ID   ESXK_MYCTU              Reviewed;          98 AA.
AC   P9WNJ7; L0T8X9; O05299;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=ESAT-6-like protein EsxK {ECO:0000305};
GN   Name=esxK {ECO:0000303|PubMed:19876390}; OrderedLocusNames=Rv1197;
GN   ORFNames=MTCI364.09;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RX   PubMed=12919853; DOI=10.1016/s1286-4579(03)00179-5;
RA   He X.Y., Zhuang Y.H., Zhang X.G., Li G.L.;
RT   "Comparative proteome analysis of culture supernatant proteins of
RT   Mycobacterium tuberculosis H37Rv and H37Ra.";
RL   Microbes Infect. 5:851-856(2003).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=19876390; DOI=10.1371/journal.ppat.1000507;
RA   Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S.,
RA   Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C.,
RA   Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.;
RT   "Systematic genetic nomenclature for type VII secretion systems.";
RL   PLoS Pathog. 5:E1000507-E1000507(2009).
RN   [4]
RP   INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=22291682; DOI=10.3389/fmicb.2011.00266;
RA   Bukka A., Price C.T., Kernodle D.S., Graham J.E.;
RT   "Mycobacterium tuberculosis RNA expression patterns in sputum bacteria
RT   indicate secreted Esx factors contributing to growth are highly expressed
RT   in active disease.";
RL   Front. Microbiol. 2:266-266(2011).
RN   [5]
RP   SUBUNIT, AND INTERACTION WITH ESXL.
RC   STRAIN=H37Rv;
RX   PubMed=27751956; DOI=10.1016/j.bbagen.2016.10.013;
RA   Pandey H., Tripathi S., Srivastava K., Tripathi D.K., Srivastava M.,
RA   Kant S., Srivastava K.K., Arora A.;
RT   "Characterization of culture filtrate proteins Rv1197 and Rv1198 of ESAT-6
RT   family from Mycobacterium tuberculosis H37Rv.";
RL   Biochim. Biophys. Acta 1861:396-408(2017).
CC   -!- SUBUNIT: Strongly interacts with EsxL to form a heterodimeric complex
CC       under reducing conditions. The complex is regulated by the redox state
CC       of EsxL. {ECO:0000269|PubMed:27751956}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:12919853}.
CC       Note=Probably secreted via the ESX-5 / type VII secretion system
CC       (T7SS). {ECO:0000305|PubMed:19876390}.
CC   -!- INDUCTION: Differentially expressed under different growth conditions.
CC       Highly expressed in sputum bacteria. {ECO:0000269|PubMed:22291682}.
CC   -!- DISRUPTION PHENOTYPE: EsxKL deletion mutant shows reduced intracellular
CC       growth in primary human macrophages. Also shows growth defects in the
CC       absence of host cells. {ECO:0000269|PubMed:22291682}.
CC   -!- SIMILARITY: Belongs to the WXG100 family. CFP-10 subfamily.
CC       {ECO:0000305|PubMed:19876390}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL123456; CCP43953.1; -; Genomic_DNA.
DR   PIR; C70608; C70608.
DR   RefSeq; NP_215713.1; NC_000962.3.
DR   RefSeq; WP_003421604.1; NZ_NVQJ01000025.1.
DR   AlphaFoldDB; P9WNJ7; -.
DR   SMR; P9WNJ7; -.
DR   STRING; 83332.Rv1197; -.
DR   PaxDb; P9WNJ7; -.
DR   DNASU; 886051; -.
DR   GeneID; 886051; -.
DR   KEGG; mtu:Rv1197; -.
DR   TubercuList; Rv1197; -.
DR   eggNOG; COG4842; Bacteria.
DR   PhylomeDB; P9WNJ7; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; HDA:MTBBASE.
DR   InterPro; IPR036689; ESAT-6-like_sf.
DR   InterPro; IPR010310; T7SS_ESAT-6-like.
DR   Pfam; PF06013; WXG100; 1.
DR   SUPFAM; SSF140453; SSF140453; 1.
DR   TIGRFAMs; TIGR03930; WXG100_ESAT6; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Secreted.
FT   CHAIN           1..98
FT                   /note="ESAT-6-like protein EsxK"
FT                   /id="PRO_0000167803"
SQ   SEQUENCE   98 AA;  10963 MW;  1FD04CAB64A779D1 CRC64;
     MASRFMTDPH AMRDMAGRFE VHAQTVEDEA RRMWASAQNI SGAGWSGMAE ATSLDTMAQM
     NQAFRNIVNM LHGVRDGLVR DANNYEQQEQ ASQQILSS