ID   ESXS_MYCTU              Reviewed;          97 AA.
AC   Q6MX18; F2GP49; I6Y2A8;
DT   07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=ESAT-6-like protein EsxS {ECO:0000305};
GN   Name=esxS {ECO:0000303|PubMed:19876390};
GN   OrderedLocusNames=Rv3020c {ECO:0000312|EMBL:CCP45827.1};
GN   ORFNames=LH57_16495 {ECO:0000312|EMBL:AIR15800.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA   Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA   Monaco A., King S., Sohrabi A.;
RT   "Phylogenetic analysis of Mycobacterial species using whole genome
RT   sequences.";
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   INTERACTION WITH ESXR.
RX   PubMed=15336430; DOI=10.1111/j.1574-6968.2004.tb09764.x;
RA   Lightbody K.L., Renshaw P.S., Collins M.L., Wright R.L., Hunt D.M.,
RA   Gordon S.V., Hewinson R.G., Buxton R.S., Williamson R.A., Carr M.D.;
RT   "Characterisation of complex formation between members of the Mycobacterium
RT   tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding
RT   of the rules governing complex formation and thereby functional
RT   flexibility.";
RL   FEMS Microbiol. Lett. 238:255-262(2004).
RN   [4]
RP   NOMENCLATURE.
RX   PubMed=19876390; DOI=10.1371/journal.ppat.1000507;
RA   Bitter W., Houben E.N., Bottai D., Brodin P., Brown E.J., Cox J.S.,
RA   Derbyshire K., Fortune S.M., Gao L.Y., Liu J., Gey van Pittius N.C.,
RA   Pym A.S., Rubin E.J., Sherman D.R., Cole S.T., Brosch R.;
RT   "Systematic genetic nomenclature for type VII secretion systems.";
RL   PLoS Pathog. 5:E1000507-E1000507(2009).
RN   [5] {ECO:0007744|PDB:3H6P}
RP   X-RAY CRYSTALLOGRAPHY (1.91 ANGSTROMS), SUBUNIT, AND DOMAIN.
RX   PubMed=20629176; DOI=10.1002/pro.451;
RA   Arbing M.A., Kaufmann M., Phan T., Chan S., Cascio D., Eisenberg D.;
RT   "The crystal structure of the Mycobacterium tuberculosis Rv3019c-Rv3020c
RT   ESX complex reveals a domain-swapped heterotetramer.";
RL   Protein Sci. 19:1692-1703(2010).
CC   -!- SUBUNIT: Forms a tight complex with EsxR (PubMed:15336430). Exists in
CC       heterodimeric and heterotetrameric forms (PubMed:20629176).
CC       {ECO:0000269|PubMed:15336430, ECO:0000269|PubMed:20629176}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:19876390}.
CC       Note=Probably secreted via the ESX-3 / type VII secretion system
CC       (T7SS). {ECO:0000305|PubMed:19876390}.
CC   -!- DOMAIN: In the heterotetrameric form, EsxS adopts a long helical
CC       conformation that pairs with a second EsxS monomer in an antiparallel
CC       manner that allows the N- and C-termini of two EsxS subunits to
CC       interact with an EsxR subunit at each end of the EsxS dimer thus
CC       creating a molecule with four-helix bundles at its extremities.
CC       {ECO:0000269|PubMed:20629176}.
CC   -!- SIMILARITY: Belongs to the WXG100 family. CFP-10 subfamily.
CC       {ECO:0000305|PubMed:19876390}.
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DR   EMBL; CP009480; AIR15800.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP45827.1; -; Genomic_DNA.
DR   RefSeq; WP_003415342.1; NZ_NVQJ01000114.1.
DR   RefSeq; YP_177919.1; NC_000962.3.
DR   PDB; 3H6P; X-ray; 1.91 A; A/B=1-97.
DR   PDBsum; 3H6P; -.
DR   AlphaFoldDB; Q6MX18; -.
DR   SMR; Q6MX18; -.
DR   STRING; 83332.Rv3020c; -.
DR   PaxDb; Q6MX18; -.
DR   DNASU; 888946; -.
DR   GeneID; 45427013; -.
DR   GeneID; 888946; -.
DR   KEGG; mtu:Rv3020c; -.
DR   PATRIC; fig|83332.111.peg.3366; -.
DR   TubercuList; Rv3020c; -.
DR   eggNOG; ENOG5030NJS; Bacteria.
DR   HOGENOM; CLU_161983_0_0_11; -.
DR   OMA; ASHARFI; -.
DR   PhylomeDB; Q6MX18; -.
DR   EvolutionaryTrace; Q6MX18; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0032991; C:protein-containing complex; IDA:MTBBASE.
DR   InterPro; IPR036689; ESAT-6-like_sf.
DR   InterPro; IPR010310; T7SS_ESAT-6-like.
DR   Pfam; PF06013; WXG100; 1.
DR   SUPFAM; SSF140453; SSF140453; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome; Secreted.
FT   CHAIN           1..97
FT                   /note="ESAT-6-like protein EsxS"
FT                   /id="PRO_0000436932"
FT   HELIX           18..74
FT                   /evidence="ECO:0007829|PDB:3H6P"
FT   HELIX           77..79
FT                   /evidence="ECO:0007829|PDB:3H6P"
SQ   SEQUENCE   97 AA;  9842 MW;  BA9BCB3180EC17F2 CRC64;
     MSLLDAHIPQ LIASHTAFAA KAGLMRHTIG QAEQQAMSAQ AFHQGESAAA FQGAHARFVA
     AAAKVNTLLD IAQANLGEAA GTYVAADAAA ASSYTGF