ESTP_DROME
ID ESTP_DROME Reviewed; 544 AA.
AC P18167; Q32KD6; Q9VTV0;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2005, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Esterase P;
DE Short=Est-P;
DE EC=3.1.1.1;
DE AltName: Full=Carboxylic-ester hydrolase P;
DE Short=Carboxylesterase-P;
DE Flags: Precursor;
GN Name=Est-P; Synonyms=EstP; ORFNames=CG17148;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Canton-S;
RX PubMed=2105433; DOI=10.1093/oxfordjournals.molbev.a040582;
RA Collet C., Nielsen K.M., Russell R.J., Karl M., Oakeshott J.G.,
RA Richmond R.C.;
RT "Molecular analysis of duplicated esterase genes in Drosophila
RT melanogaster.";
RL Mol. Biol. Evol. 7:9-28(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10039};
CC -!- SUBUNIT: Monomer.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- DEVELOPMENTAL STAGE: Mainly in late larvae.
CC -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC {ECO:0000305}.
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DR EMBL; M33780; AAA28520.1; -; Genomic_DNA.
DR EMBL; AE014296; AAF49945.1; -; Genomic_DNA.
DR EMBL; BT023943; ABB36447.1; -; mRNA.
DR PIR; B34089; B34089.
DR RefSeq; NP_788501.1; NM_176323.2.
DR AlphaFoldDB; P18167; -.
DR SMR; P18167; -.
DR IntAct; P18167; 1.
DR STRING; 7227.FBpp0075736; -.
DR ESTHER; drome-est6p; Carb_B_Arthropoda.
DR GlyGen; P18167; 4 sites.
DR PaxDb; P18167; -.
DR DNASU; 39393; -.
DR EnsemblMetazoa; FBtr0076004; FBpp0075736; FBgn0000594.
DR GeneID; 39393; -.
DR KEGG; dme:Dmel_CG17148; -.
DR CTD; 39393; -.
DR FlyBase; FBgn0000594; Est-P.
DR VEuPathDB; VectorBase:FBgn0000594; -.
DR eggNOG; KOG1516; Eukaryota.
DR GeneTree; ENSGT00940000173305; -.
DR HOGENOM; CLU_006586_13_2_1; -.
DR InParanoid; P18167; -.
DR OMA; WIDLLND; -.
DR OrthoDB; 754103at2759; -.
DR PhylomeDB; P18167; -.
DR Reactome; R-DME-112311; Neurotransmitter clearance.
DR Reactome; R-DME-1483191; Synthesis of PC.
DR Reactome; R-DME-2022377; Metabolism of Angiotensinogen to Angiotensins.
DR Reactome; R-DME-211945; Phase I - Functionalization of compounds.
DR Reactome; R-DME-8964038; LDL clearance.
DR Reactome; R-DME-9749641; Aspirin ADME.
DR BioGRID-ORCS; 39393; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 39393; -.
DR PRO; PR:P18167; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0000594; Expressed in saliva-secreting gland and 8 other tissues.
DR Genevisible; P18167; DM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IDA:FlyBase.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR GO; GO:0017171; F:serine hydrolase activity; HDA:FlyBase.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002018; CarbesteraseB.
DR InterPro; IPR019826; Carboxylesterase_B_AS.
DR InterPro; IPR019819; Carboxylesterase_B_CS.
DR Pfam; PF00135; COesterase; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Hydrolase; Reference proteome; Secreted;
KW Serine esterase; Signal.
FT SIGNAL 1..19
FT CHAIN 20..544
FT /note="Esterase P"
FT /id="PRO_0000008562"
FT ACT_SITE 206
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10039"
FT ACT_SITE 466
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT CARBOHYD 75
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 114
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 262
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 456
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 83..102
FT /evidence="ECO:0000250"
FT DISULFID 258..270
FT /evidence="ECO:0000250"
FT DISULFID 514..535
FT /evidence="ECO:0000255"
FT CONFLICT 50
FT /note="N -> Y (in Ref. 1; AAA28520)"
FT /evidence="ECO:0000305"
FT CONFLICT 155..156
FT /note="FG -> YR (in Ref. 1; AAA28520)"
FT /evidence="ECO:0000305"
FT CONFLICT 481
FT /note="T -> I (in Ref. 1; AAA28520)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 544 AA; 61054 MW; 835FE81D94B46623 CRC64;
MSIFKRLLCL TLLWIAALES EADPLIVEIT NGKIRGKDNG LYYSYESIPN AEHPTGALRF
EAPQPYSHHW TDVFNATQSP VECMQWNQFI NENNKLMGDE DCLTVSIYKP KKPNRSSFPV
VVLLHGGAFM FGSGSIYGHD SIMREGTLLV VKISFGLGPL GFASTGDRHL PGNYGLKDQR
LALQWIKKNI AHFGGMPDNI VLIGHSAGGA SAHLQLLHED FKHLAKGAIS VSGNALDPWV
IQQGGRRRAF ELGRIVGCGH TNVSAELKDC LKSKPASDIV SAVRSFLVFS YVPFSAFGPV
VEPSDAPDAF LTEDPRAVIK SGKFAQVPWA VTYTTEDGGY NAAQLLERNK LTGESWIDLL
NDRWFDWAPY LLFYRDAKKT IKDMDDLSFD LRQQYLADRR FSVESYWNVQ RMFTDVLFKN
SVPSAIDLHR KYGKSPVYSF VYDNPTDSGV GQLLSNRTDV HFGTVHGDDF FLIFNTAAYR
TGIRPDEEVI SKKFIGMLED FALNDKGTLT FGECNFQNNV NSKEYQVLRI SRNACKNEEY
ARFP
