ID   ESXA_MYCTO              Reviewed;          95 AA.
AC   P9WNK6; F2GDN6; O84901; P0A564; Q540D8; Q57165;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=6 kDa early secretory antigenic target;
DE   AltName: Full=ESAT-6;
GN   Name=esxA; Synonyms=esaT6; OrderedLocusNames=MT3989;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: A secreted protein. Acts as a strong host T-cell antigen.
CC       Plays a number of roles in modulating the host's immune response to
CC       infection as well as being responsible for bacterial escape into the
CC       host cytoplasm. {ECO:0000250|UniProtKB:P9WNK7}.
CC   -!- SUBUNIT: Forms a tight 1:1 complex with EsxB (CFP-10).
CC       {ECO:0000250|UniProtKB:P9WNK7}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P9WNK7}. Host
CC       membrane {ECO:0000250|UniProtKB:P9WNK7}. Note=Probably secreted via the
CC       ESX-1 / type VII secretion system (T7SS). A central pair of alpha
CC       helices probably insert into host membrane.
CC       {ECO:0000250|UniProtKB:P9WNK7}.
CC   -!- SIMILARITY: Belongs to the WXG100 family. ESAT-6 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK48357.1; -; Genomic_DNA.
DR   PIR; A70803; A70803.
DR   RefSeq; WP_003399963.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WNK6; -.
DR   BMRB; P9WNK6; -.
DR   SMR; P9WNK6; -.
DR   EnsemblBacteria; AAK48357; AAK48357; MT3989.
DR   GeneID; 45427879; -.
DR   KEGG; mtc:MT3989; -.
DR   PATRIC; fig|83331.31.peg.4291; -.
DR   HOGENOM; CLU_151185_3_4_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR036689; ESAT-6-like_sf.
DR   InterPro; IPR010310; T7SS_ESAT-6-like.
DR   Pfam; PF06013; WXG100; 1.
DR   SUPFAM; SSF140453; SSF140453; 1.
DR   TIGRFAMs; TIGR03930; WXG100_ESAT6; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Host membrane; Membrane; Secreted; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..95
FT                   /note="6 kDa early secretory antigenic target"
FT                   /id="PRO_0000427110"
FT   TRANSMEM        11..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        49..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000305"
FT   COILED          56..87
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   95 AA;  9904 MW;  79BD529E3D88F519 CRC64;
     MTEQQWNFAG IEAAASAIQG NVTSIHSLLD EGKQSLTKLA AAWGGSGSEA YQGVQQKWDA
     TATELNNALQ NLARTISEAG QAMASTEGNV TGMFA