ID   ESXH_MYCS2              Reviewed;          95 AA.
AC   A0QQ44;
DT   09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=ESAT-6-like protein EsxH {ECO:0000305};
GN   Name=esxH {ECO:0000303|PubMed:24803520};
GN   OrderedLocusNames=MSMEG_0621 {ECO:0000312|EMBL:ABK72724.1},
GN   MSMEI_0605 {ECO:0000312|EMBL:AFP37086.1};
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=19846780; DOI=10.1073/pnas.0900589106;
RA   Siegrist M.S., Unnikrishnan M., McConnell M.J., Borowsky M., Cheng T.Y.,
RA   Siddiqi N., Fortune S.M., Moody D.B., Rubin E.J.;
RT   "Mycobacterial Esx-3 is required for mycobactin-mediated iron
RT   acquisition.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:18792-18797(2009).
RN   [5]
RP   SUBCELLULAR LOCATION.
RX   PubMed=24803520; DOI=10.1128/mbio.01073-14;
RA   Siegrist M.S., Steigedal M., Ahmad R., Mehra A., Dragset M.S.,
RA   Schuster B.M., Philips J.A., Carr S.A., Rubin E.J.;
RT   "Mycobacterial Esx-3 requires multiple components for iron acquisition.";
RL   MBio 5:E01073-E01073(2014).
RN   [6] {ECO:0007744|PDB:3Q4H}
RP   X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) IN COMPLEX WITH ESXG.
RX   PubMed=24312350; DOI=10.1371/journal.pone.0081753;
RA   Arbing M.A., Chan S., Harris L., Kuo E., Zhou T.T., Ahn C.J., Nguyen L.,
RA   He Q., Lu J., Menchavez P.T., Shin A., Holton T., Sawaya M.R., Cascio D.,
RA   Eisenberg D.;
RT   "Heterologous expression of mycobacterial Esx complexes in Escherichia coli
RT   for structural studies is facilitated by the use of maltose binding protein
RT   fusions.";
RL   PLoS ONE 8:E81753-E81753(2013).
CC   -!- SUBUNIT: Forms a tight 1:1 complex with EsxG.
CC       {ECO:0000269|PubMed:24312350}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19846780,
CC       ECO:0000269|PubMed:24803520}. Note=Secreted via the ESX-3 / type VII
CC       secretion system (T7SS). {ECO:0000269|PubMed:19846780,
CC       ECO:0000269|PubMed:24803520}.
CC   -!- SIMILARITY: Belongs to the WXG100 family. ESAT-6 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CP000480; ABK72724.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP37086.1; -; Genomic_DNA.
DR   RefSeq; WP_003892041.1; NZ_SIJM01000009.1.
DR   RefSeq; YP_885032.1; NC_008596.1.
DR   PDB; 3Q4H; X-ray; 2.70 A; B/D=1-95.
DR   PDBsum; 3Q4H; -.
DR   AlphaFoldDB; A0QQ44; -.
DR   SMR; A0QQ44; -.
DR   STRING; 246196.MSMEI_0605; -.
DR   EnsemblBacteria; ABK72724; ABK72724; MSMEG_0621.
DR   EnsemblBacteria; AFP37086; AFP37086; MSMEI_0605.
DR   GeneID; 66738799; -.
DR   KEGG; msg:MSMEI_0605; -.
DR   KEGG; msm:MSMEG_0621; -.
DR   PATRIC; fig|246196.19.peg.617; -.
DR   eggNOG; COG4842; Bacteria.
DR   OMA; HEANTMS; -.
DR   OrthoDB; 1930308at2; -.
DR   EvolutionaryTrace; A0QQ44; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR036689; ESAT-6-like_sf.
DR   InterPro; IPR010310; T7SS_ESAT-6-like.
DR   Pfam; PF06013; WXG100; 1.
DR   SUPFAM; SSF140453; SSF140453; 1.
DR   TIGRFAMs; TIGR03930; WXG100_ESAT6; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome; Secreted.
FT   CHAIN           1..95
FT                   /note="ESAT-6-like protein EsxH"
FT                   /id="PRO_0000434999"
FT   HELIX           3..6
FT                   /evidence="ECO:0007829|PDB:3Q4H"
FT   HELIX           8..38
FT                   /evidence="ECO:0007829|PDB:3Q4H"
FT   HELIX           40..42
FT                   /evidence="ECO:0007829|PDB:3Q4H"
FT   STRAND          46..48
FT                   /evidence="ECO:0007829|PDB:3Q4H"
FT   HELIX           51..85
FT                   /evidence="ECO:0007829|PDB:3Q4H"
SQ   SEQUENCE   95 AA;  10470 MW;  54536AC935288017 CRC64;
     MSQIMYNYPA MLAHAAEMNT YSGALHAVGA DIAAEQHALA SAWQGDTGMT YQAWQAQWNQ
     AMEELVRAYR AMATTHEQNT MAMSARDQAE GAKWG