ID   ESTD_BOVIN              Reviewed;         282 AA.
AC   Q08E20;
DT   01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=S-formylglutathione hydrolase;
DE            Short=FGH;
DE            EC=3.1.2.12;
DE   AltName: Full=Esterase D;
GN   Name=ESD;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Thalamus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Serine hydrolase involved in the detoxification of
CC       formaldehyde. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + S-formylglutathione = formate + glutathione + H(+);
CC         Xref=Rhea:RHEA:14961, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:57688, ChEBI:CHEBI:57925; EC=3.1.2.12;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Cytoplasmic vesicle {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the esterase D family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC123461; AAI23462.1; -; mRNA.
DR   RefSeq; NP_001069533.1; NM_001076065.1.
DR   RefSeq; XP_005213718.1; XM_005213661.2.
DR   RefSeq; XP_005213719.1; XM_005213662.3.
DR   AlphaFoldDB; Q08E20; -.
DR   SMR; Q08E20; -.
DR   STRING; 9913.ENSBTAP00000004431; -.
DR   ESTHER; bovin-estd; A85-EsteraseD-FGH.
DR   PaxDb; Q08E20; -.
DR   PeptideAtlas; Q08E20; -.
DR   PRIDE; Q08E20; -.
DR   Ensembl; ENSBTAT00000004431; ENSBTAP00000004431; ENSBTAG00000003415.
DR   GeneID; 535653; -.
DR   KEGG; bta:535653; -.
DR   CTD; 2098; -.
DR   VEuPathDB; HostDB:ENSBTAG00000003415; -.
DR   VGNC; VGNC:28600; ESD.
DR   eggNOG; KOG3101; Eukaryota.
DR   GeneTree; ENSGT00390000011864; -.
DR   HOGENOM; CLU_056472_0_0_1; -.
DR   InParanoid; Q08E20; -.
DR   OrthoDB; 942551at2759; -.
DR   TreeFam; TF300793; -.
DR   Proteomes; UP000009136; Chromosome 12.
DR   Bgee; ENSBTAG00000003415; Expressed in esophagus and 106 other tissues.
DR   ExpressionAtlas; Q08E20; baseline and differential.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0018738; F:S-formylglutathione hydrolase activity; IBA:GO_Central.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:InterPro.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000801; Esterase-like.
DR   InterPro; IPR014186; S-formylglutathione_hydrol.
DR   PANTHER; PTHR10061; PTHR10061; 1.
DR   Pfam; PF00756; Esterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   TIGRFAMs; TIGR02821; fghA_ester_D; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Cytoplasm; Cytoplasmic vesicle; Hydrolase; Reference proteome;
KW   Serine esterase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q9R0P3"
FT   CHAIN           2..282
FT                   /note="S-formylglutathione hydrolase"
FT                   /id="PRO_0000341965"
FT   ACT_SITE        149
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        226
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        260
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9R0P3"
FT   MOD_RES         4
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9R0P3"
SQ   SEQUENCE   282 AA;  31548 MW;  42CEC96886B147F4 CRC64;
     MALKQVSSSK CFGGLQKVFE HDSVELKCKM KFAVYLPPKA ETGKCPVLYW LSGLTCTEQN
     FISKSGYHQA ASEHGLVVIA PDTSPRGCNI KGEEDSWDFG TGAGFYVDAT EDLWKTNYRM
     YSYVTKELPQ LVNDNFPVDP QRMSVFGHSM GGHGALICAL KNPGKYKSVS AFAPLCNPVL
     CRWGKKAFTG YLGTDQSKWE AYDATYLVKS YPGPQLDILI DQGKEDEFLS DGQLLPDNFI
     AACTEKKIPV VFRLQEGYDH SYYFIATFIA DHIRHHAKYL NA