ID   ESXB_STAA3              Reviewed;         104 AA.
AC   A0A0H2XIE9;
DT   05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   25-MAY-2022, entry version 20.
DE   RecName: Full=Type VII secretion system extracellular protein B {ECO:0000305};
DE            Short=Ess extracellular protein B {ECO:0000305};
GN   Name=esxB {ECO:0000303|PubMed:24033479};
GN   OrderedLocusNames=SAUSA300_0285 {ECO:0000312|EMBL:ABD21833.1};
OS   Staphylococcus aureus (strain USA300).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=367830;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=USA300;
RX   PubMed=16517273; DOI=10.1016/s0140-6736(06)68231-7;
RA   Diep B.A., Gill S.R., Chang R.F., Phan T.H., Chen J.H., Davidson M.G.,
RA   Lin F., Lin J., Carleton H.A., Mongodin E.F., Sensabaugh G.F.,
RA   Perdreau-Remington F.;
RT   "Complete genome sequence of USA300, an epidemic clone of community-
RT   acquired meticillin-resistant Staphylococcus aureus.";
RL   Lancet 367:731-739(2006).
RN   [2]
RP   SUBUNIT, AND SUBCELLULAR LOCATION.
RC   STRAIN=USA300;
RX   PubMed=24033479; DOI=10.1111/mmi.12395;
RA   Anderson M., Aly K.A., Chen Y.H., Missiakas D.;
RT   "Secretion of atypical protein substrates by the ESAT-6 secretion system of
RT   Staphylococcus aureus.";
RL   Mol. Microbiol. 90:734-743(2013).
RN   [3]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC   STRAIN=USA300;
RX   PubMed=25047846; DOI=10.1128/iai.01576-14;
RA   Korea C.G., Balsamo G., Pezzicoli A., Merakou C., Tavarini S., Bagnoli F.,
RA   Serruto D., Unnikrishnan M.;
RT   "Staphylococcal Esx proteins modulate apoptosis and release of
RT   intracellular Staphylococcus aureus during infection in epithelial cells.";
RL   Infect. Immun. 82:4144-4153(2014).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=USA300;
RX   PubMed=28785545; DOI=10.3389/fcimb.2017.00330;
RA   Cruciani M., Etna M.P., Camilli R., Giacomini E., Percario Z.A., Severa M.,
RA   Sandini S., Rizzo F., Brandi V., Balsamo G., Polticelli F., Affabris E.,
RA   Pantosti A., Bagnoli F., Coccia E.M.;
RT   "Staphylococcus aureus Esx Factors Control Human Dendritic Cell Functions
RT   Conditioning Th1/Th17 Response.";
RL   Front. Cell. Infect. Microbiol. 7:330-330(2017).
RN   [5]
RP   INTERACTION WITH ESXD, AND MUTAGENESIS OF ASN-53.
RC   STRAIN=USA300;
RX   PubMed=29473132; DOI=10.1007/s12223-018-0591-6;
RA   Ibrahim A.M., Ragab Y.M., Aly K.A., Ramadan M.A.;
RT   "Error-prone PCR mutagenesis and reverse bacterial two-hybrid screening
RT   identify a mutation in asparagine 53 of the Staphylococcus aureus ESAT6-
RT   like component EsxB that perturbs interaction with EsxD.";
RL   Folia Microbiol. (Praha) 63:483-492(2018).
CC   -!- FUNCTION: Virulence factor that is important for the establishment of
CC       infection in the host. EsxB is required for EsxA synthesis as well as
CC       secretion (By similarity). Mediates together with EsxA the release of
CC       S.aureus from the host cell (PubMed:25047846). Inhibits also host
CC       cytokine production and thus modulates dendritic cell-mediated immunity
CC       (PubMed:28785545). {ECO:0000250|UniProtKB:P0C047,
CC       ECO:0000269|PubMed:25047846, ECO:0000269|PubMed:28785545}.
CC   -!- SUBUNIT: Homodimer. When mixed with EsxA does not form heterodimers (By
CC       similarity). Forms heterodimers with EsxD.
CC       {ECO:0000250|UniProtKB:Q2G182, ECO:0000269|PubMed:24033479,
CC       ECO:0000269|PubMed:29473132}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24033479,
CC       ECO:0000269|PubMed:25047846}. Note=Secreted via the ESAT-6 secretion
CC       system (Ess) / type VII secretion system (T7SS). {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Increased capacity to promote cytokine release by
CC       host dendritic cells (PubMed:28785545). Does not modulate the apoptotic
CC       pathways of host cells (PubMed:25047846). {ECO:0000269|PubMed:25047846,
CC       ECO:0000269|PubMed:28785545}.
CC   -!- SIMILARITY: Belongs to the WXG100 family. {ECO:0000305}.
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DR   EMBL; CP000255; ABD21833.1; -; Genomic_DNA.
DR   RefSeq; WP_000509668.1; NZ_CP027476.1.
DR   AlphaFoldDB; A0A0H2XIE9; -.
DR   SMR; A0A0H2XIE9; -.
DR   EnsemblBacteria; ABD21833; ABD21833; SAUSA300_0285.
DR   GeneID; 66838592; -.
DR   KEGG; saa:SAUSA300_0285; -.
DR   HOGENOM; CLU_2248426_0_0_9; -.
DR   Proteomes; UP000001939; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR036689; ESAT-6-like_sf.
DR   InterPro; IPR010310; T7SS_ESAT-6-like.
DR   Pfam; PF06013; WXG100; 1.
DR   SUPFAM; SSF140453; SSF140453; 1.
PE   1: Evidence at protein level;
KW   Secreted; Virulence.
FT   CHAIN           1..104
FT                   /note="Type VII secretion system extracellular protein B"
FT                   /id="PRO_0000437373"
FT   MUTAGEN         53
FT                   /note="N->A: Complete loss of interaction with EsxD."
FT                   /evidence="ECO:0000269|PubMed:29473132"
SQ   SEQUENCE   104 AA;  11510 MW;  836DC8438CCFD80B CRC64;
     MGGYKGIKAD GGKVDQAKQL AAKTAKDIEA CQKQTQQLAE YIEGSDWEGQ FANKVKDVLL
     IMAKFQEELV QPMADHQKAI DNLSQNLAKY DTLSIKQGLD RVNP