ID   EST5C_DROPS             Reviewed;         545 AA.
AC   P25725; O16180; O16181; O16182; O16183; O16184; O16185; O16186;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Esterase-5C;
DE            Short=Est-5C;
DE            EC=3.1.1.1;
DE   AltName: Full=Carboxylic-ester hydrolase 5C;
DE            Short=Carboxylesterase-5C;
DE   Flags: Precursor;
GN   Name=Est-5C; Synonyms=Est5C; ORFNames=GA19955;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2178209; DOI=10.1093/oxfordjournals.molbev.a040624;
RA   Brady J.P., Richmond R.C., Oakeshott J.G.;
RT   "Cloning of the esterase-5 locus from Drosophila pseudoobscura and
RT   comparison with its homologue in D. melanogaster.";
RL   Mol. Biol. Evol. 7:525-546(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=GB115E, GB139E, GB336E, GB4E, GB8E, JR198E, JR341E, and JR50E;
RX   PubMed=9475741; DOI=10.1093/genetics/148.1.305;
RA   King L.M.;
RT   "The role of gene conversion in determining sequence variation and
RT   divergence in the Est-5 gene family in Drosophila pseudoobscura.";
RL   Genetics 148:305-315(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25 / Tucson 14011-0121.94;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA   Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA   Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA   Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA   Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA   Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA   Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT   gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC         Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10039};
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC       {ECO:0000305}.
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DR   EMBL; M55907; AAA28514.1; -; Genomic_DNA.
DR   EMBL; AF016143; AAB70233.1; -; Genomic_DNA.
DR   EMBL; AF016144; AAB70234.1; -; Genomic_DNA.
DR   EMBL; AF016145; AAB70235.1; -; Genomic_DNA.
DR   EMBL; AF016146; AAB70236.1; -; Genomic_DNA.
DR   EMBL; AF016147; AAB70237.1; -; Genomic_DNA.
DR   EMBL; AF016148; AAB70238.1; -; Genomic_DNA.
DR   EMBL; AF016149; AAB70239.1; -; Genomic_DNA.
DR   EMBL; AF016150; AAB70240.1; -; Genomic_DNA.
DR   EMBL; CH379069; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P25725; -.
DR   SMR; P25725; -.
DR   STRING; 7237.FBpp0274977; -.
DR   ESTHER; drops-est5c; Carb_B_Arthropoda.
DR   MEROPS; S09.947; -.
DR   eggNOG; KOG1516; Eukaryota.
DR   InParanoid; P25725; -.
DR   Proteomes; UP000001819; Genome assembly.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002018; CarbesteraseB.
DR   InterPro; IPR019826; Carboxylesterase_B_AS.
DR   InterPro; IPR019819; Carboxylesterase_B_CS.
DR   Pfam; PF00135; COesterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR   PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Glycoprotein; Hydrolase; Reference proteome; Secreted;
KW   Serine esterase; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..545
FT                   /note="Esterase-5C"
FT                   /id="PRO_0000008560"
FT   ACT_SITE        207
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT   ACT_SITE        467
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        113
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        421
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        507
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        84..103
FT                   /evidence="ECO:0000250"
FT   DISULFID        259..271
FT                   /evidence="ECO:0000250"
FT   DISULFID        515..536
FT                   /evidence="ECO:0000255"
FT   VARIANT         6
FT                   /note="L -> R (in strain: GB8E)"
FT   VARIANT         11
FT                   /note="S -> R (in strain: GB4E)"
FT   VARIANT         42
FT                   /note="F -> N (in strain: GB8E and GB336E)"
FT   VARIANT         58
FT                   /note="E -> D (in strain: JR50E and GB139E)"
FT   VARIANT         98
FT                   /note="V -> A (in strain: JR50E, GB8E and GB115E)"
FT   VARIANT         130
FT                   /note="F -> S (in strain: JR341E)"
FT   VARIANT         170
FT                   /note="D -> G (in strain: GB4E, JR50E, GB115E, GB139E,
FT                   GB336E and JR341E)"
FT   VARIANT         266
FT                   /note="M -> T (in strain: GB4E, JR50E, GB115E, GB139E,
FT                   GB336E and JR341E)"
FT   VARIANT         291
FT                   /note="A -> G (in strain: JR50E)"
FT   VARIANT         307
FT                   /note="A -> T (in strain: GB115E)"
FT   VARIANT         461
FT                   /note="V -> I (in strain: GB336E)"
FT   VARIANT         537
FT                   /note="E -> V (in strain: JR50E and JR341E)"
SQ   SEQUENCE   545 AA;  61021 MW;  60535F4DFA639D6A CRC64;
     MLAARLIILL SFYWLSASAI DPADPLFVDL PHGKIRGRDN GFYYSYESLP YAEPPVGELR
     FEAPQPYKQQ WTDTFDATQP PVTCMQWNQF IFGDNKLVGV EDCLTVSIYK PKNTSQSSFP
     VVAHMHGGAF MFGEARQNGH ENMMREGKLI LVKISYRLGP LGFASTGDAD LSGNFGLKDQ
     RLALLWIKQN IASFGGEPEN IIVVGHSAGG ASVHLQMLRE DFAQVAKAGI SFGGNAMDPW
     VIHQSARGRT FELGRIVGCG QASDSMELKN CLKSKPAGEI VSAVHSFLVF AYVPFAPFGP
     VVESPDAPEA FISQHPVDII KSGKFAQVPW AVTYNTEDGG YNAAVLLEKQ ASSGRELIFD
     LNDHWFDWAP YLLFYRDSMT TIKDMDDYSR KLRQEYLGDR RFSVESYWDL QRLFTDILYK
     NATELALDLY RKHGKSPVYA FVYDNPANTG IGQFFAKRTD VHFGTVHGDE YFLIFENLAR
     GPEMRSDEEI ISRNFLNMIN DFVVSGNGTM TFGNCVLQDN VGSNKFQLLS ITKNGCENLQ
     LESFP