ESTA_PSEPU
ID ESTA_PSEPU Reviewed; 646 AA.
AC Q6B6R8;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Esterase EstA;
DE EC=3.1.1.1;
DE Flags: Precursor;
GN Name=estA;
OS Pseudomonas putida (Arthrobacter siderocapsulatus).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=303;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC STRAIN=3SK;
RX PubMed=15574889; DOI=10.1128/aem.70.12.6968-6976.2004;
RA Yang T.H., Pan J.G., Seo Y.S., Rhee J.S.;
RT "Use of Pseudomonas putida EstA as an anchoring motif for display of a
RT periplasmic enzyme on the surface of Escherichia coli.";
RL Appl. Environ. Microbiol. 70:6968-6976(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000269|PubMed:15574889}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15574889}.
CC -!- DOMAIN: Contains a C-terminal autotransporter domain that integrates
CC into the outer membrane and enables the translocation of the catalytic
CC N-terminal domain to the bacterial cell surface.
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
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DR EMBL; AY676875; AAT77203.1; -; Genomic_DNA.
DR AlphaFoldDB; Q6B6R8; -.
DR SMR; Q6B6R8; -.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016298; F:lipase activity; IEA:InterPro.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR Gene3D; 2.40.128.130; -; 1.
DR Gene3D; 3.40.50.1110; -; 1.
DR InterPro; IPR005546; Autotransporte_beta.
DR InterPro; IPR036709; Autotransporte_beta_dom_sf.
DR InterPro; IPR001087; GDSL.
DR InterPro; IPR017186; Lipase_autotranspt_EstA.
DR InterPro; IPR008265; Lipase_GDSL_AS.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR Pfam; PF03797; Autotransporter; 1.
DR Pfam; PF00657; Lipase_GDSL; 1.
DR PIRSF; PIRSF037375; Autotrns_EstA; 1.
DR SMART; SM00869; Autotransporter; 1.
DR SUPFAM; SSF103515; SSF103515; 1.
DR PROSITE; PS51208; AUTOTRANSPORTER; 1.
DR PROSITE; PS01098; LIPASE_GDSL_SER; 1.
PE 3: Inferred from homology;
KW Cell outer membrane; Hydrolase; Membrane; Signal; Transmembrane;
KW Transmembrane beta strand.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..646
FT /note="Esterase EstA"
FT /id="PRO_0000017846"
FT TOPO_DOM 25..397
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..408
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 409..410
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..421
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 422..437
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 438..447
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 448..451
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 452..461
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 462..488
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 489..500
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 501..507
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 508..518
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 519..547
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 548..558
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 559..561
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 562..571
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 572..605
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 606..615
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 616..618
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 619..628
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT TOPO_DOM 629..636
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 637..646
FT /note="Beta stranded"
FT /evidence="ECO:0000255"
FT DOMAIN 366..646
FT /note="Autotransporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00556"
FT ACT_SITE 38
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 310
FT /evidence="ECO:0000250"
FT ACT_SITE 313
FT /evidence="ECO:0000250"
SQ SEQUENCE 646 AA; 69609 MW; AFF3CC4FFE25A191 CRC64;
MIRMALKPLV AACLLASLST APQAAPSPYS TLVVFGDSLS DAGQFPDPAG PAGSTSRFTN
RVGPTYQNGS GEIFGPTAPM LLGNQLGIAP GDLAASTSPV NAQQGIADGN NWAVGGYRTD
QIYDSITAAN GSLIERDNTL LRSRDGYLVD RARQGLGADP NALYYITGGG NDFLQGRILN
DVQAQQAAGR LVDSVQALQQ AGARYIVVWL LPDLGLTPAT FGGPLQPFAS QLSGTFNAEL
TAQLSQAGAN VIPLNIPLLL KEGMANPASF GLAADQNLIG TCFSGNGCTM NPTYGINGST
PDPSKLLFND SVHPTITGQR LIADYTYSLL SAPWELTLLP EMAHGTLRAY QDELRSQWQA
DWENWQNVGQ WRGFVGGGGQ RLDFDSQDSA ASGDGNGYNL TLGGSYRIDE AWRAGVAAGF
YRQKLEAGAK DSDYRMNSYM ASAFVQYQEN RWWADAALTG GYLDYDDLKR KFALGGGERS
EKGDTNGHLW AFSARLGYDI AQQADSPWHL SPFVSADYAR VEVDGYSEKG ASATALDYDD
QKRSSKRLGA GLQGKYAFGS DTQLFAEYAH EREYEDDTQD LTMSLNSLPG NRFTLEGYTP
QDHLNRVSLG FSQKLAPELS LRGGYNWRKG EDDTQQSVSL ALSLDF
