EST6_DROMA
ID EST6_DROMA Reviewed; 542 AA.
AC P47982; Q670K3; Q9GNC2; Q9GQA4; Q9GQA5; Q9GQA6;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Esterase 6;
DE Short=Est-6;
DE EC=3.1.1.1;
DE AltName: Full=Carboxylic-ester hydrolase 6;
DE Short=Carboxylesterase-6;
DE Flags: Precursor;
GN Name=Est-6; Synonyms=est6;
OS Drosophila mauritiana (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7226;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8375665; DOI=10.1007/bf02424448;
RA Karotam J., Delves A.C., Oakeshott J.G.;
RT "Conservation and change in structural and 5' flanking sequences of
RT esterase 6 in sibling Drosophila species.";
RL Genetica 88:11-28(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Balakirev E.S., Chechetkin V.R., Lobzin V.V., Ayala F.J.;
RT "Entropy and GC content in the b-esterase gene cluster of Drosophila
RT melanogaster subgroup.";
RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-532.
RC STRAIN=105, 152, 197, 207, Lig72, and Lig74;
RX PubMed=11102384; DOI=10.1093/genetics/156.4.1913;
RA Kliman R.M., Andolfatto P., Coyne J.A., Depaulis F., Kreitman M.,
RA Berry A.J., McCarter J., Wakeley J., Hey J.;
RT "The population genetics of the origin and divergence of the Drosophila
RT simulans complex species.";
RL Genetics 156:1913-1931(2000).
CC -!- FUNCTION: Transferred from the ejaculatory bulbs of males to the female
CC genitals upon copulation, plays an important role in the reproductive
CC biology.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10039};
CC -!- SUBUNIT: Monomer.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC {ECO:0000305}.
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DR EMBL; L10671; AAA03158.1; -; Genomic_DNA.
DR EMBL; AY695921; AAU05619.1; -; Genomic_DNA.
DR EMBL; AF284482; AAG28715.1; -; Genomic_DNA.
DR EMBL; AF284483; AAG28716.1; -; Genomic_DNA.
DR EMBL; AF284484; AAG28717.1; -; Genomic_DNA.
DR EMBL; AF284485; AAG28718.1; -; Genomic_DNA.
DR EMBL; AF284486; AAG28719.1; -; Genomic_DNA.
DR EMBL; AF284487; AAG28720.1; -; Genomic_DNA.
DR AlphaFoldDB; P47982; -.
DR SMR; P47982; -.
DR ESTHER; droma-este6; Carb_B_Arthropoda.
DR MEROPS; S09.947; -.
DR FlyBase; FBgn0012501; Dmau\Est-6.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002018; CarbesteraseB.
DR InterPro; IPR019826; Carboxylesterase_B_AS.
DR InterPro; IPR019819; Carboxylesterase_B_CS.
DR Pfam; PF00135; COesterase; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Glycoprotein; Hydrolase; Secreted; Serine esterase; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..542
FT /note="Esterase 6"
FT /id="PRO_0000008563"
FT ACT_SITE 207
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT ACT_SITE 464
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT CARBOHYD 40
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 418
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 454
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 84..103
FT /evidence="ECO:0000250"
FT DISULFID 259..271
FT /evidence="ECO:0000250"
FT DISULFID 512..533
FT /evidence="ECO:0000255"
FT VARIANT 56
FT /note="I -> T (in strain: 197)"
FT VARIANT 199..201
FT /note="ENI -> QNV (in strain: 197)"
FT VARIANT 302
FT /note="L -> M (in strain: Lig72)"
FT VARIANT 355
FT /note="A -> S (in strain: Lig74)"
FT VARIANT 391
FT /note="E -> Q (in strain: Lig74)"
FT VARIANT 394
FT /note="L -> I (in strain: Lig74)"
FT VARIANT 494
FT /note="N -> K (in strain: 197)"
FT VARIANT 496
FT /note="L -> M (in strain: Lig72)"
SQ SEQUENCE 542 AA; 60890 MW; 3E7ED31DA51E12F1 CRC64;
MNYVGLIIVL SCLWLGSNAS DPDDPLLVQL PQGKLRGRDN GSYYSYESIP YAEPPIGDLR
FEAPEPYKQK WSDIFEATKT PVACLQWDQF TPGANKLVGE EDCLTVSIYK PKNSKRSSFP
VVAHIHGGAF MFGAAWQNGH ENVMREGKFI LVKISYRLGP LGFASTGDRD LPGNYGLKDQ
RLALKWIKQN IASFGGEPEN ILLIGHSAGG ASVHLQMLRE DFGQLAKAAF SFSGNALDPW
VVQKGARGRA FELGRNVGCE SSEDSASLKK CLKSKPASEL VTAVRKFLIF SYVPFAPFSP
VLEPSDAPDA FLTQDPREVI KSGKFGQVPW AVSYVTEDGG YNAALLLKER KSGIAIDDLN
DRWLELAPYF LFYRDTKTKK DMDDYSRKIK EDYLGNQKFD IESYSELQRL FTDILFKNST
QESLDLHRKY GKSPAYAYVY DNPAEKGIAQ VLANRTDYDF GTVHGDDYFL IFENFVREVE
MRPDEEIISR NFINMLADFA SSDNGVLKYG ECAFKNNVGS EKFQLLAIYI DGCQNRQHVE
FP
