ID   ESXR_MYCTU              Reviewed;          96 AA.
AC   P9WNI9; L0TBJ7; O53266; P64093;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=ESAT-6-like protein EsxR {ECO:0000305};
DE   AltName: Full=TB10.3 {ECO:0000312|EMBL:CCP45826.1};
GN   Name=esxR {ECO:0000303|PubMed:15336430, ECO:0000303|PubMed:19876390};
GN   OrderedLocusNames=Rv3019c; ORFNames=MTV012.33c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   INTERACTION WITH ESXS.
RX   PubMed=15336430; DOI=10.1111/j.1574-6968.2004.tb09764.x;
RA   Lightbody K.L., Renshaw P.S., Collins M.L., Wright R.L., Hunt D.M.,
RA   Gordon S.V., Hewinson R.G., Buxton R.S., Williamson R.A., Carr M.D.;
RT   "Characterisation of complex formation between members of the Mycobacterium
RT   tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding
RT   of the rules governing complex formation and thereby functional
RT   flexibility.";
RL   FEMS Microbiol. Lett. 238:255-262(2004).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=19876390; DOI=10.1371/journal.ppat.1000507;
RA   Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S.,
RA   Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C.,
RA   Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.;
RT   "Systematic genetic nomenclature for type VII secretion systems.";
RL   PLoS Pathog. 5:E1000507-E1000507(2009).
RN   [4] {ECO:0007744|PDB:3H6P}
RP   X-RAY CRYSTALLOGRAPHY (1.91 ANGSTROMS), SUBUNIT, AND DOMAIN.
RX   PubMed=20629176; DOI=10.1002/pro.451;
RA   Arbing M.A., Kaufmann M., Phan T., Chan S., Cascio D., Eisenberg D.;
RT   "The crystal structure of the Mycobacterium tuberculosis Rv3019c-Rv3020c
RT   ESX complex reveals a domain-swapped heterotetramer.";
RL   Protein Sci. 19:1692-1703(2010).
CC   -!- SUBUNIT: Forms a tight complex with EsxS (PubMed:15336430). Exists in
CC       heterodimeric and heterotetrameric forms (PubMed:20629176).
CC       {ECO:0000269|PubMed:15336430, ECO:0000269|PubMed:20629176}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:19876390}.
CC       Note=Probably secreted via the ESX-3 / type VII secretion system
CC       (T7SS). {ECO:0000305|PubMed:19876390}.
CC   -!- DOMAIN: In the heterotetrameric form, EsxS adopts a long helical
CC       conformation that pairs with a second EsxS monomer in an antiparallel
CC       manner that allows the N- and C-termini of two EsxS subunits to
CC       interact with an EsxR subunit at each end of the EsxS dimer thus
CC       creating a molecule with four-helix bundles at its extremities.
CC       {ECO:0000269|PubMed:20629176}.
CC   -!- SIMILARITY: Belongs to the WXG100 family. ESAT-6 subfamily.
CC       {ECO:0000305|PubMed:19876390}.
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DR   EMBL; AL123456; CCP45826.1; -; Genomic_DNA.
DR   PIR; F70857; F70857.
DR   RefSeq; NP_217535.1; NC_000962.3.
DR   RefSeq; WP_003415340.1; NZ_NVQJ01000114.1.
DR   PDB; 3H6P; X-ray; 1.91 A; C/D=1-96.
DR   PDBsum; 3H6P; -.
DR   AlphaFoldDB; P9WNI9; -.
DR   SMR; P9WNI9; -.
DR   STRING; 83332.Rv3019c; -.
DR   PaxDb; P9WNI9; -.
DR   DNASU; 888926; -.
DR   GeneID; 888926; -.
DR   KEGG; mtu:Rv3019c; -.
DR   TubercuList; Rv3019c; -.
DR   eggNOG; COG4842; Bacteria.
DR   OMA; AVWHGDT; -.
DR   PhylomeDB; P9WNI9; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0032991; C:protein-containing complex; IDA:MTBBASE.
DR   InterPro; IPR036689; ESAT-6-like_sf.
DR   InterPro; IPR010310; T7SS_ESAT-6-like.
DR   Pfam; PF06013; WXG100; 1.
DR   SUPFAM; SSF140453; SSF140453; 1.
DR   TIGRFAMs; TIGR03930; WXG100_ESAT6; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome; Secreted.
FT   CHAIN           1..96
FT                   /note="ESAT-6-like protein EsxR"
FT                   /id="PRO_0000167813"
FT   HELIX           21..38
FT                   /evidence="ECO:0007829|PDB:3H6P"
FT   HELIX           40..42
FT                   /evidence="ECO:0007829|PDB:3H6P"
FT   STRAND          47..49
FT                   /evidence="ECO:0007829|PDB:3H6P"
FT   HELIX           51..73
FT                   /evidence="ECO:0007829|PDB:3H6P"
SQ   SEQUENCE   96 AA;  10312 MW;  6BA858B058854D46 CRC64;
     MSQIMYNYPA MMAHAGDMAG YAGTLQSLGA DIASEQAVLS SAWQGDTGIT YQGWQTQWNQ
     ALEDLVRAYQ SMSGTHESNT MAMLARDGAE AAKWGG