ID   ESTJ_HELVI              Reviewed;         564 AA.
AC   P12992;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Juvenile hormone esterase;
DE            Short=JH esterase;
DE            EC=3.1.1.59;
DE   Flags: Precursor;
OS   Heliothis virescens (Tobacco budworm moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Noctuoidea;
OC   Noctuidae; Heliothinae; Heliothis.
OX   NCBI_TaxID=7102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 20-54.
RX   PubMed=2745451; DOI=10.1016/s0021-9258(18)63875-x;
RA   Hanzlik T.N., Yehia A.I.A.-A., Harshman L.G., Hammock B.D.;
RT   "Isolation and sequencing of cDNA clones coding for juvenile hormone
RT   esterase from Heliothis virescens. Evidence for a catalytic mechanism for
RT   the serine carboxylesterases different from that of the serine proteases.";
RL   J. Biol. Chem. 264:12419-12425(1989).
RN   [2]
RP   SEQUENCE REVISION.
RA   Hanzlik T.N.;
RL   Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: JH esterase plays a crucial role in the decrease of JH
CC       activity in lepidopteran insects, by hydrolyzing the methyl ester of
CC       JH. It is also involved in the transport of JH.
CC       {ECO:0000250|UniProtKB:P19985}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + juvenile hormone I = H(+) + juvenile hormone I
CC         carboxylate + methanol; Xref=Rhea:RHEA:46916, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:83641,
CC         ChEBI:CHEBI:87109; EC=3.1.1.59;
CC         Evidence={ECO:0000250|UniProtKB:P19985};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + juvenile hormone III = H(+) + juvenile hormone III
CC         carboxylate + methanol; Xref=Rhea:RHEA:46912, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17790, ChEBI:CHEBI:27493,
CC         ChEBI:CHEBI:83274; EC=3.1.1.59;
CC         Evidence={ECO:0000250|UniProtKB:P19985};
CC   -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC       {ECO:0000305}.
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DR   EMBL; J04955; AAB88629.1; -; mRNA.
DR   PIR; A34325; A34325.
DR   AlphaFoldDB; P12992; -.
DR   SMR; P12992; -.
DR   ESTHER; helvi-jhest; Juvenile_hormone_esterase.
DR   BRENDA; 3.1.1.59; 2613.
DR   GO; GO:0004453; F:juvenile-hormone esterase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002018; CarbesteraseB.
DR   InterPro; IPR019826; Carboxylesterase_B_AS.
DR   Pfam; PF00135; COesterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein; Hydrolase;
KW   Serine esterase; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|PubMed:2745451"
FT   CHAIN           20..564
FT                   /note="Juvenile hormone esterase"
FT                   /id="PRO_0000008629"
FT   ACT_SITE        220
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT   ACT_SITE        351
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        465
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        81
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        180
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        402
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        515
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        89..109
FT                   /evidence="ECO:0000250"
FT   VARIANT         29
FT                   /note="V -> L"
FT   VARIANT         52
FT                   /note="F -> P"
SQ   SEQUENCE   564 AA;  62615 MW;  D140E5DD91914E8D CRC64;
     MTSHVLALAF LLHACTALAW QETNSRSVVA HLDSGIIRGV PRSADGIKFA SFLGVPYAKQ
     PVGELRFKEL EPLEPWDNIL NATNEGPICF QTDVLYGRLM AASEMSEACI YANIHVPWQS
     LPRVRGTTPL RPILVFIHGG GFAFGSGHED LHGPEYLVTK NVIVITFNYR LNVFGFLSMN
     TTKIPGNAGL RDQVTLLRWV QRNAKNFGGD PSDITIAGQS AGASAAHLLT LSKATEGLFK
     RAILMSGTGM SYFFTTSPLF AAYISKQLLQ ILGINETDPE EIHRQLIDLP AEKLNEANAV
     LIEQIGLTTF LPIVESPLPG VTTIIDDDPE ILIAEGRGKN VPLLIGFTSS ECETFRNRLL
     NFDLVKKIQD NPTIIIPPKL LFMTPPELLM ELAKTIERKY YNGTISIDNF VKSCSDGFYE
     YPALKLAQKR AETGGAPLYL YRFAYEGQNS IIKKVMGLNH EGVGHIEDLT YVFKVNSMSE
     ALHASPSEND VKMKNLMTGY FLNFIKCSQP TCEDNNSLEV WPANNGMQYE DIVSPTIIRS
     KEFASRQQDI IEFFDSFTSR SPLE