ESXA_MYCBO
ID ESXA_MYCBO Reviewed; 95 AA.
AC P0A565; A0A1R3Y5H8; O84901; Q57165; X2BPJ5;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=6 kDa early secretory antigenic target;
DE AltName: Full=ESAT-6;
GN Name=esxA; Synonyms=esaT6; OrderedLocusNames=BQ2027_MB3905;
OS Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=233413;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8631702; DOI=10.1128/jb.178.5.1274-1282.1996;
RA Mahairas G.G., Sabo P.J., Hickey M.J., Singh D.C., Stover C.K.;
RT "Molecular analysis of genetic differences between Mycobacterium bovis BCG
RT and virulent M. bovis.";
RL J. Bacteriol. 178:1274-1282(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT "The complete genome sequence of Mycobacterium bovis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA Robbe-Austerman S., Gordon S.V.;
RT "Updated reference genome sequence and annotation of Mycobacterium bovis
RT AF2122/97.";
RL Genome Announc. 5:E00157-E00157(2017).
CC -!- FUNCTION: A secreted protein. Acts as a strong host T-cell antigen.
CC Plays a number of roles in modulating the host's immune response to
CC infection as well as being responsible for bacterial escape into the
CC host cytoplasm. {ECO:0000250|UniProtKB:P9WNK7}.
CC -!- SUBUNIT: Forms a tight 1:1 complex with EsxB (CFP-10).
CC {ECO:0000250|UniProtKB:P9WNK7}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P9WNK7}. Host
CC membrane {ECO:0000250|UniProtKB:P9WNK7}. Note=Probably secreted via the
CC ESX-1 / type VII secretion system (T7SS). A central pair of alpha
CC helices probably insert into host membrane.
CC {ECO:0000250|UniProtKB:P9WNK7}.
CC -!- MISCELLANEOUS: Genes esxA and esxB are part of RD1 (part of a 15-gene
CC locus known as ESX-1), a section of DNA deleted in the M.bovis BCG
CC strain used for vaccination. Deletion of this region is thought to be
CC largely responsible for the attenuation of BCG, and esxA and esxB in
CC particular are very important in this effect (PubMed:8631702).
CC {ECO:0000305|PubMed:8631702}.
CC -!- SIMILARITY: Belongs to the WXG100 family. ESAT-6 subfamily.
CC {ECO:0000305}.
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DR EMBL; U34848; AAC44033.1; -; Genomic_DNA.
DR EMBL; LT708304; SIU02537.1; -; Genomic_DNA.
DR RefSeq; NP_857542.1; NC_002945.3.
DR RefSeq; WP_003399963.1; NC_002945.4.
DR AlphaFoldDB; P0A565; -.
DR BMRB; P0A565; -.
DR SMR; P0A565; -.
DR EnsemblBacteria; SIU02537; SIU02537; BQ2027_MB3905.
DR GeneID; 45427879; -.
DR PATRIC; fig|233413.5.peg.4278; -.
DR OMA; QAVQMRW; -.
DR Proteomes; UP000001419; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR036689; ESAT-6-like_sf.
DR InterPro; IPR010310; T7SS_ESAT-6-like.
DR Pfam; PF06013; WXG100; 1.
DR SUPFAM; SSF140453; SSF140453; 1.
DR TIGRFAMs; TIGR03930; WXG100_ESAT6; 1.
PE 3: Inferred from homology;
KW Coiled coil; Host membrane; Membrane; Secreted; Transmembrane;
KW Transmembrane helix; Virulence.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..95
FT /note="6 kDa early secretory antigenic target"
FT /id="PRO_0000167791"
FT TRANSMEM 11..43
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TRANSMEM 49..85
FT /note="Helical"
FT /evidence="ECO:0000305"
FT COILED 56..87
FT /evidence="ECO:0000255"
SQ SEQUENCE 95 AA; 9904 MW; 79BD529E3D88F519 CRC64;
MTEQQWNFAG IEAAASAIQG NVTSIHSLLD EGKQSLTKLA AAWGGSGSEA YQGVQQKWDA
TATELNNALQ NLARTISEAG QAMASTEGNV TGMFA
