ID   ESXL_MYCTU              Reviewed;          94 AA.
AC   P9WNJ5; L0T8P7; O05300;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=ESAT-6-like protein EsxL {ECO:0000305};
GN   Name=esxL {ECO:0000303|PubMed:19876390}; OrderedLocusNames=Rv1198;
GN   ORFNames=MTCI364.10;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RX   PubMed=17443846; DOI=10.1002/pmic.200600853;
RA   Malen H., Berven F.S., Fladmark K.E., Wiker H.G.;
RT   "Comprehensive analysis of exported proteins from Mycobacterium
RT   tuberculosis H37Rv.";
RL   Proteomics 7:1702-1718(2007).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=19876390; DOI=10.1371/journal.ppat.1000507;
RA   Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S.,
RA   Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C.,
RA   Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.;
RT   "Systematic genetic nomenclature for type VII secretion systems.";
RL   PLoS Pathog. 5:E1000507-E1000507(2009).
RN   [4]
RP   INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=22291682; DOI=10.3389/fmicb.2011.00266;
RA   Bukka A., Price C.T., Kernodle D.S., Graham J.E.;
RT   "Mycobacterium tuberculosis RNA expression patterns in sputum bacteria
RT   indicate secreted Esx factors contributing to growth are highly expressed
RT   in active disease.";
RL   Front. Microbiol. 2:266-266(2011).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [6]
RP   FUNCTION IN VIRULENCE.
RC   STRAIN=H37Rv;
RX   PubMed=25242740; DOI=10.1016/j.bbrc.2014.09.047;
RA   Shi J., Zhang H., Fang L., Xi Y., Zhou Y., Luo R., Wang D., Xiao S.,
RA   Chen H.;
RT   "A novel firefly luciferase biosensor enhances the detection of apoptosis
RT   induced by ESAT-6 family proteins of Mycobacterium tuberculosis.";
RL   Biochem. Biophys. Res. Commun. 452:1046-1053(2014).
RN   [7]
RP   FUNCTION, IMMUNOGENICITY, SUBUNIT, INTERACTION WITH ESXK, AND SUBCELLULAR
RP   LOCATION.
RC   STRAIN=H37Rv;
RX   PubMed=27751956; DOI=10.1016/j.bbagen.2016.10.013;
RA   Pandey H., Tripathi S., Srivastava K., Tripathi D.K., Srivastava M.,
RA   Kant S., Srivastava K.K., Arora A.;
RT   "Characterization of culture filtrate proteins Rv1197 and Rv1198 of ESAT-6
RT   family from Mycobacterium tuberculosis H37Rv.";
RL   Biochim. Biophys. Acta 1861:396-408(2017).
CC   -!- FUNCTION: Induces apoptosis of host cells (PubMed:25242740). Is
CC       immunogenic with highly specific seroreactivity towards TB patients'
CC       serum (PubMed:27751956). {ECO:0000269|PubMed:25242740,
CC       ECO:0000269|PubMed:27751956}.
CC   -!- SUBUNIT: Strongly interacts with EsxK to form a heterodimeric complex
CC       under reducing conditions. The complex is regulated by the redox state
CC       of EsxL. {ECO:0000269|PubMed:27751956}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17443846}.
CC       Note=Probably secreted via the ESX-5 / type VII secretion system (T7SS)
CC       (Probable). Secretion may depend on complex formation with EsxK
CC       (Probable). {ECO:0000305|PubMed:19876390, ECO:0000305|PubMed:27751956}.
CC   -!- INDUCTION: Differentially expressed under different growth conditions.
CC       Highly expressed in sputum bacteria. {ECO:0000269|PubMed:22291682}.
CC   -!- DISRUPTION PHENOTYPE: EsxKL deletion mutant shows reduced intracellular
CC       growth in primary human macrophages. Also shows growth defects in the
CC       absence of host cells. {ECO:0000269|PubMed:22291682}.
CC   -!- MISCELLANEOUS: In BALB/c mice, immunization with EsxL-FIA induces a
CC       pro-inflammatory response with elevated levels of TNF and IL-6, along
CC       with low induction of IFN-gamma, IL-2 and IL-10, but no induction of
CC       IL-4. {ECO:0000269|PubMed:27751956}.
CC   -!- SIMILARITY: Belongs to the WXG100 family. ESAT-6 subfamily.
CC       {ECO:0000305|PubMed:19876390}.
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DR   EMBL; AL123456; CCP43954.1; -; Genomic_DNA.
DR   PIR; D70608; D70608.
DR   RefSeq; NP_215714.1; NC_000962.3.
DR   RefSeq; WP_003898766.1; NZ_NVQJ01000025.1.
DR   AlphaFoldDB; P9WNJ5; -.
DR   SMR; P9WNJ5; -.
DR   STRING; 83332.Rv1198; -.
DR   PaxDb; P9WNJ5; -.
DR   DNASU; 886090; -.
DR   GeneID; 886090; -.
DR   KEGG; mtu:Rv1198; -.
DR   TubercuList; Rv1198; -.
DR   eggNOG; ENOG5032I3T; Bacteria.
DR   PhylomeDB; P9WNJ5; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; HDA:MTBBASE.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   InterPro; IPR009416; ESAT-6-like_Myco.
DR   InterPro; IPR036689; ESAT-6-like_sf.
DR   InterPro; IPR010310; T7SS_ESAT-6-like.
DR   Pfam; PF06013; WXG100; 1.
DR   PIRSF; PIRSF037656; DUF1066; 1.
DR   SUPFAM; SSF140453; SSF140453; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Secreted; Virulence.
FT   CHAIN           1..94
FT                   /note="ESAT-6-like protein EsxL"
FT                   /id="PRO_0000167805"
SQ   SEQUENCE   94 AA;  9928 MW;  5585B30B9488C46C CRC64;
     MTINYQFGDV DAHGAMIRAQ AGLLEAEHQA IIRDVLTASD FWGGAGSAAC QGFITQLGRN
     FQVIYEQANA HGQKVQAAGN NMAQTDSAVG SSWA