ID   ESXA_STAA3              Reviewed;          97 AA.
AC   A0A0H2XI99;
DT   05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Type VII secretion system extracellular protein A {ECO:0000305};
DE            Short=Ess extracellular protein A {ECO:0000305};
GN   Name=esxA {ECO:0000303|PubMed:24033479};
GN   OrderedLocusNames=SAUSA300_0278 {ECO:0000312|EMBL:ABD21786.1};
OS   Staphylococcus aureus (strain USA300).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=367830;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=USA300;
RX   PubMed=16517273; DOI=10.1016/s0140-6736(06)68231-7;
RA   Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G.,
RA   Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F.,
RA   Perdreau-Remington F.;
RT   "Complete genome sequence of USA300, an epidemic clone of community-
RT   acquired meticillin-resistant Staphylococcus aureus.";
RL   Lancet 367:731-739(2006).
RN   [2]
RP   SUBUNIT, AND SUBCELLULAR LOCATION.
RC   STRAIN=USA300;
RX   PubMed=24033479; DOI=10.1111/mmi.12395;
RA   Anderson M., Aly K.A., Chen Y.H., Missiakas D.;
RT   "Secretion of atypical protein substrates by the ESAT-6 secretion system of
RT   Staphylococcus aureus.";
RL   Mol. Microbiol. 90:734-743(2013).
RN   [3]
RP   FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF 90-LEU--GLN-97, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=USA300;
RX   PubMed=25047846; DOI=10.1128/iai.01576-14;
RA   Korea C.G., Balsamo G., Pezzicoli A., Merakou C., Tavarini S., Bagnoli F.,
RA   Serruto D., Unnikrishnan M.;
RT   "Staphylococcal Esx proteins modulate apoptosis and release of
RT   intracellular Staphylococcus aureus during infection in epithelial cells.";
RL   Infect. Immun. 82:4144-4153(2014).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=USA300;
RX   PubMed=28785545; DOI=10.3389/fcimb.2017.00330;
RA   Cruciani M., Etna M.P., Camilli R., Giacomini E., Percario Z.A., Severa M.,
RA   Sandini S., Rizzo F., Brandi V., Balsamo G., Polticelli F., Affabris E.,
RA   Pantosti A., Bagnoli F., Coccia E.M.;
RT   "Staphylococcus aureus Esx Factors Control Human Dendritic Cell Functions
RT   Conditioning Th1/Th17 Response.";
RL   Front. Cell. Infect. Microbiol. 7:330-330(2017).
CC   -!- FUNCTION: Virulence factor that is important for the establishment of
CC       infection in the host. EsxA is required for EsxB synthesis as well as
CC       secretion (By similarity). Modulates host cell apoptotic pathways and
CC       mediates together with EsxB the release of S.aureus from the host cell
CC       (PubMed:25047846). By acting on apoptosis, plays a role in the
CC       modulation of dendritic cell-mediated immunity (PubMed:28785545).
CC       {ECO:0000250|UniProtKB:P0C046, ECO:0000269|PubMed:25047846,
CC       ECO:0000269|PubMed:28785545}.
CC   -!- SUBUNIT: Forms both homodimers and heterodimers with EsxC.
CC       {ECO:0000269|PubMed:24033479}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24033479,
CC       ECO:0000269|PubMed:25047846}. Note=Secreted via the ESAT-6 secretion
CC       system (Ess) / type VII secretion system (T7SS). {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Significant increased of early and late apoptotic
CC       host cells including infected epithelial cells or dendritic cells.
CC       {ECO:0000269|PubMed:25047846, ECO:0000269|PubMed:28785545}.
CC   -!- SIMILARITY: Belongs to the WXG100 family. sagEsxA-like subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CP000255; ABD21786.1; -; Genomic_DNA.
DR   RefSeq; WP_001240826.1; NZ_CP027476.1.
DR   AlphaFoldDB; A0A0H2XI99; -.
DR   SMR; A0A0H2XI99; -.
DR   EnsemblBacteria; ABD21786; ABD21786; SAUSA300_0278.
DR   GeneID; 66838585; -.
DR   KEGG; saa:SAUSA300_0278; -.
DR   HOGENOM; CLU_158563_4_0_9; -.
DR   OMA; SDLKGMW; -.
DR   Proteomes; UP000001939; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR036689; ESAT-6-like_sf.
DR   InterPro; IPR010310; T7SS_ESAT-6-like.
DR   Pfam; PF06013; WXG100; 1.
DR   SUPFAM; SSF140453; SSF140453; 1.
DR   TIGRFAMs; TIGR03930; WXG100_ESAT6; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Secreted; Virulence.
FT   CHAIN           1..97
FT                   /note="Type VII secretion system extracellular protein A"
FT                   /id="PRO_0000437372"
FT   COILED          61..93
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         90..97
FT                   /note="Missing: Almost 75% loss of secretion."
FT                   /evidence="ECO:0000269|PubMed:25047846"
SQ   SEQUENCE   97 AA;  11036 MW;  E771B823D839B108 CRC64;
     MAMIKMSPEE IRAKSQSYGQ GSDQIRQILS DLTRAQGEIA ANWEGQAFSR FEEQFQQLSP
     KVEKFAQLLE EIKQQLNSTA DAVQEQDQQL SNNFGLQ