EST5B_DROPS
ID EST5B_DROPS Reviewed; 545 AA.
AC P25726; Q27614; Q27615; Q27616; Q27617; Q27618; Q27619; Q27620; Q27621;
AC Q27622; Q27623; Q27624; Q27625; Q27626; Q27627; Q27628; Q27629;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 2.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Esterase-5B;
DE Short=Est-5B;
DE EC=3.1.1.1;
DE AltName: Full=Carboxylic-ester hydrolase 5B;
DE Short=Carboxylesterase-5B;
DE Flags: Precursor;
GN Name=Est-5B; Synonyms=Est5b; ORFNames=GA14349;
OS Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=46245;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2178209; DOI=10.1093/oxfordjournals.molbev.a040624;
RA Brady J.P., Richmond R.C., Oakeshott J.G.;
RT "Cloning of the esterase-5 locus from Drosophila pseudoobscura and
RT comparison with its homologue in D. melanogaster.";
RL Mol. Biol. Evol. 7:525-546(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=GB-115, GB-139, GB-329, GB-336, GB-4, GB-8, JR-106, JR-126, JR-142,
RC JR-198, JR-32, JR-341, JR-38, JR-382, JR-460, and JR-50;
RX PubMed=8536974; DOI=10.1093/genetics/141.1.255;
RA Veuille M., King L.M.;
RT "Molecular bases of polymorphism at the esterase-5B locus in Drosophila
RT pseudoobscura.";
RL Genetics 141:255-262(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MV2-25 / Tucson 14011-0121.94;
RX PubMed=15632085; DOI=10.1101/gr.3059305;
RA Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA Weinstock G.M., Gibbs R.A.;
RT "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT gene, and cis-element evolution.";
RL Genome Res. 15:1-18(2005).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10039};
CC -!- SUBUNIT: Homodimer.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Eye and hemolymph.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development.
CC -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC {ECO:0000305}.
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DR EMBL; M55907; AAA28515.1; -; Genomic_DNA.
DR EMBL; X89085; CAA61457.1; -; Genomic_DNA.
DR EMBL; X89086; CAA61458.1; -; Genomic_DNA.
DR EMBL; X89087; CAA61459.1; -; Genomic_DNA.
DR EMBL; X89088; CAA61460.1; -; Genomic_DNA.
DR EMBL; X89089; CAA61461.1; -; Genomic_DNA.
DR EMBL; X89090; CAA61462.1; -; Genomic_DNA.
DR EMBL; X89091; CAA61463.1; -; Genomic_DNA.
DR EMBL; X89092; CAA61464.1; -; Genomic_DNA.
DR EMBL; X89093; CAA61465.1; -; Genomic_DNA.
DR EMBL; X89094; CAA61466.1; -; Genomic_DNA.
DR EMBL; X89095; CAA61467.1; -; Genomic_DNA.
DR EMBL; X89096; CAA61468.1; -; Genomic_DNA.
DR EMBL; X89097; CAA61469.1; -; Genomic_DNA.
DR EMBL; X89098; CAA61470.1; -; Genomic_DNA.
DR EMBL; X89099; CAA61471.1; -; Genomic_DNA.
DR EMBL; X89100; CAA61472.1; -; Genomic_DNA.
DR EMBL; CH379069; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S58968; S58968.
DR PIR; S58969; S58969.
DR PIR; S58970; S58970.
DR PIR; S58971; S58971.
DR PIR; S58972; S58972.
DR PIR; S58973; S58973.
DR PIR; S58974; S58974.
DR PIR; S58975; S58975.
DR PIR; S58976; S58976.
DR PIR; S58977; S58977.
DR PIR; S58978; S58978.
DR PIR; S58979; S58979.
DR PIR; S58980; S58980.
DR PIR; S58981; S58981.
DR PIR; S58982; S58982.
DR PIR; S58983; S58983.
DR AlphaFoldDB; P25726; -.
DR SMR; P25726; -.
DR STRING; 7237.FBpp0274978; -.
DR ESTHER; drops-est5b; Carb_B_Arthropoda.
DR MEROPS; S09.947; -.
DR eggNOG; KOG1516; Eukaryota.
DR InParanoid; P25726; -.
DR Proteomes; UP000001819; Genome assembly.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002018; CarbesteraseB.
DR InterPro; IPR019826; Carboxylesterase_B_AS.
DR InterPro; IPR019819; Carboxylesterase_B_CS.
DR Pfam; PF00135; COesterase; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Hydrolase; Reference proteome; Secreted;
KW Serine esterase; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..545
FT /note="Esterase-5B"
FT /id="PRO_0000008557"
FT ACT_SITE 207
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT ACT_SITE 467
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT CARBOHYD 113
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 421
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 507
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 84..103
FT /evidence="ECO:0000250"
FT DISULFID 259..271
FT /evidence="ECO:0000250"
FT DISULFID 515..536
FT /evidence="ECO:0000255"
FT VARIANT 4
FT /note="A -> T (in strain: JR-341)"
FT VARIANT 10
FT /note="L -> W (in strain: JR-142 and JR-460)"
FT VARIANT 18
FT /note="S -> F (in strain: GB-8 and JR-50)"
FT VARIANT 23
FT /note="A -> V (in strain: GB-115, GB-336, JR-38, JR-106,
FT JR-198 and JR-382)"
FT VARIANT 24
FT /note="D -> Y (in strain: GB-336)"
FT VARIANT 26
FT /note="L -> M (in strain: GB-4, JR-126 and JR-460)"
FT VARIANT 83
FT /note="L -> T (in strain: JR-198 and JR-460)"
FT VARIANT 93
FT /note="G -> E (in strain: JR-50)"
FT VARIANT 110
FT /note="K -> R (in strain: GB-115, JR-50, JR-106, JR-126,
FT JR-142, JR-198, JR-341 and JR-382)"
FT VARIANT 154..155
FT /note="IS -> RG (in strain: JR-32)"
FT VARIANT 155
FT /note="S -> T (in strain: JR-126)"
FT VARIANT 170
FT /note="D -> N (in strain: GB-4, GB-139 and JR-38)"
FT VARIANT 171
FT /note="L -> F (in strain: JR-106)"
FT VARIANT 193
FT /note="S -> R (in strain: JR-126)"
FT VARIANT 211
FT /note="G -> A (in strain: JR-126)"
FT VARIANT 225
FT /note="L -> V (in strain: GB-4, GB-115 and JR-142)"
FT VARIANT 248
FT /note="G -> L (in strain: JR-38 and JR-382)"
FT VARIANT 285
FT /note="R -> G (in strain: GB-115)"
FT VARIANT 291
FT /note="A -> S (in strain: GB-4)"
FT VARIANT 313
FT /note="S -> T (in strain: GB-329, GB-336, JR-32 and JR-
FT 460)"
FT VARIANT 317
FT /note="V -> I (in strain: GB-329, GB-336, JR-32 and JR-
FT 460)"
FT VARIANT 326
FT /note="A -> S (in strain: GB-329 and JR-32)"
FT VARIANT 327
FT /note="Q -> K (in strain: GB-336, JR-126 and JR-460)"
FT VARIANT 334
FT /note="Y -> C (in strain: JR-106)"
FT VARIANT 386
FT /note="D -> Y (in strain: JR-341)"
FT VARIANT 444
FT /note="D -> E (in strain: GB-336)"
FT VARIANT 474
FT /note="I -> M (in strain: GB-336, JR-32, JR-50, JR-106 and
FT JR-382)"
FT VARIANT 488
FT /note="E -> K (in strain: GB-329)"
FT VARIANT 491
FT /note="I -> V (in strain: GB-8)"
FT VARIANT 514
FT /note="T -> A (in strain: GB-336, JR-32, JR-50, JR-106 and
FT JR-126)"
FT VARIANT 523
FT /note="S -> R (in strain: GB-139, GB-329, JR-142 and JR-
FT 460)"
FT VARIANT 532
FT /note="T -> S (in strain: JR-341)"
FT VARIANT 533
FT /note="R -> Q (in strain: JR-50)"
FT CONFLICT 92
FT /note="Q -> R (in Ref. 1; AAA28515)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 545 AA; 60652 MW; 3C329272BDBE5CCE CRC64;
MYCAKLILLL GCFWISSSAS DPADPLLVDL PNGKLRGRDN GNYYSYESLP YAEPPVGDLR
FEAPQPYKQQ WTDTFDATQP PVLCMQWDQF IQGDDKLAGN EDCLTVSVYK PKNSSRNSFP
VVAQIHGGAF MFGGASQNGH ENFMREGNLI LVKISYRLGP LGFVSTGDAD LSGNFGLKDQ
RLALLWIKQN IASFGGEPEN ILVIGHSAGG GSVHLQVLRE DFSKLAKAAI SFSGNALDPW
VVQQGGRGRA FELGRIVGCG QASDSVTLKK CLKSKPASEI VSAVRNFLVF AYVPFTPFGP
VVESPDAPEA FISQHPVDII KSGKFAQVPW AVTYTTEDGG YNAALLLEKQ ASSGRELIVD
LNDRWFDWAP YLLFYRDSMT TIKDMDDYSR KLRQEYLGDR RFSVESYWDL QRLFTDVLFK
NSTEISLDLH RKHGKSPVYA FVYDNPANTG IGQGLAKRTD INFGTVHGDD YFLIFENIVR
EPQLRSDEEI ISRNFLKMLN DFVLSENGTL AFGTCVFQDN VGSSKLQLLS ITRNGCENLE
LESFP
