ID   ESXA_THECD              Reviewed;          97 AA.
AC   D1A4H1;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   19-JAN-2010, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=ESAT-6-like protein EsxA {ECO:0000303|PubMed:25865481};
GN   Name=esxA {ECO:0000303|PubMed:25865481}; OrderedLocusNames=Tcur_0611;
OS   Thermomonospora curvata (strain ATCC 19995 / DSM 43183 / JCM 3096 / KCTC
OS   9072 / NBRC 15933 / NCIMB 10081 / Henssen B9).
OC   Bacteria; Actinobacteria; Streptosporangiales; Thermomonosporaceae;
OC   Thermomonospora.
OX   NCBI_TaxID=471852;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19995 / DSM 43183 / JCM 3096 / KCTC 9072 / NBRC 15933 / NCIMB
RC   10081 / Henssen B9;
RX   PubMed=21475583; DOI=10.4056/sigs.1453580;
RA   Chertkov O., Sikorski J., Nolan M., Lapidus A., Lucas S., Del Rio T.G.,
RA   Tice H., Cheng J.F., Goodwin L., Pitluck S., Liolios K., Ivanova N.,
RA   Mavromatis K., Mikhailova N., Ovchinnikova G., Pati A., Chen A.,
RA   Palaniappan K., Djao O.D., Land M., Hauser L., Chang Y.J., Jeffries C.D.,
RA   Brettin T., Han C., Detter J.C., Rohde M., Goeker M., Woyke T., Bristow J.,
RA   Eisen J.A., Markowitz V., Hugenholtz P., Klenk H.P., Kyrpides N.C.;
RT   "Complete genome sequence of Thermomonospora curvata type strain (B9).";
RL   Stand. Genomic Sci. 1:13-22(2011).
RN   [2] {ECO:0007744|PDB:4LWS}
RP   X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 2-97 IN COMPLEX WITH ESXB, AND
RP   INTERACTION WITH ESXB.
RC   STRAIN=ATCC 19995 / DSM 43183 / JCM 3096 / KCTC 9072 / NBRC 15933 / NCIMB
RC   10081 / Henssen B9;
RX   PubMed=25865481; DOI=10.1016/j.cell.2015.03.040;
RA   Rosenberg O.S., Dovala D., Li X., Connolly L., Bendebury A.,
RA   Finer-Moore J., Holton J., Cheng Y., Stroud R.M., Cox J.S.;
RT   "Substrates control multimerization and activation of the multi-domain
RT   ATPase motor of type VII secretion.";
RL   Cell 161:501-512(2015).
CC   -!- FUNCTION: May help regulate assembly and function of the type VII
CC       secretion system (T7SS) (PubMed:25865481). EsxA disassembles pre-formed
CC       EccC-EsxB multimers, possibly by making EccC-EsxA-EsxB trimers instead
CC       of EccC-EsxB-EsxB-EccC tetramers (PubMed:25865481).
CC       {ECO:0000269|PubMed:25865481}.
CC   -!- SUBUNIT: Forms a tight 1:1 complex with EsxB (PubMed:25865481). Forms a
CC       complex with EccC and EsxB, probably wholly mediated by EsxB
CC       (PubMed:25865481). {ECO:0000269|PubMed:25865481}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P9WNK7}.
CC       Note=Probably secreted via the ESX / type VII secretion system (T7SS).
CC       {ECO:0000305|PubMed:25865481}.
CC   -!- SIMILARITY: Belongs to the WXG100 family. ESAT-6 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CP001738; ACY96206.1; -; Genomic_DNA.
DR   RefSeq; WP_012850990.1; NC_013510.1.
DR   PDB; 4LWS; X-ray; 2.00 A; B=2-97.
DR   PDBsum; 4LWS; -.
DR   AlphaFoldDB; D1A4H1; -.
DR   SMR; D1A4H1; -.
DR   STRING; 471852.Tcur_0611; -.
DR   EnsemblBacteria; ACY96206; ACY96206; Tcur_0611.
DR   KEGG; tcu:Tcur_0611; -.
DR   eggNOG; COG4842; Bacteria.
DR   HOGENOM; CLU_151185_3_2_11; -.
DR   OMA; PLQQTWT; -.
DR   OrthoDB; 1930308at2; -.
DR   Proteomes; UP000001918; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR036689; ESAT-6-like_sf.
DR   InterPro; IPR010310; T7SS_ESAT-6-like.
DR   Pfam; PF06013; WXG100; 1.
DR   SUPFAM; SSF140453; SSF140453; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome; Secreted.
FT   CHAIN           1..97
FT                   /note="ESAT-6-like protein EsxA"
FT                   /id="PRO_0000438301"
FT   HELIX           2..38
FT                   /evidence="ECO:0007829|PDB:4LWS"
FT   HELIX           39..43
FT                   /evidence="ECO:0007829|PDB:4LWS"
FT   HELIX           46..80
FT                   /evidence="ECO:0007829|PDB:4LWS"
SQ   SEQUENCE   97 AA;  10830 MW;  8E79F5E48C4C8A62 CRC64;
     MSDYTRANFG GLSEGEAQFS MTARALLDEL TDLEGKLRAK LDRWDGDAQA AYWNYQKEWD
     AAAKDMQNVV AQLGVAIREA HDNYQAAERA NTSIWAG