AGRG3_MOUSE
ID AGRG3_MOUSE Reviewed; 542 AA.
AC Q8R0T6; Q3U2Q3;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Adhesion G protein-coupled receptor G3;
DE AltName: Full=G-protein coupled receptor 97;
DE Flags: Precursor;
GN Name=Adgrg3; Synonyms=Gpr97;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=24113187; DOI=10.1038/cddis.2013.346;
RA Wang J.J., Zhang L.L., Zhang H.X., Shen C.L., Lu S.Y., Kuang Y., Wan Y.H.,
RA Wang W.G., Yan H.M., Dang S.Y., Fei J., Jin X.L., Wang Z.G.;
RT "Gpr97 is essential for the follicular versus marginal zone B-lymphocyte
RT fate decision.";
RL Cell Differ. 4:E853-E853(2013).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=24178298; DOI=10.1074/jbc.m113.512954;
RA Valtcheva N., Primorac A., Jurisic G., Hollmen M., Detmar M.;
RT "The orphan adhesion G protein-coupled receptor GPR97 regulates migration
RT of lymphatic endothelial cells via the small GTPases RhoA and Cdc42.";
RL J. Biol. Chem. 288:35736-35748(2013).
CC -!- FUNCTION: Orphan receptor that regulates migration of lymphatic
CC endothelial cells via the small GTPases RhoA and CDC42 (By similarity).
CC Regulates B-cell development (PubMed:24113187). Seems to signal through
CC G-alpha(q)-proteins (By similarity). {ECO:0000250|UniProtKB:Q86Y34,
CC ECO:0000269|PubMed:24113187}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q86Y34}; Multi-
CC pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in intestinal lymphatic
CC endothelium. {ECO:0000269|PubMed:24178298}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Mutant mice show altered
CC follicular-versus-marginal zone B-lymphocyte fate decision in the
CC spleen and decreased numbers of B220+ lymphocytes in the bone marrow.
CC {ECO:0000269|PubMed:24113187}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 2 family.
CC Adhesion G-protein coupled receptor (ADGR) subfamily. {ECO:0000305}.
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DR EMBL; AK155164; BAE33087.1; -; mRNA.
DR EMBL; BC026426; AAH26426.1; -; mRNA.
DR EMBL; BC064726; AAH64726.1; -; mRNA.
DR CCDS; CCDS22554.1; -.
DR RefSeq; NP_766624.3; NM_173036.3.
DR AlphaFoldDB; Q8R0T6; -.
DR SMR; Q8R0T6; -.
DR IntAct; Q8R0T6; 1.
DR STRING; 10090.ENSMUSP00000051079; -.
DR MEROPS; P02.024; -.
DR GlyGen; Q8R0T6; 4 sites.
DR iPTMnet; Q8R0T6; -.
DR PhosphoSitePlus; Q8R0T6; -.
DR jPOST; Q8R0T6; -.
DR MaxQB; Q8R0T6; -.
DR PaxDb; Q8R0T6; -.
DR PRIDE; Q8R0T6; -.
DR ProteomicsDB; 285773; -.
DR Antibodypedia; 15147; 163 antibodies from 27 providers.
DR DNASU; 54672; -.
DR Ensembl; ENSMUST00000051259; ENSMUSP00000051079; ENSMUSG00000060470.
DR GeneID; 54672; -.
DR KEGG; mmu:54672; -.
DR UCSC; uc009mxp.2; mouse.
DR CTD; 222487; -.
DR MGI; MGI:1859670; Adgrg3.
DR VEuPathDB; HostDB:ENSMUSG00000060470; -.
DR eggNOG; KOG4193; Eukaryota.
DR GeneTree; ENSGT00940000154285; -.
DR HOGENOM; CLU_002753_3_9_1; -.
DR InParanoid; Q8R0T6; -.
DR OMA; TLFKGPQ; -.
DR OrthoDB; 501343at2759; -.
DR PhylomeDB; Q8R0T6; -.
DR TreeFam; TF321769; -.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 54672; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Adgrg3; mouse.
DR PRO; PR:Q8R0T6; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8R0T6; protein.
DR Bgee; ENSMUSG00000060470; Expressed in granulocyte and 79 other tissues.
DR ExpressionAtlas; Q8R0T6; baseline and differential.
DR Genevisible; Q8R0T6; MM.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:MGI.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0030183; P:B cell differentiation; IMP:UniProtKB.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0032792; P:negative regulation of CREB transcription factor activity; IMP:UniProtKB.
DR GO; GO:1901223; P:negative regulation of NIK/NF-kappaB signaling; IMP:UniProtKB.
DR GO; GO:0030334; P:regulation of cell migration; ISS:UniProtKB.
DR Gene3D; 2.60.220.50; -; 1.
DR InterPro; IPR046338; GAIN_dom_sf.
DR InterPro; IPR017981; GPCR_2-like.
DR InterPro; IPR000832; GPCR_2_secretin-like.
DR InterPro; IPR003910; GPR1/GPR3/GPR5.
DR InterPro; IPR000203; GPS.
DR Pfam; PF00002; 7tm_2; 1.
DR Pfam; PF01825; GPS; 1.
DR PRINTS; PR00249; GPCRSECRETIN.
DR PRINTS; PR01422; GPR56ORPHANR.
DR SMART; SM00303; GPS; 1.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
DR PROSITE; PS50221; GPS; 1.
PE 2: Evidence at transcript level;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Signal; Transducer; Transmembrane; Transmembrane helix.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..542
FT /note="Adhesion G protein-coupled receptor G3"
FT /id="PRO_0000012890"
FT TOPO_DOM 19..267
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 268..288
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 289..302
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 303..323
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 324..342
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 343..363
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 364..372
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 373..393
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 394..426
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 427..447
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 448..467
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 468..488
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 489..494
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 495..515
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 516..542
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT DOMAIN 210..256
FT /note="GPS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00098"
FT CARBOHYD 44
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 96
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 142
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 408
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 295
FT /note="F -> L (in Ref. 2; AAH64726)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 542 AA; 60072 MW; E37A23B108BA1CB3 CRC64;
MATARSLGLL FFLLLTSDEE TTEEPRNVCR RLQEGHEYDT FDLNDTAQCF TKCGQSEHSP
CDVGNLQRYW LNYESYLLEN SMETVDMPFV KALIQNISTD VSEDLLYSLM LSQIPRQVMQ
GEDEPADGVR LPKSLFGALP GNRSAVRLAI TVLDIGAGNV FKGPKLLEDK GSSVLNNRMV
GLSVGQMHAT GLSEPVEITF SHERQPPNVI LTCVFWDMAK GDWDSHGCST VPGDGRTVCR
CDHLTFFALL LRPILDLATA QTLTRISQAG SAVSMIFLAF TMVLYVAFRF SLQRFKSEDA
PKIHMALSIS LFLLNLTFLI NVGSSSQGPP ASCWVRAAIF HYFLLCVFTW MGLEAFHLYL
LAIRVFNTYF GHYFLKLSLL AWGLPVLVVI GAGSSNSYGV YTIRDQENRT SLELCWFQKE
PALYATVHGY FLVTFLFGAV VLALVAWKIF TLPSVTAGKG QGPTWKSVLT VLGLSSLVGM
TWGLAVLTPL GLSTIYVFTL LNSLQGLFIF CWFIILYFPT QSTTASSSGT ARLDQAHSVS
QE
