
CC -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC {ECO:0000305}.
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DR EMBL; AB045377; BAC22577.1; -; mRNA.
DR RefSeq; NP_001009188.1; NM_001009188.1.
DR AlphaFoldDB; Q8I034; -.
DR SMR; Q8I034; -.
DR STRING; 9685.ENSFCAP00000013286; -.
DR ESTHER; felca-CAUXIN; Carb_B_Chordata.
DR iPTMnet; Q8I034; -.
DR PRIDE; Q8I034; -.
DR GeneID; 445455; -.
DR KEGG; fca:445455; -.
DR CTD; 221223; -.
DR eggNOG; KOG1516; Eukaryota.
DR InParanoid; Q8I034; -.
DR OrthoDB; 754103at2759; -.
DR BioCyc; MetaCyc:MON-20541; -.
DR SABIO-RK; Q8I034; -.
DR Proteomes; UP000011712; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002018; CarbesteraseB.
DR InterPro; IPR019826; Carboxylesterase_B_AS.
DR InterPro; IPR019819; Carboxylesterase_B_CS.
DR Pfam; PF00135; COesterase; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Glycoprotein; Hydrolase;
KW Reference proteome; Secreted; Serine esterase; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000269|PubMed:12401131"
FT CHAIN 29..545
FT /note="Carboxylesterase 5A"
FT /id="PRO_0000308590"
FT ACT_SITE 226
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT ACT_SITE 346
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 454
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT CARBOHYD 86
FT /note="N-linked (GlcNAc...) (complex) asparagine"
FT /evidence="ECO:0000269|PubMed:17341822"
FT CARBOHYD 134
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 363
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 443
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 94..121
FT /evidence="ECO:0000250"
FT DISULFID 281..292
FT /evidence="ECO:0000250"
SQ SEQUENCE 545 AA; 60506 MW; 9F73FA693D271FA9 CRC64;
MSGMWVHPGR TLIWALWVLA AVIKGPAADA PVRSTRLGWV RGKQTTVLGS TVPVNMFLGI
PYAAPPLGPL RFKQPKPALP GNDFRNATSY PKLCFQDLEW LVSYQHVLKV RYPKLEASED
CLYLNIYAPA HADNGSNLPV MVWFPGGAFK MGSASSFDGS ALAAYEDVLI VTTQYRLGIF
GFFDTGDEHA RGNWALLDQV AALTWVRDNI EFFGGDPRSV TIFGESAGAI SVSSLILSPI
ANGLFHKAIM ESGVAILPLL MRPPGDERKK DLQVLARICG CHASDSAALL QCLRAKPSEE
LMDISKKLTF SIPVIDDFFF PDEPVALLTQ KAFNSVPSII GVNNHECAFL LSTEFSEILG
GSNRSLALYL VHTFLNIPTQ YLHLVADHYF YNKHSPVEIR DSFLDLLGDV LFVVPGVVTA
RYHRDAGAPV YFYEFQHPPQ CLNDTRPAFV KADHSDEIRF VFGGAFLKGD IVMFEGATEE
EKLLSRKMMR YWANFARTGD PNGEGVPLWP AYTQSEQYLK LDLSVSVGQK LKEQEVEFWM
NTIVP
